2025
Fast Actin Disassembly and Fimbrin Mechanosensitivity Support Rapid Turnover in a Model of Clathrin‐Mediated Endocytosis
Mousavi S, Lacy M, Li X, Berro J. Fast Actin Disassembly and Fimbrin Mechanosensitivity Support Rapid Turnover in a Model of Clathrin‐Mediated Endocytosis. Cytoskeleton 2025 PMID: 40035221, DOI: 10.1002/cm.22002.Peer-Reviewed Original ResearchClathrin-mediated endocytosisActin filament disassemblyDynamics of actinActin-interacting proteinHigh membrane tensionActin meshworkEndocytic proteinsFilament disassemblyActin disassemblyNascent filamentsActin cytoskeletonEndocytic structuresEukaryotic cellsBinding partnersCellular processesTurgor pressureFimbrinActinRapid turnoverMembrane tensionEndocytosisProteinFilamentsDisassemblyYeastSingle-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF
Cheppali S, Li C, Xing W, Sun R, Yang M, Xue Y, Lu S, Yao J, Sun S, Chen C, Sui S. Single-molecule two- and three-colour FRET studies reveal a transition state in SNARE disassembly by NSF. Nature Communications 2025, 16: 250. PMID: 39747074, PMCID: PMC11695992, DOI: 10.1038/s41467-024-55531-0.Peer-Reviewed Original ResearchConceptsN-ethylmaleimide sensitive factorSNARE complexDisassembly of SNARE complexesStable four-helix bundleFour-helix bundleSNARE motifFluorescence spectroscopy approachesMinimal machineryAAA+ ATPasesProtein machineryAdapter proteinVesicle fusionMembrane fusionSyntaxinPhysiological processesSnareProteinN-ethylmaleimideDisassemblySequential disassemblyMachinerySequential pathwayPathwayEukaryotesFusion
2024
Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers
Li F, Coleman J, Redondo-Morata L, Kalyana Sundaram R, Stroeva E, Rothman J, Pincet F. Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers. Communications Biology 2024, 7: 1608. PMID: 39627539, PMCID: PMC11615320, DOI: 10.1038/s42003-024-07317-9.Peer-Reviewed Original ResearchConceptsSyt-1Lipid bilayerRing-like oligomersCa2+-evoked releaseSynaptotagmin-1Single-molecule imaging methodsSynaptic vesiclesBiochemical evidencePhysiological requirementsOligomerizationAnalysis of assembliesBilayerOligomersCa2+LipidAssemblyCa2Classes of oligomersMutationsVesiclesDisassemblyEvoked release
2022
Retro-2 alters Golgi structure
Yue X, Gim B, Zhu L, Tan C, Qian Y, Graham M, Liu X, Lee I. Retro-2 alters Golgi structure. Scientific Reports 2022, 12: 14975. PMID: 36056100, PMCID: PMC9438350, DOI: 10.1038/s41598-022-19415-x.Peer-Reviewed Original ResearchConceptsRetro-2Golgi apparatusGolgi structureER exit site proteinGolgi structural proteinsGolgi stacksER exitGolgi organizationIndividual cisternaeGolgiStructural proteinsProtein secretionPerinuclear areaUltra-structural studiesEM tomographyVesicular profilesHuman lung cellsPartial disassemblySyntaxin5ProteinCultured human lung cellsCell depletionDisassemblyUltrastructureCells
2012
Structural characterization of full-length NSF and 20S particles
Chang L, Chen S, Liu C, Pan X, Jiang J, Bai X, Xie X, Wang H, Sui S. Structural characterization of full-length NSF and 20S particles. Nature Structural & Molecular Biology 2012, 19: 268-275. PMID: 22307055, DOI: 10.1038/nsmb.2237.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Vesicular TransportAdenosine DiphosphateAnimalsCricetinaeCricetulusCryoelectron MicroscopyModels, MolecularN-Ethylmaleimide-Sensitive ProteinsProtein BindingProtein Structure, QuaternaryProtein Structure, TertiarySNARE ProteinsStructural Homology, ProteinSubstrate Specificity
2011
Dynamic molecular processes mediate cellular mechanotransduction
Hoffman BD, Grashoff C, Schwartz MA. Dynamic molecular processes mediate cellular mechanotransduction. Nature 2011, 475: 316-323. PMID: 21776077, PMCID: PMC6449687, DOI: 10.1038/nature10316.Peer-Reviewed Original ResearchConceptsCell adhesion complexesDistinct signaling pathwaysTransduction of forceDynamic molecular processesEmbryonic developmentCellular mechanotransductionPlasma membraneBiochemical signalsAdult physiologySignaling pathwaysCellular responsesSubcellular structuresMolecular processesNumerous diseasesMechanical forcesMuscular dystrophyCytoskeletonTransductionMechanotransductionPathwayPhysiologyDisassemblyRecent workMembraneAssembly
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