2025
Biomimetic membrane in a microfluidic chip for the electrical and optical monitoring of biological reactions
Mion D, Bunel L, Ramakrishnan S, Heo P, Pincet F. Biomimetic membrane in a microfluidic chip for the electrical and optical monitoring of biological reactions. Nature Protocols 2025, 1-32. PMID: 40461799, DOI: 10.1038/s41596-025-01171-7.Peer-Reviewed Original ResearchMicrofluidic chipTwo-dimensional bilayersBiomimetic membranesChip fabricationSingle-particleMicrofluidic channelOptical microscopyElectrical readoutResin moldMicrofluidic setupMembrane typeOptical monitoringChipPoly(dimethylsiloxaneBiological membranesPhysicochemical cuesFluid lipidsBilayerCharacteristics of biological membranesChannelComprehensive procedureBilayer leafletsMolecular reactions
2024
Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers
Li F, Coleman J, Redondo-Morata L, Kalyana Sundaram R, Stroeva E, Rothman J, Pincet F. Quantitative single-molecule analysis of assembly and Ca2+-dependent disassembly of synaptotagmin oligomers on lipid bilayers. Communications Biology 2024, 7: 1608. PMID: 39627539, PMCID: PMC11615320, DOI: 10.1038/s42003-024-07317-9.Peer-Reviewed Original ResearchConceptsSyt-1Lipid bilayerRing-like oligomersCa2+-evoked releaseSynaptotagmin-1Single-molecule imaging methodsSynaptic vesiclesBiochemical evidencePhysiological requirementsOligomerizationAnalysis of assembliesBilayerOligomersCa2+LipidAssemblyCa2Classes of oligomersMutationsVesiclesDisassemblyEvoked release
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