2014
Methylation of Twelve CpGs in Human Papillomavirus Type 16 (HPV16) as an Informative Biomarker for the Triage of Women Positive for HPV16 Infection
Brandsma JL, Harigopal M, Kiviat NB, Sun Y, Deng Y, Zelterman D, Lizardi PM, Shabanova VS, Levi A, Yaping T, Hu X, Feng Q. Methylation of Twelve CpGs in Human Papillomavirus Type 16 (HPV16) as an Informative Biomarker for the Triage of Women Positive for HPV16 Infection. Cancer Prevention Research 2014, 7: 526-533. PMID: 24556390, DOI: 10.1158/1940-6207.capr-13-0354.Peer-Reviewed Original ResearchConceptsCervical intraepithelial neoplasiaCytology samplesCutoff scoreColposcopy of womenCervical cancer preventionManagement of womenDetection of CIN3Cervical cytology samplesHuman papillomavirus type 16Human papillomavirus type 16 DNAMethylation biomarkersPapillomavirus type 16Histologic severityIntraepithelial neoplasiaCervical cancerHPV16 infectionCancer preventionDNA bisulfite sequencingType 16Prognostic potentialAccurate biomarkersProspective biomarkersScreening testInformative biomarkersBiomarkers
2011
Use of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions
Levi AW, Harigopal M, Hui P, Schofield K, Chhieng DC. Use of high‐risk human papillomavirus testing in patients with low‐grade squamous intraepithelial lesions. Cancer Cytopathology 2011, 119: 228-234. PMID: 21732551, DOI: 10.1002/cncy.20172.Peer-Reviewed Original ResearchConceptsLow-grade squamous intraepithelial lesionsHR-HPV testingHPV testingCIN 2Colposcopy rateHR-HPVRelative light units/cutoffCervical intraepithelial neoplasia 2High-risk human papillomavirusHR-HPV statusRLU/COIntraepithelial neoplasia 2Hybrid Capture 2Squamous intraepithelial lesionsCIN 1 lesionsLarge teaching hospitalNegative predictive valueLSIL cytologyHPV statusIntraepithelial lesionsCapture 2Human papillomavirusTeaching hospitalCytologic diagnosisSevere lesions
2009
Distinct human papillomavirus type 16 methylomes in cervical cells at different stages of premalignancy
Brandsma JL, Sun Y, Lizardi PM, Tuck DP, Zelterman D, Haines GK, Martel M, Harigopal M, Schofield K, Neapolitano M. Distinct human papillomavirus type 16 methylomes in cervical cells at different stages of premalignancy. Virology 2009, 389: 100-107. PMID: 19443004, PMCID: PMC2918277, DOI: 10.1016/j.virol.2009.03.029.Peer-Reviewed Original ResearchConceptsRoutine cervical cancer screeningCervical cellsCervical cancer screeningHigh-grade lesionsLow-grade lesionsHPV16-positive samplesCervical progressionNegative cytologyCervical lesionsCancer screeningCervical samplesPathologic diagnosisCervical carcinogenesisClinical significanceHuman papillomavirus gene expressionLow-level methylationLarger studyE6 geneL1 regionLesionsAbnormal patternsClinical samplesPattern ADistinct methylation patternsHigh-level methylation
2006
Molecular Classification Identifies a Subset of Human Papillomavirus–Associated Oropharyngeal Cancers With Favorable Prognosis
Weinberger PM, Yu Z, Haffty BG, Kowalski D, Harigopal M, Brandsma J, Sasaki C, Joe J, Camp RL, Rimm DL, Psyrri A. Molecular Classification Identifies a Subset of Human Papillomavirus–Associated Oropharyngeal Cancers With Favorable Prognosis. Journal Of Clinical Oncology 2006, 24: 736-747. PMID: 16401683, DOI: 10.1200/jco.2004.00.3335.Peer-Reviewed Original ResearchConceptsOropharyngeal squamous cell carcinomaHuman papillomavirusFavorable prognosisClass IIILocal recurrencePrognostic valueHuman Papillomavirus–Associated Oropharyngeal CancerHPV DNA presenceHPV16 viral loadDisease-free survivalMultivariable survival analysisSquamous cell carcinomaLong-term patientsThree-class modelReal-time polymerase chain reactionHPV statusLow p53Only patientsOverall survivalOropharyngeal cancerViral loadCell carcinomaPolymerase chain reactionClinical trialsP16 overexpression
2004
Comparison of human papillomavirus DNA prevalence in atypical squamous cells of undetermined significance subcategories as defined by the original Bethesda 1991 and the new Bethesda 2001 Systems.
Pirog EC, Erroll M, Harigopal M, Centeno BA. Comparison of human papillomavirus DNA prevalence in atypical squamous cells of undetermined significance subcategories as defined by the original Bethesda 1991 and the new Bethesda 2001 Systems. Archives Of Pathology & Laboratory Medicine 2004, 128: 527-32. PMID: 15086283, DOI: 10.5858/2004-128-527-cohpdp.Peer-Reviewed Original ResearchConceptsAtypical squamous cellsHigh-grade squamous intraepithelial lesionsTBS 2001Squamous intraepithelial lesionsSquamous cellsASCUS casesIntraepithelial lesionsIndividual morphologic featuresMorphologic featuresHPV DNAHPV detectionPap testAtypical cellsUndetermined significanceBroad-spectrum HPV DNA amplificationControl groupHuman papillomavirus (HPV) DNA prevalenceASC-H casesBethesda 2001 systemPrevalence of HPVThinPrep Pap testsHPV DNA amplificationHuman papillomavirus DNAPanel of pathologistsBethesda System 2001