2007
Roles for Drosophila melanogaster Myosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial Pathogen Pseudomonas entomophila
Hegan PS, Mermall V, Tilney LG, Mooseker MS. Roles for Drosophila melanogaster Myosin IB in Maintenance of Enterocyte Brush-Border Structure and Resistance to the Bacterial Pathogen Pseudomonas entomophila. Molecular Biology Of The Cell 2007, 18: 4625-4636. PMID: 17855510, PMCID: PMC2043548, DOI: 10.1091/mbc.e07-02-0191.Peer-Reviewed Original ResearchConceptsMutant larvaePseudomonas entomophilaTail domainMyosin IBMidgut epithelial cellsActin filament coreDrosophila genomeApical brush borderMidgut enterocytesBrush borderCytoskeletal defectsLarval gutResponse pathwaysApical localizationMV membraneBrush border structureDeletion mutationsFood uptakeGram-negative bacterial infectionsInnate immune responseMyo1bGut physiologyEntomophilaEpithelial cellsMidgut
2005
Myosin-1a Is Critical for Normal Brush Border Structure and Composition
Tyska M, Mackey A, Huang J, Copeland N, Jenkins N, Mooseker M. Myosin-1a Is Critical for Normal Brush Border Structure and Composition. Molecular Biology Of The Cell 2005, 16: 2443-2457. PMID: 15758024, PMCID: PMC1087248, DOI: 10.1091/mbc.e04-12-1116.Peer-Reviewed Original ResearchConceptsMyosin-1aWhole animal phenotypesWhole animal levelIntermediate filament proteinsEctopic recruitmentFunctional redundancyAnimal phenotypesBrush borderMyosin 1cOvert phenotypeBrush border structureFilament proteinsMembrane componentsCellular levelVertebrate myosinsPhenotypeSigns of stressAnimal levelKnockout miceSignificant perturbationsEnterocytesMultifunctional componentsGenesDistinct changesProtein
2004
A role for myosin-1A in the localization of a brush border disaccharidase
Tyska M, Mooseker M. A role for myosin-1A in the localization of a brush border disaccharidase. Journal Of Cell Biology 2004, 165: 395-405. PMID: 15138292, PMCID: PMC2172191, DOI: 10.1083/jcb.200310031.Peer-Reviewed Original Research
2002
MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells
Tyska M, Mooseker M. MYO1A (Brush Border Myosin I) Dynamics in the Brush Border of LLC-PK1-CL4 Cells. Biophysical Journal 2002, 82: 1869-1883. PMID: 11916846, PMCID: PMC1301984, DOI: 10.1016/s0006-3495(02)75537-9.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsBiophysical PhenomenaBiophysicsCloning, MolecularCytoskeletonElectrophoresis, Polyacrylamide GelGreen Fluorescent ProteinsHumansKidneyKineticsLLC-PK1 CellsLuminescent ProteinsMicroscopy, ConfocalMicroscopy, FluorescenceMicrovilliMyosin Type IPrecipitin TestsProtein Structure, TertiaryRecombinant Fusion ProteinsSubcellular FractionsSwineTime FactorsTransfectionConceptsTail domainLLC-PK1Actin core bundleKidney epithelial cell lineApical targetingActin dynamicsBrush borderActin turnoverGFP-actinBB populationsEpithelial cell lineActin domainsFluorescence recoveryCl4 cellsRapid turnoverApical surfaceMotor domainCore bundleATP depletionCell linesActinTurnoverCellsDomain
1995
Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton
Morgan N, Heintzelman M, Mooseker M. Characterization of Myosin-IA and Myosin-IB, Two Unconventional Myosins Associated with theDrosophilaBrush Border Cytoskeleton. Developmental Biology 1995, 172: 51-71. PMID: 7589814, DOI: 10.1006/dbio.1995.0005.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell DifferentiationCloning, MolecularCytoskeletonDrosophila melanogasterElectrophoresis, Polyacrylamide GelEmbryo, NonmammalianFemaleGene Expression RegulationImmunoblottingImmunohistochemistryLarvaMicroscopy, ElectronMicroscopy, ImmunoelectronMicrovilliMyosinsOogenesisOvumPupaRecombinant ProteinsConceptsMyosin IBAdult gutATP-dependent extractionMyosin IASomatic follicle cellsExogenous F-actinBrush border cytoskeletonNovel unconventional myosinDrosophila melanogasterMicrovillus assemblyEgg chambersApical domainMicrovillar componentsMicrovillar domainApical microvillar domainBasolateral domainExpression patternsUnconventional myosinF-actinSubcellular distributionFollicle cellsFunctional myosinDifferentiated enterocytesBiochemical propertiesBrush border
1992
Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2
Peterson M, Mooseker M. Characterization of the enterocyte-like brush border cytoskeleton of the C2BBe clones of the human intestinal cell line, Caco-2. Journal Of Cell Science 1992, 102: 581-600. PMID: 1506435, DOI: 10.1242/jcs.102.3.581.Peer-Reviewed Original ResearchConceptsMyosin IBB myosin IC2BBe cellsATP-dependent associationBrush border cytoskeletonApical brush borderHeavy chainDomain-specific monoclonal antibodiesBrush borderFilter-grown monolayersCaco-2Cell line Caco-2Human colonic epithelial cellsHuman intestinal cell lineMyosin I.Myosin IIIntestinal cell linePhysical associationLaser scanning confocal microscopyProtein villinCytoskeletonCell culture modelCell typesPattern of distributionVivo structureThe molecular architecture of an insect midgut brush border cytoskeleton.
Bonfanti P, Colombo A, Heintzelman M, Mooseker M, Camatini M. The molecular architecture of an insect midgut brush border cytoskeleton. European Journal Of Cell Biology 1992, 57: 298-307. PMID: 1511705.Peer-Reviewed Original ResearchConceptsTwo-dimensional gel analysisMolecular architectureMultiple isoelectric variantsBrush border cytoskeletonLepidopteran larvaeBB myosin IVertebrate intestineBrush borderMyosin IOrganelle exclusionHeavy meromyosin decorationCytoskeletal apparatusMyosin IINonmuscle cellsActin filamentsCytoskeletonIsoelectric variantsGel analysisInstar larvaeMajor isoformsStructural organizationRhodamine phalloidinCore proteinIntestinal brush borderMidgut
1988
Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms
West A, Isaac C, Carboni J, Morrow J, Mooseker M, Barwick K. Localization of villin, a cytoskeletal protein specific to microvilli, in human ileum and colon and in colonic neoplasms. Gastroenterology 1988, 94: 343-352. PMID: 3335311, DOI: 10.1016/0016-5085(88)90421-0.Peer-Reviewed Original Research
1987
Characterization of intestinal brush border cytoskeletal proteins of normal and neoplastic human epithelial cells. A comparison with the avian brush border.
Carboni J, Howe C, West A, Barwick K, Mooseker M, Morrow J. Characterization of intestinal brush border cytoskeletal proteins of normal and neoplastic human epithelial cells. A comparison with the avian brush border. American Journal Of Pathology 1987, 129: 589-600. PMID: 3425692, PMCID: PMC1899811.Peer-Reviewed Original ResearchConceptsMicrovillar actin bundlesActin binding proteinsHuman brush borderIntestinal epithelial cell brush bordersEpithelial cell brush bordersBrush borderMicrovillar proteinsHuman epithelial cellsCytoskeletal matrixCytoskeletal proteinsMultiple proteinsActin bundlesImmunolocalization studiesSpectrin isoformsMammalian sourcesMajor proteinsDifferentiation stateBinding proteinProtein myosinProteinTerminal webCell brush borderCytoskeletonNeoplastic stateMature enterocytes
1986
Cytoskeletal proteins of the rat kidney proximal tubule brush border.
Rodman J, Mooseker M, Farquhar M. Cytoskeletal proteins of the rat kidney proximal tubule brush border. European Journal Of Cell Biology 1986, 42: 319-27. PMID: 3545840.Peer-Reviewed Original ResearchConceptsProximal tubule cellsBrush borderIntestinal brush borderTubule cellsProximal tubule brush borderKidney proximal tubule cellsTubule brush borderRat kidney proximal tubule cellsTerminal web regionKidney brush borderTerminal webImmunogold labeling procedureKidneyCell typesImmunoelectron microscopyBasolateral membraneCytoskeletal componentsVillinCytoskeletal proteinsMicrovilli
1985
Role of myosin in terminal web contraction in isolated intestinal epithelial brush borders.
Keller T, Conzelman K, Chasan R, Mooseker M. Role of myosin in terminal web contraction in isolated intestinal epithelial brush borders. Journal Of Cell Biology 1985, 100: 1647-1655. PMID: 3988804, PMCID: PMC2113869, DOI: 10.1083/jcb.100.5.1647.Peer-Reviewed Original Research
1984
Calcium-dependent regulation of cytoskeletal structure and contractility in the intestinal brush border.
Mooseker M. Calcium-dependent regulation of cytoskeletal structure and contractility in the intestinal brush border. Progress In Clinical And Biological Research 1984, 168: 179-86. PMID: 6542665.Peer-Reviewed Original Research
1983
Regulation of Cytoskeletal Structure and Contractility in the Brush Border
Mooseker M, Keller T, Hirokawa N. Regulation of Cytoskeletal Structure and Contractility in the Brush Border. Novartis Foundation Symposia 1983, 95: 195-215. PMID: 6552204, DOI: 10.1002/9780470720769.ch12.Peer-Reviewed Original ResearchConceptsIntestinal epithelial cellsBrush borderEpithelial cellsRat brush bordersIsometric contractionFree Ca2ContractilityBrush border proteinsRegulation of forceContractionChicken intestineAnalysis of Ca2Light chainCa2Microvillar rootletsActin-severing proteinBrush border myosinPresence of Ca2Circumferential bundlesCalmodulin functionTerminal web regionCalciumCellsPhosphorylationCytoskeletal structures
1982
Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro.
Keller T, Mooseker M. Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitro. Journal Of Cell Biology 1982, 95: 943-959. PMID: 6897550, PMCID: PMC2112925, DOI: 10.1083/jcb.95.3.943.Peer-Reviewed Original ResearchConceptsBrush border contractionBrush borderIntestinal epithelial cellsEpithelial cellsCalmodulin activityBrush border proteinsMyosin light chain kinaseContractionDegrees CLight chain kinaseLight chainCalmodulin-dependent phosphorylationBrush border myosinPhosphorylationDalton light chainChain kinaseTerminal webChapter 8 The Brush Border of Intestinal Epithelium: A Model System for Analysis of Cell-Surface Architecture and Motility
Mooseker M, Howe C. Chapter 8 The Brush Border of Intestinal Epithelium: A Model System for Analysis of Cell-Surface Architecture and Motility. Methods In Cell Biology 1982, 25: 143-174. PMID: 7109959, DOI: 10.1016/s0091-679x(08)61424-7.Chapters