2024
Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model
Sakamoto R, Murrell M. Mechanical power is maximized during contractile ring-like formation in a biomimetic dividing cell model. Nature Communications 2024, 15: 9731. PMID: 39523366, PMCID: PMC11551154, DOI: 10.1038/s41467-024-53228-y.Peer-Reviewed Original ResearchConceptsMyosin-induced stressContractile ring assemblyCell division mechanismActin filamentsActin cortexCleavage furrowDivision planeActomyosin flowsGiant unilamellar vesiclesRing assemblyCell divisionMyosin activityContractile ring-like structureShape changesRing-like structureDivision mechanismEnergetic costSymmetric divisionActinRing-like formationCell modelUnilamellar vesiclesIn vitro modelFurrowCellsEnergy partitioning in the cell cortex
Chen S, Seara D, Michaud A, Kim S, Bement W, Murrell M. Energy partitioning in the cell cortex. Nature Physics 2024, 20: 1824-1832. DOI: 10.1038/s41567-024-02626-6.Peer-Reviewed Original ResearchCell cortexEntropy production rateGAP expressionCortical actin filamentsRho GTPase pathwayGTPase pathwayMyosin IIActin filamentsDiversity patternsEnergy partitioningRhoOnsager reciprocityCell phenotypeProtein expressionThermodynamic equilibriumCellsSpiral travelling waveProduction rateTemporal dynamicsLiving systemsActinEnergyWavePhenotypeActivityComposite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model
Sakamoto R, Murrell M. Composite branched and linear F-actin maximize myosin-induced membrane shape changes in a biomimetic cell model. Communications Biology 2024, 7: 840. PMID: 38987288, PMCID: PMC11236970, DOI: 10.1038/s42003-024-06528-4.Peer-Reviewed Original ResearchConceptsF-actin networkF-actinF-actin architectureMembrane shape changesCell shape changesActivity of myosinInduce membrane deformationActomyosin contractilityShape changesActin cortexActomyosin cortexGiant unilamellar vesiclesActinMembrane deformationUnilamellar vesiclesCell modelNo-slip boundariesForce generationActomyosinMyosinVesiclesForce transmissionActive tension and membrane friction mediate cortical flows and blebbing in a model actomyosin cortex
Sakamoto R, Murrell M. Active tension and membrane friction mediate cortical flows and blebbing in a model actomyosin cortex. Physical Review Research 2024, 6: 033024. DOI: 10.1103/physrevresearch.6.033024.Peer-Reviewed Original ResearchActomyosin cortexCell membraneActin cytoskeletonCortical flowMembrane blebbingCell divisionCell migrationCytoskeletonActomyosinBiological phenomenaMembrane bulgesBlebsCellsMembraneViscoelastic fluidMechanical responseElastic stressesStress yieldActinUbiquitous structuresApoptosisMechanical stressMembrane elasticityPhysical behaviorGrowth‐induced collective bending and kinetic trapping of cytoskeletal filaments
Banerjee D, Freedman S, Murrell M, Banerjee S. Growth‐induced collective bending and kinetic trapping of cytoskeletal filaments. Cytoskeleton 2024, 81: 409-419. PMID: 38775207, DOI: 10.1002/cm.21877.Peer-Reviewed Original ResearchActin networkFilamentous growthActin filamentsTurnover of actin filamentsActin filament growthKinetic trapsActin poolFilament polymerizationActin cortexCytoskeletal filamentsSubunit poolActinFilamentsSubunitConsequence of growthFilament mechanismNematic defectsCrowded environmentLong-livedGrowthPoolAbundanceBending patternCellsTurnoverF-actin architecture determines the conversion of chemical energy into mechanical work
Sakamoto R, Murrell M. F-actin architecture determines the conversion of chemical energy into mechanical work. Nature Communications 2024, 15: 3444. PMID: 38658549, PMCID: PMC11043346, DOI: 10.1038/s41467-024-47593-x.Peer-Reviewed Original ResearchConceptsF-actin architectureF-actinATP consumption rateF-actin bundlesIn vitro reconstitutionDynamic cellular processesHigher ATP consumptionActin cytoskeletonFilamentous actinMyosin motorsCellular processesATP hydrolysisPurified componentsAdenosine triphosphateForce generationConversion of chemical energyATP consumptionConsumption rateActinChemical energyMyosinNetwork contractionCytoskeletonEnergetic principlesHydrolysis
2023
Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex
Sakamoto R, Banerjee D, Yadav V, Chen S, Gardel M, Sykes C, Banerjee S, Murrell M. Membrane tension induces F-actin reorganization and flow in a biomimetic model cortex. Communications Biology 2023, 6: 325. PMID: 36973388, PMCID: PMC10043271, DOI: 10.1038/s42003-023-04684-7.Peer-Reviewed Original ResearchConceptsF-actin reorganizationMechanical stressCell cortexBiochemical regulationCell shapeActomyosin cortexPhysical behaviorCell polarizationMembrane tensionCell migrationEssential physical behaviorPore openingSpatial assemblyDrive changesMembraneRelative rolesStressActinRegulationDiverse behaviorsRoleAssembly