2019
The Functionally Important N‑Terminal Half of Fission Yeast Mid1p Anillin Is Intrinsically Disordered and Undergoes Phase Separation
Chatterjee M, Pollard TD. The Functionally Important N‑Terminal Half of Fission Yeast Mid1p Anillin Is Intrinsically Disordered and Undergoes Phase Separation. Biochemistry 2019, 58: 3031-3041. PMID: 31243991, PMCID: PMC7336169, DOI: 10.1021/acs.biochem.9b00217.Peer-Reviewed Original ResearchConceptsN-terminal halfFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeC-terminal halfFull-length proteinSchizosaccharomyces pombeStructure prediction toolsPH domainScaffold proteinInsect cellsAnimal cellsContractile ringMyosin IIOrganizing centerAnillinMid1pProteinHydrodynamic measurementsPombeCytokinesisUndergoes phase separationCellsFungalPrediction tools
2016
Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast
Laplante C, Huang F, Tebbs IR, Bewersdorf J, Pollard TD. Molecular organization of cytokinesis nodes and contractile rings by super-resolution fluorescence microscopy of live fission yeast. Proceedings Of The National Academy Of Sciences Of The United States Of America 2016, 113: e5876-e5885. PMID: 27647921, PMCID: PMC5056082, DOI: 10.1073/pnas.1608252113.Peer-Reviewed Original ResearchConceptsFluorescence photoactivation localization microscopyContractile ringLive fission yeast cellsLive fission yeastFission yeast cellsCytokinetic contractile ringSuper-resolution fluorescence microscopyCytokinesis nodesPhotoactivation localization microscopyFission yeastFormin Cdc12pActin networkIQ motifPlasma membraneCytokinesisConstituent proteinsMyosin-II tailYeast cellsActin ringsActin strandsAssembly precursorsDiscrete structural unitsFluorescence microscopyMolecular organizationProtein
2015
Three Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring
Laplante C, Berro J, Karatekin E, Hernandez-Leyva A, Lee R, Pollard TD. Three Myosins Contribute Uniquely to the Assembly and Constriction of the Fission Yeast Cytokinetic Contractile Ring. Current Biology 2015, 25: 1955-1965. PMID: 26144970, PMCID: PMC4526439, DOI: 10.1016/j.cub.2015.06.018.Peer-Reviewed Original ResearchConceptsContractile ringActin filamentsHeavy chain geneMyosin IIContractile ring assemblyFission yeast cellsMyosin heavy chain geneCytokinetic contractile ringConventional myosin IICytokinetic nodesMyo2Myo51Myp2Ring assemblyYeast cellsMyosin functionCytokinesisDeletion mutationsComplete assemblyMyosin VConstriction rateGenesMutationsCellsFilaments
2007
Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast
Vavylonis D, Wu JQ, Hao S, O'Shaughnessy B, Pollard TD. Assembly Mechanism of the Contractile Ring for Cytokinesis by Fission Yeast. Science 2007, 319: 97-100. PMID: 18079366, DOI: 10.1126/science.1151086.Peer-Reviewed Original ResearchConceptsContractile ringActin filamentsLive fission yeast cellsIndividual daughter cellsFission yeast cellsDynamic actin filamentsFission yeastMotor protein myosinCell equatorDaughter cellsYeast cellsAssembly mechanismProtein myosinFluorescence microscopyCytokinesisMyosinFilamentsTransient connectionsCellsYeastFungiMechanismMeshwork