2022
Oncometabolites as Regulators of DNA Damage Response and Repair
Gueble SE, Bindra RS. Oncometabolites as Regulators of DNA Damage Response and Repair. Seminars In Radiation Oncology 2022, 32: 82-94. PMID: 34861999, DOI: 10.1016/j.semradonc.2021.09.004.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsDNA damage responseDamage responseDDR defectsMultiple DNA repair pathwaysSynthetic lethal interactionsDNA repair pathwaysRepair pathwaysLethal interactionsDiverse mechanismsOncometaboliteCancer therapyCertain cancersNew mechanismTherapeutic strategiesSmall intermediatesBiologyRegulatorPathwayMechanismRepairDDRMetabolismDysregulationDefectsResponse
2014
Hypoxic Stress Facilitates Acute Activation and Chronic Downregulation of Fanconi Anemia Proteins
Scanlon SE, Glazer PM. Hypoxic Stress Facilitates Acute Activation and Chronic Downregulation of Fanconi Anemia Proteins. Molecular Cancer Research 2014, 12: 1016-1028. PMID: 24688021, PMCID: PMC4101147, DOI: 10.1158/1541-7786.mcr-13-0628.Peer-Reviewed Original ResearchConceptsFanconi anemia proteinsDNA repair pathwaysFA pathwayGenomic instabilityRepair pathwaysUbiquitination of FANCD2Intact FA pathwayATR-dependent mannerFA pathway activationAccumulation of γH2AXGenome integrityReplication stressTranscriptional repressionHypoxic conditionsFANCD2Cell survivalDNA damageHypoxic downregulationProtein downregulationHypoxic stressFANCIPathway activationPathwayKey membersCritical role
2013
Oxidative Stress in Mammalian Cells Impinges on the Cysteines Redox State of Human XRCC3 Protein and on Its Cellular Localization
Girard P, Graindorge D, Smirnova V, Rigolet P, Francesconi S, Scanlon S, Sage E. Oxidative Stress in Mammalian Cells Impinges on the Cysteines Redox State of Human XRCC3 Protein and on Its Cellular Localization. PLOS ONE 2013, 8: e75751. PMID: 24116071, PMCID: PMC3793007, DOI: 10.1371/journal.pone.0075751.Peer-Reviewed Original ResearchConceptsHomologous recombinationSensitivity to camptothecinCysteine to serineInhibition of DNA replicationCysteine redox stateExposure to camptothecinCellular reducing systemsTargets of ROSIn silico predictionMutant proteinsRAD51 paralogsGenome stabilitySubcellular localizationNon-reducing SDS-PAGEDNA replicationIncreased electrophoretic mobilityMutated proteinChromatin fractionHR pathwayCamptothecin treatmentCysteine residuesInduce DNA damageHuman cellsCellular localizationCHO cells