2015
Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and piggyBac transposase lineage labeling
Chen F, Rosiene J, Che A, Becker A, LoTurco J. Tracking and transforming neocortical progenitors by CRISPR/Cas9 gene targeting and piggyBac transposase lineage labeling. Development 2015, 142: 3601-3611. PMID: 26400094, PMCID: PMC4631763, DOI: 10.1242/dev.118836.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAstrocytesBrainBrain NeoplasmsCell LineageChromosomes, Artificial, BacterialCRISPR-Cas SystemsElectroporationFemaleGlioblastomaHumansMutagenesisMutationNeurofibromin 1NeuronsPregnancyPregnancy, AnimalPTEN PhosphohydrolaseRatsRats, WistarStem CellsTransposasesTumor Suppressor Protein p53ConceptsNeurodevelopmental disruptionNeural progenitorsLineage labelingCRISPR/Cas9 gene targetingNeuronal excitabilityDecreased PTEN expressionNeuronal hypertrophyNeocortical progenitorsModel human diseasesTumor formationSomatic mutationsTumorGlioblastoma tumorsPTEN expressionSomatic cellsTumor suppressorNeural developmentProgenitorsGene targetingInduce targeted mutationsHuman diseasesMutationsTargeted MutagenesisCRISPR/Cas9 systemCells
2008
Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis
Croda J, Figueira CP, Wunder EA, Santos CS, Reis MG, Ko AI, Picardeau M. Targeted Mutagenesis in Pathogenic Leptospira Species: Disruption of the LigB Gene Does Not Affect Virulence in Animal Models of Leptospirosis. Infection And Immunity 2008, 76: 5826-5833. PMID: 18809657, PMCID: PMC2583567, DOI: 10.1128/iai.00989-08.Peer-Reviewed Original ResearchConceptsPathogenic Leptospira speciesLeptospira speciesAcute disease manifestationsPersistent renal colonizationInoculation of ratsDisease manifestationsHamster modelRenal colonizationPathogenic mechanismsAnimal modelsPersistent colonizationDecrease virulenceLeptospira interrogansHomologous recombinationLeptospirosisVirulence factorsBacterial adherenceImmunoblot analysisCorresponding chromosomal locusWild-type strainCultured cellsFirst evidenceSpectinomycin resistance geneRepeat proteinsTargeted Mutagenesis
1997
Growth-Promoting Interaction of IGF-II with the Insulin Receptor during Mouse Embryonic Development
Louvi A, Accili D, Efstratiadis A. Growth-Promoting Interaction of IGF-II with the Insulin Receptor during Mouse Embryonic Development. Developmental Biology 1997, 189: 33-48. PMID: 9281335, DOI: 10.1006/dbio.1997.8666.Peer-Reviewed Original ResearchConceptsInsulin receptorGrowth-promoting interactionsMouse embryonic developmentMouse embryonic growthLigand/receptor interactionsGrowth-promoting functionEmbryonic day 13.5IGF-IINull mutantsTargeted MutagenesisGrowth of embryosMouse embryogenesisDouble mutantTriple mutantEmbryonic developmentGenetic evidenceIGF1R resultsOrgan hypoplasiaGenetic analysisMutantsEmbryonic growthType 1 IGF receptorDay 13.5Receptor interactionEmbryos
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