2024
ZFYVE21 promotes endothelial nitric oxide signaling and vascular barrier function in the kidney during aging
Jiang Q, Song G, He L, Li X, Jiang B, Wang Q, Wang S, Kim C, Barkestani M, Lopez R, Fan M, Wanniarachchi K, Quaranta M, Tian X, Mani A, Gonzalez A, Goodwin J, Sessa W, Ishibe S, Jane-Wit D. ZFYVE21 promotes endothelial nitric oxide signaling and vascular barrier function in the kidney during aging. Kidney International 2024, 106: 419-432. PMID: 38797325, PMCID: PMC11343665, DOI: 10.1016/j.kint.2024.05.007.Peer-Reviewed Original ResearchVascular barrier functionEndothelial cellsReduced endothelial nitric oxideRegulator of vascular barrier functionEndothelial nitric oxide signalingENOS activityEndosome-associated proteinsBarrier functionKidney organ culturesEndothelial nitric oxideAkt-dependent mannerNitric oxide donorGTPase Rab5Nitric oxide signalingZFYVE21Live cell imagingKidney insufficiencyReporter miceTrafficking mechanismsAccelerated aging phenotypeKnockout miceInterstitial edemaKidney functionVesicular populationOxide donor
2020
Murine Epsins Play an Integral Role in Podocyte Function
Wang Y, Pedigo CE, Inoue K, Tian X, Cross E, Ebenezer K, Li W, Wang Z, Shin JW, Schwartze E, Groener M, Ishibe S. Murine Epsins Play an Integral Role in Podocyte Function. Journal Of The American Society Of Nephrology 2020, 31: 2870-2886. PMID: 33051360, PMCID: PMC7790213, DOI: 10.1681/asn.2020050691.Peer-Reviewed Original ResearchConceptsSerum response factorPodocyte-specific lossCell division control protein 42 homologCell adhesionRegulation of Cdc42Integrin expressionProtein Cdc42Membrane proteinsTriple knockout miceEpsinDownstream regulationFoot process effacementPodocyte functionResponse factorPrimary podocytesCdc42Essential roleIndispensable roleProcess effacementExpressionRegulationKnockout miceIntegral rolePodocytesHomolog
2017
Vinculin is required to maintain glomerular barrier integrity
Lausecker F, Tian X, Inoue K, Wang Z, Pedigo CE, Hassan H, Liu C, Zimmer M, Jinno S, Huckle AL, Hamidi H, Ross RS, Zent R, Ballestrem C, Lennon R, Ishibe S. Vinculin is required to maintain glomerular barrier integrity. Kidney International 2017, 93: 643-655. PMID: 29241625, PMCID: PMC5846847, DOI: 10.1016/j.kint.2017.09.021.Peer-Reviewed Original ResearchMeSH KeywordsAlbuminuriaAnimalsCell MovementCell Surface ExtensionsCells, CulturedFocal Adhesion Kinase 1Focal AdhesionsGlomerulonephritis, MembranousGlomerulosclerosis, Focal SegmentalMechanotransduction, CellularMice, Inbred C57BLMice, KnockoutNephrosis, LipoidPhosphorylationPodocytesVinculinZonula Occludens-1 ProteinConceptsIntegrin-mediated cell-matrix adhesionIntercellular junctionsRole of vinculinCell-matrix adhesionFocal adhesion sizeCell-matrix interactionsGlomerular filtration barrierPodocyte-specific knockout miceLocalization of vinculinFiltration barrierCytoplasmic proteinsAdhesion sizeCell protrusionsVinculinActin filamentsPodocyte foot processesCell migrationProtein zonula occludens-1Expression levelsPrimary podocytesMutant miceZonula occludens-1Knockout miceFoot process effacementDramatic differencesLoss of the podocyte glucocorticoid receptor exacerbates proteinuria after injury
Zhou H, Tian X, Tufro A, Moeckel G, Ishibe S, Goodwin J. Loss of the podocyte glucocorticoid receptor exacerbates proteinuria after injury. Scientific Reports 2017, 7: 9833. PMID: 28852159, PMCID: PMC5575043, DOI: 10.1038/s41598-017-10490-z.Peer-Reviewed Original ResearchConceptsKnockout miceGlucocorticoid receptorNephrotic syndromeSimilar renal functionMainstay of therapyReceptor knockout miceTreatment of proteinuriaFoot process effacementMechanism of actionImmunomodulatory therapyRenal functionGlomerular injuryProtein excretionKO miceCommon disorderNephrotoxic serumPodocyte injuryPodocyte-specific deletionMouse modelSlit diaphragm proteinsWild-type podocytesProcess effacementProteinuriaUnstimulated conditionsKnockout animals