2024
Recent developments, opportunities, and challenges in the study of mRNA pseudouridylation
Gilbert W. Recent developments, opportunities, and challenges in the study of mRNA pseudouridylation. RNA 2024, 30: rna.079975.124. PMID: 38531650, PMCID: PMC11019745, DOI: 10.1261/rna.079975.124.Peer-Reviewed Original Research
2020
Regulation and Function of RNA Pseudouridylation in Human Cells
Borchardt EK, Martinez NM, Gilbert WV. Regulation and Function of RNA Pseudouridylation in Human Cells. Annual Review Of Genetics 2020, 54: 1-28. PMID: 32870730, PMCID: PMC8007080, DOI: 10.1146/annurev-genet-112618-043830.Peer-Reviewed Original ResearchConceptsRNA pseudouridylationHuman cellsRNA-binding proteinDistinct RNA sequencesMRNA pseudouridylationPseudouridine synthasesMolecular functionsRNA metabolismPseudouridylationGene expressionRNA conformationRNA targetsProtein productionRNA sequencesMessenger RNADiverse classRNATherapeutic mRNAWidespread effectsDestabilizing interactionsCellsRecent advancesSplicingSynthasesPotential effects
2019
mRNA structure determines modification by pseudouridine synthase 1
Carlile TM, Martinez NM, Schaening C, Su A, Bell TA, Zinshteyn B, Gilbert WV. mRNA structure determines modification by pseudouridine synthase 1. Nature Chemical Biology 2019, 15: 966-974. PMID: 31477916, PMCID: PMC6764900, DOI: 10.1038/s41589-019-0353-z.Peer-Reviewed Original ResearchConceptsMRNA target recognitionMRNA pseudouridylationMRNA targetsPost-transcriptional RNA modificationsRational mutational analysisRNA-protein interactionsPseudouridine synthase 1High-throughput kinetic analysisRNA structural motifsPseudouridine synthasePseudouridine synthasesRNA pseudouridylationRNA modificationsPseudouridylationMRNA structureRNA-RNAMutational analysisGene expressionRNA structurePredominant mRNAStructural context informationSynthase 1Specific sequencesComputational predictionsStructural motifs
2018
Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast
Wang YJ, Vaidyanathan PP, Rojas-Duran MF, Udeshi ND, Bartoli KM, Carr SA, Gilbert WV. Lso2 is a conserved ribosome-bound protein required for translational recovery in yeast. PLOS Biology 2018, 16: e2005903. PMID: 30208026, PMCID: PMC6135351, DOI: 10.1371/journal.pbio.2005903.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCodon, InitiatorConserved SequenceGene Expression Regulation, FungalHeLa CellsHumansPeptide Chain Elongation, TranslationalPeptide Chain Termination, TranslationalProtein BiosynthesisRibosomal ProteinsRibosomesRNA, RibosomalRNA, TransferSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsRibosome-binding proteinsRibosome-associated proteinsCodon-specific changesCoiled-coil domainQuantitative mass spectrometryGTPase activation centerRibosome-binding activityMost tRNAsRibosome profilingGene regulationMost genesCellular homeostasisTranslation defectsRibosomal RNATranslational recoveryComplete complementStart codonStop codonLso2Protein synthesisOpen reading frame 2ProteinImportant functionsCodonYeast
2016
Messenger RNA modifications: Form, distribution, and function
Gilbert WV, Bell TA, Schaening C. Messenger RNA modifications: Form, distribution, and function. Science 2016, 352: 1408-1412. PMID: 27313037, PMCID: PMC5094196, DOI: 10.1126/science.aad8711.Peer-Reviewed Original ResearchConceptsStable noncoding RNAsMRNA target siteOrganismal phenotypesDevelopmental regulatorsNoncoding RNAsMRNA modificationMolecular consequencesMessenger RNAMolecular effectsRNATarget siteRecent technical advancesDistinct modificationsSparse modificationEpitranscriptomeSplicingMethyladenosineRegulatorSpeciesTechnical advancesModificationPhenotypeRegulationFunctionCells
2014
Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells
Carlile TM, Rojas-Duran MF, Zinshteyn B, Shin H, Bartoli KM, Gilbert WV. Pseudouridine profiling reveals regulated mRNA pseudouridylation in yeast and human cells. Nature 2014, 515: 143-146. PMID: 25192136, PMCID: PMC4224642, DOI: 10.1038/nature13802.Peer-Reviewed Original Research