Cellular translational enhancer elements that recruit eukaryotic initiation factor 3.
Koubek J, Kaur J, Bhandarkar S, Lewis C, Niederer R, Stanciu A, Aitken C, Gilbert W. Cellular translational enhancer elements that recruit eukaryotic initiation factor 3. RNA 2024, rna.080310.124. PMID: 39626887, DOI: 10.1261/rna.080310.124.Peer-Reviewed Original ResearchEukaryotic initiation factor 3Initiation factor 3Eukaryotic gene expressionTranslational enhancer elementMRNA-specific translationNative 5'-UTRFactor 3Initiation factor activityHigh-throughput approachRibosome recruitmentTranslation initiationRibosome densityBind mRNACellular mRNAsTranslational enhancerEnhancer elementsGrowing cellsGene expressionCentral playerFunctional relevanceRibosomeStress conditionsFactor activityAlternative pathwayMRNARluA is the major mRNA pseudouridine synthase in Escherichia coli
Schaening-Burgos C, LeBlanc H, Fagre C, Li G, Gilbert W. RluA is the major mRNA pseudouridine synthase in Escherichia coli. PLOS Genetics 2024, 20: e1011100. PMID: 39241085, PMCID: PMC11421799, DOI: 10.1371/journal.pgen.1011100.Peer-Reviewed Original ResearchConceptsPseudouridine synthasesHigh-confidence sitesMRNA-modifying enzymesE. coli mRNAsStructure probing dataIdentified target sitesTarget siteDiverse eukaryotesBacterial mRNAsRNA modificationsRluAEscherichia coliSecondary structureE. coliTRNAPseudouridineRRNAStructural motifsMRNAModification capacityRecognition elementsSynthaseRNASequenceEukaryotesRecent developments, opportunities, and challenges in the study of mRNA pseudouridylation
Gilbert W. Recent developments, opportunities, and challenges in the study of mRNA pseudouridylation. RNA 2024, 30: rna.079975.124. PMID: 38531650, PMCID: PMC11019745, DOI: 10.1261/rna.079975.124.Peer-Reviewed Original Research