2024
Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model
Sakamoto R, Murrell M. Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model. Nature Communications 2024, 15: 9731. PMID: 39523366, PMCID: PMC11551154, DOI: 10.1038/s41467-024-53228-y.Peer-Reviewed Original ResearchConceptsMyosin-induced stressContractile ring assemblyCell division mechanismActin filamentsActin cortexCleavage furrowDivision planeActomyosin flowsGiant unilamellar vesiclesRing assemblyCell divisionMyosin activityContractile ring-like structureShape changesRing-like structureDivision mechanismEnergetic costSymmetric divisionActinRing-like formationCell modelUnilamellar vesiclesIn vitro modelFurrowCellsComposite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model
Sakamoto R, Murrell M. Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model. Communications Biology 2024, 7: 840. PMID: 38987288, PMCID: PMC11236970, DOI: 10.1038/s42003-024-06528-4.Peer-Reviewed Original ResearchConceptsF-actin networkF-actinF-actin architectureMembrane shape changesCell shape changesActivity of myosinInduce membrane deformationActomyosin contractilityShape changesActin cortexActomyosin cortexGiant unilamellar vesiclesActinMembrane deformationUnilamellar vesiclesCell modelNo-slip boundariesForce generationActomyosinMyosinVesiclesForce transmission
2022
In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles.
Chen S, Sun Z, Murrell M. In Vitro Reconstitution of the Actin Cytoskeleton Inside Giant Unilamellar Vesicles. Journal Of Visualized Experiments 2022 PMID: 36094272, DOI: 10.3791/64026.Peer-Reviewed Original ResearchConceptsGiant unilamellar vesiclesCytoskeleton networkLipid dropletsCell‐mimicking systemUnilamellar vesiclesActin cytoskeletonVitro reconstitutionGenetic regulationActin networkBiochemical regulationSynthetic biologyCellular activitiesLive cellsMixture of proteinsActin polymersLipid componentsVesiclesRegulationReconstitutionCellsCytoskeletonCell deformationMachineryBiologyQuantitative insights