Featured Publications
Integrin intra-heterodimer affinity inversely correlates with integrin activatability
Sun G, Guillon E, Holley SA. Integrin intra-heterodimer affinity inversely correlates with integrin activatability. Cell Reports 2021, 35: 109230. PMID: 34107244, PMCID: PMC8227800, DOI: 10.1016/j.celrep.2021.109230.Peer-Reviewed Original ResearchConceptsFluorescence cross-correlation spectroscopyCell surface expressionHeterodimeric cell surface receptorsPoor cell surface expressionCell surface stabilityRobust cell surface expressionExtracellular matrix proteinsCell surface receptorsZebrafish somitogenesisSurface expressionCross-correlation spectroscopyFluorescence resonance energy transferIntegrin activationΒ-subunitIntegrin heterodimersFibronectin matrixMatrix proteinsVivo contextConformational changesCell adhesionResonance energy transferSurface receptorsIntegrin αvβ1Biophysical measurementsIntegrinsFibronectin is a smart adhesive that both influences and responds to the mechanics of early spinal column development
Guillon E, Das D, Jülich D, Hassan AR, Geller H, Holley S. Fibronectin is a smart adhesive that both influences and responds to the mechanics of early spinal column development. ELife 2020, 9: e48964. PMID: 32228864, PMCID: PMC7108867, DOI: 10.7554/elife.48964.Peer-Reviewed Original ResearchConceptsNeural tubeParaxial mesodermZebrafish neural tubeNormal vertebrate developmentVertebrate trunkVertebrate developmentFibronectin remodelingFibronectin matrixConvergence defectsMorphogenesisExtracellular matrixMesodermFibronectinColumn developmentSmart adhesivesCadherinMutantsAdhesive lap jointsAdhesionLap jointsSymmetric interfacesRemodelingShear stressDefects
2024
Live imaging of Fibronectin 1a-mNeonGreen and Fibronectin 1b-mCherry knock-in alleles during early zebrafish development
Jülich D, Holley S. Live imaging of Fibronectin 1a-mNeonGreen and Fibronectin 1b-mCherry knock-in alleles during early zebrafish development. Cells And Development 2024, 177: 203900. PMID: 38218338, PMCID: PMC10947920, DOI: 10.1016/j.cdev.2024.203900.Peer-Reviewed Original ResearchKnock-in alleleECM fibersLoss of function phenotypesGenetic complementation experimentsAdhesion receptor familyComplementation experimentsDouble mutantLive in vivo imagingExtracellular matrix glycoproteinFibronectin matrixPosterior hindgutPresomitic mesodermZebrafish developmentExpression patternsMRNA injectionIntegrinLive imagingCross-regulationAllelesFibronectinReceptor familyParaxial mesodermCo-localizationExtracellular matrixMutants