2017
Panel 4: Report of the Microbiology Panel
Barenkamp SJ, Chonmaitree T, Hakansson AP, Heikkinen T, King S, Nokso‐Koivisto J, Novotny LA, Patel JA, Pettigrew M, Swords WE. Panel 4: Report of the Microbiology Panel. Otolaryngology 2017, 156: s51-s62. PMID: 28372529, PMCID: PMC5490388, DOI: 10.1177/0194599816639028.Peer-Reviewed Original ResearchConceptsOtitis mediaUpper respiratory tract infectionRespiratory tract infectionsAcute otitis mediaOtitis media casesRespiratory syncytial virusFuture treatment optionsTract infectionsBacterial coinfectionBacterial virulence determinantsBasic laboratory studiesSyncytial virusTreatment optionsAntibiotic treatmentEpidemiology of diseaseAnimal modelsPanel membersClinical practicePubMed databaseNational HarborVirulence determinantsChildrenInitial reviewBacteriologyDraft reviewsPanel 6: Vaccines
Pettigrew MM, Alderson MR, Bakaletz LO, Barenkamp SJ, Hakansson AP, Mason KM, Nokso‐Koivisto J, Patel J, Pelton SI, Murphy TF. Panel 6: Vaccines. Otolaryngology 2017, 156: s76-s87. PMID: 28372533, PMCID: PMC5505493, DOI: 10.1177/0194599816632178.Peer-Reviewed Original ResearchConceptsPneumococcal otitis mediaOtitis mediaVaccine antigensVaccine developmentH influenzaePneumococcal serotypesHaemophilus influenzae protein DSignificant health care burdenAdditional pneumococcal serotypesCorrelates of protectionHealth care burdenRecurrent otitis mediaEffectiveness of vaccinesTympanostomy tube insertionProtein-based vaccinesCandidate vaccine antigensOM pathogensOM visitsPCV effectivenessPneumococcal vaccineRespiratory virusesVaccine protectionTube insertionMoraxella catarrhalisCare burden
2016
Density, Serotype Diversity, and Fitness of Streptococcus pneumoniae in Upper Respiratory Tract Cocolonization With Nontypeable Haemophilus influenzae
Lewnard JA, Huppert A, Givon-Lavi N, Pettigrew MM, Regev-Yochay G, Dagan R, Weinberger DM. Density, Serotype Diversity, and Fitness of Streptococcus pneumoniae in Upper Respiratory Tract Cocolonization With Nontypeable Haemophilus influenzae. The Journal Of Infectious Diseases 2016, 214: 1411-1420. PMID: 27540112, PMCID: PMC5079371, DOI: 10.1093/infdis/jiw381.Peer-Reviewed Original ResearchConceptsNontypeable Haemophilus influenzaeNTHi colonizationPneumococcal serotypesChildren 2Haemophilus influenzaeStreptococcus pneumoniaeUpper respiratory tract carriageSerotype diversityComplex otitis mediaNasopharyngeal pneumococcal carriagePneumococcal conjugate vaccineImmune-evasive phenotypePneumococcal serotype diversityNTHi carriageConjugate vaccinePneumococcal carriageOtitis mediaStudy visitNeutrophil resistanceCapsular thicknessLogistic regressionDensity associationsCarriageSerotypesColonization density
2013
Panel 5: Microbiology and Immunology Panel
Murphy TF, Chonmaitree T, Barenkamp S, Kyd J, Nokso‐Koivisto J, Patel JA, Heikkinen T, Yamanaka N, Ogra P, Swords WE, Sih T, Pettigrew MM. Panel 5: Microbiology and Immunology Panel. Otolaryngology 2013, 148: e64-e89. PMID: 23536533, PMCID: PMC4021594, DOI: 10.1177/0194599812459636.Peer-Reviewed Original ResearchConceptsOtitis mediaPanel 6: Vaccines
Pelton SI, Pettigrew MM, Barenkamp SJ, Godfroid F, Grijalva CG, Leach A, Patel J, Murphy TF, Selak S, Bakaletz LO. Panel 6: Vaccines. Otolaryngology 2013, 148: e90-e101. PMID: 23536534, PMCID: PMC4029613, DOI: 10.1177/0194599812466535.Peer-Reviewed Original ResearchConceptsAcute otitis mediaPneumococcal conjugate vaccineOtitis mediaDevelopment of correlatesCandidate antigensAnimal modelsClinical efficacy trialsEffectiveness of vaccinesBurden of diseaseNontypeable Haemophilus influenzaeHuman clinical trialsOtitis pathogensConjugate vaccineVaccine strategiesPneumococcal serotypesClinical trialsMoraxella catarrhalisAdditional serotypesNew adjuvantsEfficacy trialsObservational studyFrequent recurrenceChild healthVaccine antigensMore antigens
2012
Bacterial and viral interactions within the nasopharynx contribute to the risk of acute otitis media
Ruohola A, Pettigrew MM, Lindholm L, Jalava J, Räisänen KS, Vainionpää R, Waris M, Tähtinen PA, Laine MK, Lahti E, Ruuskanen O, Huovinen P. Bacterial and viral interactions within the nasopharynx contribute to the risk of acute otitis media. Journal Of Infection 2012, 66: 247-254. PMID: 23266462, PMCID: PMC3571106, DOI: 10.1016/j.jinf.2012.12.002.Peer-Reviewed Original ResearchConceptsRisk of AOMAcute otitis mediaMiddle ear effusionAOM groupRespiratory virusesAOM pathogensAcute symptomsOtitis mediaEar effusionM. catarrhalisAcute inflammatory signsNasopharyngeal bacterial colonizationRespiratory tract infectionsOtoscopic signsTract infectionsInflammatory signsNasopharyngeal samplesSymptomatic childrenNasopharyngeal bacteriaH. influenzaePneumatic otoscopyS. pneumoniaeViral interactionsBacterial colonizationEffusionUpper Respiratory Tract Microbial Communities, Acute Otitis Media Pathogens, and Antibiotic Use in Healthy and Sick Children
Pettigrew MM, Laufer AS, Gent JF, Kong Y, Fennie KP, Metlay JP. Upper Respiratory Tract Microbial Communities, Acute Otitis Media Pathogens, and Antibiotic Use in Healthy and Sick Children. Applied And Environmental Microbiology 2012, 78: 6262-6270. PMID: 22752171, PMCID: PMC3416608, DOI: 10.1128/aem.01051-12.Peer-Reviewed Original ResearchConceptsAcute otitis mediaAntibiotic useS. pneumoniaeHealthy childrenM. catarrhalisH. influenzaeAcute otitis media pathogensConcurrent acute otitis mediaUpper respiratory tract infectionRespiratory tract infectionsOtitis media pathogensUpper respiratory tractCross-sectional studyYears of ageAOM pathogensTract infectionsOtitis mediaMoraxella catarrhalisRespiratory tractReal-time PCRNasal swabsSick childrenPrevention strategiesHaemophilus influenzaeStreptococcus pneumoniaeVirulence of Streptococcus pneumoniae serotype 6C in experimental otitis media
Sabharwal V, Figueira M, Pelton SI, Pettigrew MM. Virulence of Streptococcus pneumoniae serotype 6C in experimental otitis media. Microbes And Infection 2012, 14: 712-718. PMID: 22414497, PMCID: PMC3382049, DOI: 10.1016/j.micinf.2012.02.008.Peer-Reviewed Original ResearchConceptsAcute otitis mediaExperimental otitis mediaPneumococcal conjugate vaccineOtitis mediaSerotype 6CInvasive pneumococcal diseaseOverall carriage rateLow-density infectionsMiddle ear infectionStreptococcus pneumoniae serotype 6CReplacement serotypesPneumococcal diseaseVaccine serotypesConjugate vaccineNonvaccine serotypesCarriage rateEar infectionsNasopharyngeal colonizationChinchilla modelStreptococcus pneumoniaeHost defenseSerotypesDisease potentialInfectionDisease
2011
Viral-Bacterial Interactions and Risk of Acute Otitis Media Complicating Upper Respiratory Tract Infection
Pettigrew MM, Gent JF, Pyles RB, Miller AL, Nokso-Koivisto J, Chonmaitree T. Viral-Bacterial Interactions and Risk of Acute Otitis Media Complicating Upper Respiratory Tract Infection. Journal Of Clinical Microbiology 2011, 49: 3750-3755. PMID: 21900518, PMCID: PMC3209086, DOI: 10.1128/jcm.01186-11.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesComorbidityHaemophilus influenzaeHuman bocavirusHumansInfantMetapneumovirusMicrobial InteractionsMoraxella catarrhalisOtitis MediaPolymerase Chain ReactionProspective StudiesRespiratory Syncytial VirusesRespiratory Tract InfectionsRisk AssessmentStreptococcus pneumoniaeConceptsUpper respiratory tract infectionAcute otitis mediaRespiratory tract infectionsRespiratory syncytial virusOtitis mediaOtitis media riskTract infectionsViral loadSyncytial virusHaemophilus influenzaeStreptococcus pneumoniaeRespiratory Syncytial Virus LoadMedium-risk factorsRepeated-measures logistic regressionRSV viral loadViral-bacterial interactionsHigh viral loadSpecific virusesBacterial pathogensChildren ages 6Common complicationRespiratory virusesMedium riskProspective studyHuman bocavirusMicrobial Communities of the Upper Respiratory Tract and Otitis Media in Children
Laufer AS, Metlay JP, Gent JF, Fennie KP, Kong Y, Pettigrew MM. Microbial Communities of the Upper Respiratory Tract and Otitis Media in Children. MBio 2011, 2: 10.1128/mbio.00245-10. PMID: 21285435, PMCID: PMC3031303, DOI: 10.1128/mbio.00245-10.Peer-Reviewed Original ResearchConceptsOtitis mediaUpper respiratory tractS. pneumoniaeRespiratory tractOtitis media treatmentRespiratory virus seasonS. pneumoniae colonizationOtitis media pathogensCross-sectional studyOtitis media susceptibilityPneumoniae colonizationDecreased riskPneumococcal colonizationNasal swabsFrequent causePrevention strategiesStreptococcus pneumoniaeDemographic dataProtective floraPneumoniaeSwabsChildrenCausal pathwaysTractRisk
2010
Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine
Laufer AS, Thomas JC, Figueira M, Gent JF, Pelton SI, Pettigrew MM. Capacity of serotype 19A and 15B/C Streptococcus pneumoniae isolates for experimental otitis media: Implications for the conjugate vaccine. Vaccine 2010, 28: 2450-2457. PMID: 20067753, PMCID: PMC2851619, DOI: 10.1016/j.vaccine.2009.12.078.Peer-Reviewed Original ResearchConceptsSerotype 19ASerotype 15B/CChinchilla otitis media modelMiddle ear infectionOtitis media modelDisease prevention effortsStreptococcus pneumoniae isolatesExperimental otitis mediaStreptococcus pneumoniae serotypesQuantitative PCRConjugate vaccineOtitis mediaEar infectionsPneumoniae isolatesVaccine formulationsPneumoniae serotypesSame sequence typeVaccine targetsPrevention effortsPathogenic potentialDifferent serotypesSequence typesSerotypesIsolatesVaccine
2009
Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens
Thomas JC, Pettigrew MM. Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens. Methods In Molecular Biology 2009, 493: 179-190. PMID: 18839348, PMCID: PMC3581352, DOI: 10.1007/978-1-59745-523-7_11.Peer-Reviewed Original ResearchConceptsOtitis mediaMolecular epidemiologyMultilocus sequence typingMoraxella catarrhalisHaemophilus influenzaeStreptococcus pneumoniaeSequence typingPulsed-Field Gel ElectrophoresisEpidemiologyBacterial pathogensField gel electrophoresisType of studyResearch settingsCharacterization of isolatesPathogensCatarrhalisInfluenzaePneumoniae
2008
Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC
Pettigrew MM, Gent JF, Revai K, Patel JA, Chonmaitree T. Microbial Interactions during Upper Respiratory Tract Infections - Volume 14, Number 10—October 2008 - Emerging Infectious Diseases journal - CDC. Emerging Infectious Diseases 2008, 14: 1584-1591. PMID: 18826823, PMCID: PMC2609881, DOI: 10.3201/eid1410.080119.Peer-Reviewed Original ResearchMeSH KeywordsChild, PreschoolCohort StudiesFemaleHaemophilus InfectionsHaemophilus influenzaeHumansInfantMaleMoraxella catarrhalisMoraxellaceae InfectionsNasopharynxOtitis MediaProspective StudiesRespiratory Tract InfectionsStaphylococcal InfectionsStaphylococcus aureusStreptococcus pneumoniaeSuperinfectionConceptsUpper respiratory tract infectionH. influenzaeInfectious Diseases journal - CDCRespiratory tract infectionsS. pneumoniae colonizationHealthy children 6Months of ageS. aureusProspective cohortTract infectionsPneumoniae colonizationVaccination strategiesAntimicrobial therapyMoraxella catarrhalisChildren 6M. catarrhalisS. pneumoniaeHaemophilus influenzaeStreptococcus pneumoniaeBacterial infectionsSpecific bacterial speciesInfluenzaeLikelihood of colonizationCatarrhalisStaphylococcus aureus
2007
Genetic Diversity of Paired Middle-Ear and Pharyngeal Nontypeable Haemophilus influenzae Isolates from Children with Acute Otitis Media
Berrens ZJ, Marrs CF, Pettigrew MM, Sandstedt SA, Patel M, Gilsdorf JR. Genetic Diversity of Paired Middle-Ear and Pharyngeal Nontypeable Haemophilus influenzae Isolates from Children with Acute Otitis Media. Journal Of Clinical Microbiology 2007, 45: 3764-3767. PMID: 17804648, PMCID: PMC2168485, DOI: 10.1128/jcm.00964-07.Peer-Reviewed Original Research
2006
Association of surfactant protein A polymorphisms with otitis media in infants at risk for asthma
Pettigrew MM, Gent JF, Zhu Y, Triche EW, Belanger KD, Holford TR, Bracken MB, Leaderer BP. Association of surfactant protein A polymorphisms with otitis media in infants at risk for asthma. BMC Medical Genomics 2006, 7: 68. PMID: 16884531, PMCID: PMC1557482, DOI: 10.1186/1471-2350-7-68.Peer-Reviewed Original ResearchConceptsOtitis mediaWhite infantsInfant respiratory symptomsPhysician-diagnosed asthmaCohort of infantsLarger confirmatory studiesSurfactant proteinsInnate immune responseMonths of ageQuarterly telephone interviewsLogistic regression modelsRace/ethnicityRespiratory symptomsCommon infectionsProspective studyImmune responseStudy populationInfant's birthStandardized questionnaireInfantsConclusionThese resultsAsthmaTelephone interviewsConfirmatory studiesWhole blood
2005
Genomic Subtraction Followed by Dot Blot Screening of Streptococcus pneumoniae Clinical and Carriage Isolates Identifies Genetic Differences Associated with Strains That Cause Otitis Media
Pettigrew MM, Fennie KP. Genomic Subtraction Followed by Dot Blot Screening of Streptococcus pneumoniae Clinical and Carriage Isolates Identifies Genetic Differences Associated with Strains That Cause Otitis Media. Infection And Immunity 2005, 73: 2805-2811. PMID: 15845484, PMCID: PMC1087362, DOI: 10.1128/iai.73.5.2805-2811.2005.Peer-Reviewed Original ResearchConceptsMiddle ear isolatesEar isolatesOtitis mediaMiddle earOtitis media pathogenesisCerebrospinal fluid isolatesBacterial otitis mediaStreptococcus pneumoniae strainsMolecular epidemiological approachSpecific bacterial factorsCarriage isolatesPneumoniae strainsBacterial factorsDot-blot screeningBloodBrucella melitensisEpidemiological approachMeningitisFurther studiesYoung childrenPrecise roleCarriageMedium virulenceIsolatesEar
2004
Association of early‐onset otitis media in infants and exposure to household mould
Pettigrew MM, Gent JF, Triche EW, Belanger KD, Bracken MB, Leaderer BP. Association of early‐onset otitis media in infants and exposure to household mould. Paediatric And Perinatal Epidemiology 2004, 18: 441-447. PMID: 15535820, DOI: 10.1111/j.1365-3016.2004.00596.x.Peer-Reviewed Original ResearchConceptsEarly otitis mediaOtitis mediaMonths of ageHousehold moldInfant respiratory symptomsAcute otitis mediaPhysician-diagnosed asthmaCohort of infantsOtitis media episodesRecurrent otitis mediaEarly onset otitis mediaMonths of lifeQuarterly telephone interviewsRespiratory symptomsCommon infectionsProspective studyPotential confoundersHigh riskInfant's birthStandardised questionnaireTelephone interviewsMonthsAsthmaEarly childhoodFurther studiesDiversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers
FARJO RS, FOXMAN B, PATEL MJ, ZHANG L, PETTIGREW MM, MCCOY SI, MARRS CF, GILSDORF JR. Diversity and sharing of Haemophilus influenzae strains colonizing healthy children attending day-care centers. The Pediatric Infectious Disease Journal 2004, 23: 41-46. PMID: 14743045, DOI: 10.1097/01.inf.0000106981.89572.d1.Peer-Reviewed Original ResearchMeSH KeywordsCarrier StateChild Day Care CentersChild, PreschoolCohort StudiesColony Count, MicrobialDNA, BacterialElectrophoresis, Gel, Pulsed-FieldFemaleGenotypeHaemophilus InfectionsHaemophilus influenzaeHumansIncidenceMaleMass ScreeningMichiganOtitis MediaPolymerase Chain ReactionReference ValuesRisk AssessmentSerotypingConceptsDay care centersH. influenzaeHealthy childrenH. influenzae organismsAcute otitis mediaH. influenzae colonizationH. influenzae strainsUnique strainsEnterobacterial repetitive intergenic consensus PCRThroat culturesOtitis mediaInitial screening techniqueNasopharyngeal colonizationPrevalence ratesColonizing strainsHaemophilus influenzaeHeterogeneity of strainInfluenzaeConsensus PCRRate of colonizationChildrenField gel electrophoresisSame strainPathogenic microorganismsEvidence of sharingInfant Otitis Media and the Use of Secondary Heating Sources
Pettigrew MM, Gent JF, Triche EW, Belanger KD, Bracken MB, Leaderer BP. Infant Otitis Media and the Use of Secondary Heating Sources. Epidemiology 2004, 15: 13-20. PMID: 14712142, DOI: 10.1097/01.ede.0000101292.41006.2e.Peer-Reviewed Original ResearchConceptsRecurrent otitis mediaOtitis media episodesOtitis mediaOtitis media riskOffice visitsDay careMaternal historyWhite raceAssociation of exposureHome heating sourcesDoctor office visitsLogistic regression modelingMedium riskProspective studyPotential confoundersMore episodesSecondary heating sourcesVirginia HospitalWood stove useLogistic regressionTelephone interviewsMultivariate modelAdditional childrenIntermittent useCare
2003
Molecular epidemiology of Streptococcus pneumoniae mediated otitis media.
McCoy S, Pettigrew M. Molecular epidemiology of Streptococcus pneumoniae mediated otitis media. Frontiers In Bioscience-Landmark 2003, 8: e87-93. PMID: 12456346, DOI: 10.2741/961.Peer-Reviewed Original ResearchMeSH KeywordsHumansMolecular EpidemiologyOtitis MediaPneumococcal InfectionsStreptococcus pneumoniae