2023
Female naïve human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations
Patterson B, Yang B, Tanaka Y, Kim K, Cakir B, Xiang Y, Kim J, Wang S, Park I. Female naïve human pluripotent stem cells carry X chromosomes with Xa-like and Xi-like folding conformations. Science Advances 2023, 9: eadf2245. PMID: 37540754, PMCID: PMC10403202, DOI: 10.1126/sciadv.adf2245.Peer-Reviewed Original ResearchConceptsNaïve human pluripotent stem cellsHuman pluripotent stem cellsX-chromosome inactivationX chromosomePluripotent stem cellsStem cellsNaïve human embryonic stem cellsX chromosome stateX chromosome statusInactive X chromosomeActive X chromosomeHuman embryonic stem cellsEarly embryonic cellsEmbryonic stem cellsUnique epigenetic regulationChromatin compactionGenomic resolutionEpigenetic regulationChromosome inactivationChromosome stateSomatic cellsEmbryonic cellsChromosomesChromosome statusCells
2021
TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations
Cheng Y, Liu M, Hu M, Wang S. TAD-like single-cell domain structures exist on both active and inactive X chromosomes and persist under epigenetic perturbations. Genome Biology 2021, 22: 309. PMID: 34749781, PMCID: PMC8574027, DOI: 10.1186/s13059-021-02523-8.Peer-Reviewed Original ResearchConceptsInactive X chromosomeActive X chromosomeMajor epigenetic componentsSingle-cell domainsX chromosomeEpigenetic componentsThree-dimensional genome architectureGlobal epigenetic landscapeFemale human cellsLoop extrusion mechanismSame genomic regionGenome architectureChromatin domainsTAD structureChromatin compactionEpigenetic landscapeTAD boundariesChromatin foldingGenomic regionsChromosome copiesGenomic techniquesEpigenetic perturbationsEpigenetic interactionsDistinct cell linesChromosomes
2020
Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos
Sawh AN, Shafer MER, Su JH, Zhuang X, Wang S, Mango SE. Lamina-Dependent Stretching and Unconventional Chromosome Compartments in Early C. elegans Embryos. Molecular Cell 2020, 78: 96-111.e6. PMID: 32105612, PMCID: PMC7263362, DOI: 10.1016/j.molcel.2020.02.006.Peer-Reviewed Original ResearchConceptsB compartmentsEmbryonic chromosomesChromosome compartmentsInactive compartmentsChromosome domainsNuclear laminaEntire autosomesChromosomesCompartment boundariesUnappreciated roleDiverse conformationsDevelopmental contextChromosome stretchingCompartmentsConformationAutosomesChromatinGastrulationEmbryogenesisVariable positioningLaminaEmbryos
2016
Spatial organization of chromatin domains and compartments in single chromosomes
Wang S, Su JH, Beliveau BJ, Bintu B, Moffitt JR, Wu CT, Zhuang X. Spatial organization of chromatin domains and compartments in single chromosomes. Science 2016, 353: 598-602. PMID: 27445307, PMCID: PMC4991974, DOI: 10.1126/science.aaf8084.Peer-Reviewed Original ResearchConceptsIndividual chromosomesChromatin domainsX chromosomeNumerous genomic regionsSpatial organizationInactive X chromosomeGenome functionChromatin organizationGenomic regionsFractal globule modelSingle chromosomePolarized mannerChromosomesDifferent foldingTADCompartmentsAutosomesChromatinDomainTADsFoldingRegulationCapture studies