1999
In vitro transposition of Tn552: A tool for DNA sequencing and mutagenesis
Griffin T, Leschziner A, Grindley N, Parsons L, DeVost J, Derbyshire K. In vitro transposition of Tn552: A tool for DNA sequencing and mutagenesis. Nucleic Acids Research 1999, 27: 3859-3865. PMID: 10481025, PMCID: PMC148649, DOI: 10.1093/nar/27.19.3859.Peer-Reviewed Original ResearchConceptsDNA sequencingTarget DNAEntire operonInsertion mutantsGene clusterGenetic toolsNucleotide sequenceSequence specificityUnique sequencesMolecular biologyTransposition reactionNovel transposonTransposonSequencingComprehensive collectionDNAArsenal of toolsSequenceOperonGenomicsMutantsCosmidsMutagenesisKbBiology
1991
The 3′‐5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction.
Derbyshire V, Grindley N, Joyce C. The 3′‐5′ exonuclease of DNA polymerase I of Escherichia coli: contribution of each amino acid at the active site to the reaction. The EMBO Journal 1991, 10: 17-24. PMID: 1989882, PMCID: PMC452606, DOI: 10.1002/j.1460-2075.1991.tb07916.x.Peer-Reviewed Original ResearchConceptsActive siteMetal ionsEnzyme-bound metal ionSide chainsExonuclease reactionDivalent metal ionsAmino acid side chainsCarboxylate side chainAcid side chainsHydroxide ionMetal ligandsNucleophilic attackIonsTerminal phosphodiester bondPhosphodiester bondReactionExonuclease active siteActivity resultsKlenow fragmentDuplex DNA substratesCatalysisChainCarboxylateTerminal baseSubstrate