2005
Gene Therapy for Autosomal Dominant Disorders of Keratin
Lewin AS, Glazer PM, Milstone LM. Gene Therapy for Autosomal Dominant Disorders of Keratin. Journal Of Investigative Dermatology Symposium Proceedings 2005, 10: 47-61. PMID: 16250209, DOI: 10.1111/j.1087-0024.2005.10207.x.Commentaries, Editorials and LettersMeSH KeywordsAnimalsDarier DiseaseDependovirusEctodermal DysplasiaEpidermolysis Bullosa SimplexGene SilencingGene TargetingGenes, DominantGenetic TherapyGenetic VectorsHumansKeratinsKeratoderma, PalmoplantarMiceMutationNails, MalformedOligonucleotides, AntisenseRNA InterferenceRNA, CatalyticRNA, Small InterferingConceptsRNA knockdown approachGene correctionGene therapyViral vectorsEpidermal skin cellsKeratin diseasesKnockdown approachRNA interferenceGene expressionEpidermolysis bullosa simplexToxic proteinsDominant mutationsGenetic diseasesGenetic therapiesKeratin filamentsEpidermal diseasesGenetic defectsTissue cultureSelective inhibitorSkin cellsAttractive alternativeAutosomal dominant disorderRecent innovationsDominant disorderNear future
1976
δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells
Milstone L, Zelenka P, Piatigorsky J. δ-Crystallin mRNA in chick lens cells: mRNA accumulates during differential stimulation of δ-crystallin synthesis in cultured cells. Developmental Biology 1976, 48: 197-204. PMID: 1254080, DOI: 10.1016/0012-1606(76)90084-1.Peer-Reviewed Original ResearchConceptsΔ-crystallin synthesisChick lens epithelial cellsChick lens cellsEmbryonic lens epitheliumLens fiber cellsLens epithelial cellsFiber cellsLens cellsDifferential stimulationProtein synthesisCultured cellsMRNALens epitheliumEpithelial cellsTissue cultureCellsDifferentiationOverall stimulationAccumulationStimulationSpecializationCultureSynthesisOvo
1973
Differentiation of lens fibers in explanted embryonic chick lens epithelia
Piatigorsky J, Rothschild S, Milstone L. Differentiation of lens fibers in explanted embryonic chick lens epithelia. Developmental Biology 1973, 34: 334-345. PMID: 4596973, DOI: 10.1016/0012-1606(73)90362-x.Peer-Reviewed Original ResearchConceptsChick lens epitheliumLens epitheliumLens fibersLens epithelial cellsRibosomal clustersRibosomal RNADelta-crystallinTissue cultureSingle-strand breaksFiber differentiationCell membraneLong cellsMean cell lengthCell lengthTotal RNACellular outgrowthDifferentiationEpithelial cellsSurface membraneRNAOrganelle densityChick embryosGranular matrixBiochemical changesCells