2024
Beyond Earth’s bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space
Chaplia O, Mathyk B, Nichols-Burns S, Basar M, Halicigil C. Beyond Earth’s bounds: navigating the frontiers of Assisted Reproductive Technologies (ART) in space. Reproductive Biology And Endocrinology 2024, 22: 123. PMID: 39394617, PMCID: PMC11468284, DOI: 10.1186/s12958-024-01290-y.Peer-Reviewed Original ResearchConceptsAssisted reproductive technologyIn vitro embryo developmentReproductive technologyRadiation-induced damageGamete handlingClinical embryologistsReproductive endocrinologistsTemperature limitEmbryo cultureReproductive medicineLow Earth orbitEmbryo developmentDeep space travelEarth orbitSpace environmentInterplanetary speciesEarth-boundMicrofluidic devicesCryopreservationMitochondrial Unfolded Protein Response Gene Clpp Is Required for Oocyte Function and Female Fertility
Ergun Y, Imamoglu A, Cozzolino M, Demirkiran C, Basar M, Garg A, Yildirim R, Seli E. Mitochondrial Unfolded Protein Response Gene Clpp Is Required for Oocyte Function and Female Fertility. International Journal Of Molecular Sciences 2024, 25: 1866. PMID: 38339144, PMCID: PMC10855406, DOI: 10.3390/ijms25031866.Peer-Reviewed Original ResearchConceptsCaseinolytic peptidase PMouse modelProtein homeostasisStress responseUnfolded protein stress responseProtein stress responseCumulus/granulosa cellsOocyte competenceOocyte functionGlobal deletionFunctional abnormalitiesGenes clpPMetabolic stress responseFemale subfertilityFemale infertilityOocyte-specificOocytesReproductive functionMtUPRMiceProtein degradationReproductive competenceFemale fertilityDeletionHomeostasis
2015
Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding
Kayisli O, Kayisli UA, Basar M, Semerci N, Schatz F, Lockwood CJ. Progestins Upregulate FKBP51 Expression in Human Endometrial Stromal Cells to Induce Functional Progesterone and Glucocorticoid Withdrawal: Implications for Contraceptive- Associated Abnormal Uterine Bleeding. PLOS ONE 2015, 10: e0137855. PMID: 26436918, PMCID: PMC4593551, DOI: 10.1371/journal.pone.0137855.Peer-Reviewed Original ResearchConceptsHuman endometrial stromal cellsAbnormal uterine bleedingEndometrial stromal cellsMedroxyprogesterone acetateGuinea pigsUterine bleedingCultured human endometrial stromal cellsStromal cellsGlandular cellsProgestin only contraceptivesEndometrial blood flowOvariectomized guinea pigsMajor side effectsEffective family planning methodsGlucocorticoid receptor activationGR transcriptional activityFragile blood vesselsFamily planning methodsIngenuity Pathway AnalysisFKBP51 expressionFunctional withdrawalMicroarray resultsOnly contraceptivesGlucocorticoid withdrawalEndometrial inflammationLong-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival
Kayisli UA, Basar M, Guzeloglu-Kayisli O, Semerci N, Atkinson HC, Shapiro J, Summerfield T, Huang SJ, Prelle K, Schatz F, Lockwood CJ. Long-acting progestin-only contraceptives impair endometrial vasculature by inhibiting uterine vascular smooth muscle cell survival. Proceedings Of The National Academy Of Sciences Of The United States Of America 2015, 112: 5153-5158. PMID: 25847994, PMCID: PMC4413279, DOI: 10.1073/pnas.1424814112.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell CountCell DifferentiationCell MovementCell ProliferationCell SurvivalCells, CulturedChemokine CCL2Contraceptive Agents, FemaleDesogestrelEndometriumFemaleGene Expression ProfilingGene Expression RegulationGuinea PigsHumansMedroxyprogesterone AcetateModels, BiologicalMuscle, Smooth, VascularMyocytes, Smooth MuscleOvariectomyProgestinsProliferating Cell Nuclear AntigenSignal TransductionSTAT1 Transcription FactorConceptsVascular smooth muscle cellsChemokine ligand 2Medroxyprogesterone acetateEndometrial vasculatureVSMC proliferationCultured vascular smooth muscle cellsSmooth muscle cellsSmooth muscle cell survivalVascular smooth muscle cell survivalCell nuclear antigenFragile microvesselsΑSMA immunoreactivityEndometrial vesselsΑSMA stainingPericyte proliferationSTAT1 inhibitorGuinea pigsMuscle cellsMuscle cell survivalCCL2 effectsEtonogestrelMigration assaysNuclear antigenVSMC differentiationWomenChinese herbal medicine for miscarriage affects decidual micro-environment and fetal growth
Piao L, Chen C, Yeh C, Basar M, Masch R, Cheng Y, Lockwood CJ, Schatz F, Huang SJ. Chinese herbal medicine for miscarriage affects decidual micro-environment and fetal growth. Placenta 2015, 36: 559-566. PMID: 25771406, PMCID: PMC4395550, DOI: 10.1016/j.placenta.2015.02.006.Peer-Reviewed Original ResearchConceptsFirst trimester decidual cellsIL-1βDecidual cellsPlacental weightFetal growthFood intakeResorption rateFetal resorption rateM-CSFMaternal food intakeC motif ligand 2Intrauterine growth restrictionIGF-IR expressionInducible protein-10Anti-CD163 antibodyQRT-PCRChinese herbal formulaIL-8 productionChinese herbal medicineG-CSF expressionEndothelial cell angiogenesisPlacental IGFDecidual macrophagesFetal weightCytokine expression
2014
Unfolded protein response prevents blastocyst formation during preimplantation embryo development in vitro
Basar M, Bozkurt I, Guzeloglu-Kayisli O, Sozen B, Tekmen I, Schatz F, Arici A, Lockwood CJ, Kayisli UA. Unfolded protein response prevents blastocyst formation during preimplantation embryo development in vitro. Fertility And Sterility 2014, 102: 1777-1784. PMID: 25305729, DOI: 10.1016/j.fertnstert.2014.09.004.Peer-Reviewed Original ResearchConceptsUnfolded protein responseProtein responseBlastocyst formationEmbryo developmentActivation of UPRPreimplantation embryo developmentTM treatmentBlastocyst developmentEffect of tunicamycinEndoplasmic reticulum stressEmbryonic developmentER stressCell embryosNuclear fragmentationMorphological differencesImmunoglobulin proteinBlastocyst stageReticulum stressTunicamycinDevelopment ratePregnant mare serum gonadotropinBlastocyst development rateProtein-1 mRNA expressionNongenomic mechanismsEmbryosHypertension alters phosphorylation of VASP in brain endothelial cells
Arlier Z, Basar M, Kocamaz E, Kiraz K, Tanriover G, Kocer G, Arlier S, Giray S, Nasırcılar S, Gunduz F, Senturk U, Demir N. Hypertension alters phosphorylation of VASP in brain endothelial cells. International Journal Of Neuroscience 2014, 125: 288-297. PMID: 24894047, DOI: 10.3109/00207454.2014.930740.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood PressureBrainCase-Control StudiesCell Adhesion MoleculesCells, CulturedDisease Models, AnimalEndothelial CellsExercise TherapyGene Expression RegulationHumansHypertensionHypoxiaMicrofilament ProteinsOxygenPhosphoproteinsPhosphorylationRatsRats, Inbred SHRRats, Inbred WKYSerineStatistics, NonparametricSwimmingTime FactorsConceptsBrain endothelial cellsVASP expressionEndothelial cellsVasodilator-stimulated phosphoproteinVASP phosphorylationWestern blottingHypertension-induced alterationsIndirect salutary effectsEffects of hypertensionEffects of exerciseNormoxic endothelial cellsEndothelial barrier functionWKY ratsEndothelial changesTissue hypoxiaCerebral vasculatureHypertensionSalutary effectsExercise conditionsBarrier functionVascular systemExerciseAlters phosphorylationImmunocytochemistryBlottingThe comparative effects of perindopril and catechin on mesangial matrix and podocytes in the streptozotocin induced diabetic rats
Ertürküner S, Başar M, Tunçdemir M, Seçkin İ. The comparative effects of perindopril and catechin on mesangial matrix and podocytes in the streptozotocin induced diabetic rats. Pharmacological Reports 2014, 66: 279-287. PMID: 24911082, DOI: 10.1016/j.pharep.2013.09.010.Peer-Reviewed Original ResearchConceptsUntreated diabetic groupDiabetic groupMesangial matrixControl groupDiabetic nephropathyDiabetes mellitusEnd-stage kidney failureOxidative stressExperimental diabetic nephropathyHigh blood pressureAdult Wistar AlbinoGlomerular mesangial matrixBlood glucose valuesExcessive reactive oxygen species (ROS) productionReactive oxygen species productionBlood pressureKidney weightDiabetic ratsKidney failureImmunohistochemical stainingOxygen species productionPodocyte damageBody weightEnzyme inhibitorsWistar albino
2012
Statins Inhibit Monocyte Chemotactic Protein 1 Expression in Endometriosis
Cakmak H, Basar M, Seval-Celik Y, Osteen KG, Duleba AJ, Taylor HS, Lockwood CJ, Arici A. Statins Inhibit Monocyte Chemotactic Protein 1 Expression in Endometriosis. Reproductive Sciences 2012, 19: 572-579. PMID: 22267540, PMCID: PMC3439122, DOI: 10.1177/1933719111430998.Peer-Reviewed Original ResearchConceptsCultured endometriotic cellsMonocyte chemotactic protein-1 expressionChemotactic protein-1 expressionMCP-1 productionEndometriotic cellsProtein-1 expressionEndometriotic implantsMouse modelMCP-1 messenger RNA expressionExerts anti-inflammatory effectsAnti-inflammatory effectsEffect of statinsAnti-inflammatory propertiesMCP-1 expressionMevalonate pathwayDose-dependent inhibitionNude mouse modelMessenger RNA expressionDose-dependent mannerEctopic endometriumEndometrial cellsPeritoneal surfaceStatinsPotential treatmentInhibitory effect
2010
The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia
Huang SJ, Zenclussen AC, Chen CP, Basar M, Yang H, Arcuri F, Li M, Kocamaz E, Buchwalder L, Rahman M, Kayisli U, Schatz F, Toti P, Lockwood CJ. The Implication of Aberrant GM-CSF Expression in Decidual Cells in the Pathogenesis of Preeclampsia. American Journal Of Pathology 2010, 177: 2472-2482. PMID: 20829438, PMCID: PMC2966804, DOI: 10.2353/ajpath.2010.091247.Peer-Reviewed Original ResearchConceptsGranulocyte-macrophage colony-stimulating factorColony-stimulating factorPathogenesis of preeclampsiaDendritic cellsDecidual cellsFirst trimester human decidual cellsGranulocyte-macrophage colony-stimulating factor (GM-CSF) expressionGenesis of preeclampsiaSystemic inflammatory stateInterleukin-1 βTumor necrosis factorHuman decidual cellsColony-stimulating factor expressionFactor expression levelsColony-stimulating factor productionGM-CSF expressionGranulocyte-macrophage colony-stimulating factor productionPreeclamptic deciduaShallow placentationInflammatory stateHuman deciduaNecrosis factorMurine modelPreeclampsiaMouse model
2007
Basic aspects of implantation
Guzeloglu-Kayisli O, Basar M, Arici A. Basic aspects of implantation. Reproductive BioMedicine Online 2007, 15: 728-739. PMID: 18062873, DOI: 10.1016/s1472-6483(10)60541-x.Peer-Reviewed Original ResearchConceptsSuccessful implantationPlacental formationGp130 cytokine familyIL-15 systemNumber of cytokinesGrowth factor-beta superfamilyColony-Stimulating FactorLeukemia inhibitory factorProcess of implantationSynchronized dialogueUterine factorsReceptive endometriumUterine growthIL-1Sex steroidsEmbryo implantationBlastocyst adhesionInhibitory factorCytokine familyImplantation sitesGrowth factorJuxtacrine mannerImplantationSpecific cohortCytokines