2013
Endothelial Cell–Dependent Regulation of Arteriogenesis
Moraes F, Paye J, Mac Gabhann F, Zhuang ZW, Zhang J, Lanahan AA, Simons M. Endothelial Cell–Dependent Regulation of Arteriogenesis. Circulation Research 2013, 113: 1076-1086. PMID: 23897694, PMCID: PMC3865810, DOI: 10.1161/circresaha.113.301340.Peer-Reviewed Original ResearchConceptsAdult arteriogenesisCell-autonomous fashionGrowth factor signalingMouse linesCell-autonomous effectsKnockin mouse lineMorphogenetic defectsArterial morphogenesisCell type-specific deletionFactor signalingCell typesCre-driver mouse linesSynectinAttractive therapeutic strategyOcclusive atherosclerotic diseaseMuscle cellsEndothelial cellsRegulationArterial conduitsAtherosclerotic diseaseTherapeutic strategiesAdult miceClinical importanceArteriogenesisCellsNO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth
Jaba IM, Zhuang ZW, Li N, Jiang Y, Martin KA, Sinusas AJ, Papademetris X, Simons M, Sessa WC, Young LH, Tirziu D. NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. Journal Of Clinical Investigation 2013, 123: 1718-1731. PMID: 23454748, PMCID: PMC3613910, DOI: 10.1172/jci65112.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, BiologicalAnimalsCell EnlargementCells, CulturedCoronary VesselsEndothelium, VascularHeart VentriclesMechanistic Target of Rapamycin Complex 1MiceMice, Inbred C57BLMice, TransgenicMultiprotein ComplexesMyocytes, CardiacNeovascularization, PhysiologicNG-Nitroarginine Methyl EsterNitric OxideNitric Oxide SynthasePlacenta Growth FactorPregnancy ProteinsProteinsProteolysisProto-Oncogene Proteins c-aktRatsRats, Sprague-DawleyRGS ProteinsSignal TransductionTOR Serine-Threonine KinasesConceptsCardiomyocyte growthAkt/mTORC1 signalingNovel NO-dependent mechanismProteasomal degradationCoordination of angiogenesisMTORC1 signalingConditional overexpressionMurine cardiac tissueG proteinsTransgenic expressionAkt/Physiological mechanismsMyocyte growthVessel growthGrowth factorTransgenic miceHypertrophic responseAngiogenesisKnockout miceMyocardial hypertrophyExpressionGrowthCardiac hypertrophyNOS inhibitor L-NAMEInduction
2011
FGF-dependent regulation of VEGF receptor 2 expression in mice
Murakami M, Nguyen LT, Hatanaka K, Schachterle W, Chen PY, Zhuang ZW, Black BL, Simons M. FGF-dependent regulation of VEGF receptor 2 expression in mice. Journal Of Clinical Investigation 2011, 121: 2668-2678. PMID: 21633168, PMCID: PMC3223828, DOI: 10.1172/jci44762.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedEndothelial CellsEnzyme ActivationFibroblast Growth FactorsHindlimbHumansIschemiaMiceMice, Inbred C57BLMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Neovascularization, PhysiologicProto-Oncogene Proteins c-etsSignal TransductionVascular Endothelial Growth Factor Receptor-2Vascular Endothelial Growth FactorsConceptsFGF-dependent regulationEts transcription factorsERK1/2-dependent mannerVEGF receptor-2 expressionActivation of ERK1/2Enhancer activationTranscriptional analysisTranscription factorsVEGFR2 expressionVascular morphogenesisFGF stimulationVEGF stimulationFGFVascular formationExpressionVascular integrityReceptor 2 expressionActivationMorphogenesisVEGFERK1/2Numerous studiesPathwayRegulationDownregulationChallenging the Surgical Rodent Hindlimb Ischemia Model with the Miniinterventional Technique
Zhuang ZW, Shi J, Rhodes JM, Tsapakos MJ, Simons M. Challenging the Surgical Rodent Hindlimb Ischemia Model with the Miniinterventional Technique. Journal Of Vascular And Interventional Radiology 2011, 22: 1437-1446. PMID: 21459613, PMCID: PMC3367666, DOI: 10.1016/j.jvir.2010.12.039.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood Flow VelocityBone Marrow TransplantationChemokinesCytokinesDisease Models, AnimalEmbolization, TherapeuticFemoral ArteryHindlimbInflammation MediatorsIschemiaLaser-Doppler FlowmetryLigationMaleMuscle, SkeletalNeovascularization, PhysiologicRatsRats, Inbred LewRecovery of FunctionRegional Blood FlowTime FactorsConceptsMarrow mononuclear cellsLaser Doppler imagingRight femoral arterySurgical ligationFemoral arteryHindlimb ischemia modelSL groupInterventional embolizationIschemia modelBone marrow mononuclear cellsGranulocyte colony-stimulating factorPreserves tissue integrityColony-stimulating factorVivo microscopic imagesHindlimb ischemic modelChemokine assaysMassive scarringInflammatory infiltrationIL-18Lewis ratsNerve degenerationIL-13IL-6Mononuclear cellsIschemic model
2010
ERK1/2-Akt1 crosstalk regulates arteriogenesis in mice and zebrafish
Ren B, Deng Y, Mukhopadhyay A, Lanahan AA, Zhuang ZW, Moodie KL, Mulligan-Kehoe MJ, Byzova TV, Peterson RT, Simons M. ERK1/2-Akt1 crosstalk regulates arteriogenesis in mice and zebrafish. Journal Of Clinical Investigation 2010, 120: 1217-1228. PMID: 20237411, PMCID: PMC2846043, DOI: 10.1172/jci39837.Peer-Reviewed Original ResearchMeSH KeywordsAdaptor Proteins, Signal TransducingAnimalsArteriesCarrier ProteinsMaleMiceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3MorphogenesisNeovascularization, PhysiologicNeuropeptidesPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionVascular Endothelial Growth Factor AVascular Endothelial Growth Factor Receptor-2ZebrafishZebrafish Proteins
2006
Arteriogenesis: Noninvasive Quantification with Multi–Detector Row CT Angiography and Three-dimensional Volume Rendering in Rodents
Zhuang ZW, Gao L, Murakami M, Pearlman JD, Sackett TJ, Simons M, de Muinck ED. Arteriogenesis: Noninvasive Quantification with Multi–Detector Row CT Angiography and Three-dimensional Volume Rendering in Rodents. Radiology 2006, 240: 698-707. PMID: 16926325, DOI: 10.1148/radiol.2403050976.Peer-Reviewed Original ResearchMeSH KeywordsAngiographyAnimalsArteriesImaging, Three-DimensionalNeovascularization, PhysiologicRatsRats, Inbred LewTomography, X-Ray ComputedConceptsBone marrow-derived mononuclear cellsTotal arterial areaBlood vessel areaCT angiographyNonischemic legsBMC groupArterial areaMarrow-derived mononuclear cellsTotal blood vessel areaMulti-detector row CT angiographyVessel areaRight femoral arteryUnpaired Student's t-testTotal blood vessel volumeBlood vessel densityMulti-detector rowArterial growthBlood vessel volumeStudent's t-testTwo-way analysisRow CT angiographyBMC injectionBarium sulfate suspensionLewis ratsMore arteries