2018
Analysis of Cell Viability by the Lactate Dehydrogenase Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the Lactate Dehydrogenase Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095497. PMID: 29858337, DOI: 10.1101/pdb.prot095497.Peer-Reviewed Original ResearchConceptsCytoplasmic enzymePlasma membraneConversion of lactateLactate dehydrogenase assayLactate dehydrogenaseCellular damageYellow tetrazolium saltCell viabilityAmount of LDHDehydrogenase assayEnzymeCellsNADHTetrazolium saltCell culture supernatantsCulture supernatantsLDH activityAmount of formazanApoptosisDehydrogenaseMembraneActivityAssaysAnalysis of Cell Viability by the MTT Assay.
Kumar P, Nagarajan A, Uchil PD. Analysis of Cell Viability by the MTT Assay. Cold Spring Harbor Protocols 2018, 2018: pdb.prot095505. PMID: 29858338, DOI: 10.1101/pdb.prot095505.Peer-Reviewed Original ResearchConceptsInsoluble purple formazanMitochondrial reductaseCell stressCell deathLive cellsDirect cell deathConversion of substrateMetabolic activityCell viabilityPurple formazanViability assaysNonadherent cellsPlate formatCellsAssaysMTT assayMTTChromogenic productSensitive assayReductaseToxic agentsPopular assayFormazanViabilityLarge changes
2017
Assay for β-Galactosidase in Extracts of Mammalian Cells.
Uchil PD, Nagarajan A, Kumar P. Assay for β-Galactosidase in Extracts of Mammalian Cells. Cold Spring Harbor Protocols 2017, 2017: pdb.prot095778. PMID: 28974657, DOI: 10.1101/pdb.prot095778.Peer-Reviewed Original Research