2021
Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles
Wu XS, Subramanian S, Zhang Y, Shi B, Xia J, Li T, Guo X, El-Hassar L, Szigeti-Buck K, Henao-Mejia J, Flavell RA, Horvath TL, Jonas EA, Kaczmarek LK, Wu LG. Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles. Neuron 2021, 109: 938-946.e5. PMID: 33508244, PMCID: PMC7979485, DOI: 10.1016/j.neuron.2021.01.006.Peer-Reviewed Original ResearchConceptsSlow endocytosisVesicle mobilizationF-actin cytoskeletonChannel mutationsPotassium channelsKv3.3 proteinsInhibits endocytosisRapid endocytosisNovel functionF-actinEndocytosisCrucial functionSynaptic vesiclesFamily channelsSynaptic transmissionDiscovery decadesMembrane potentialNeurotransmitter releaseDiverse neurological disordersIon conductanceMutationsReleasable poolMouse nerve terminalsPotassium channel mutationsPathological effects
2015
How Neurons Communicate: Gap Junctions and Neurosecretion
Levitan I, Kaczmarek L. How Neurons Communicate: Gap Junctions and Neurosecretion. 2015, 153-186. DOI: 10.1093/med/9780199773893.003.0008.ChaptersSynaptic vesiclesSNARE complex proteinsExistence of proteinsSecretion of neurotransmittersComplex proteinsPlasma membraneCytoplasmic vesiclesProteinVesiclesAdjacent cellsGap junctionsSmall moleculesCalcium entryExternal mediumElectrical couplingMembraneNeurosecretionCellsComplex processEndocytosisExocytosisFluorescent dyeMoleculesConnexinsCytoplasmSynaptic Release of Neurotransmitters
Levitan I, Kaczmarek L. Synaptic Release of Neurotransmitters. 2015, 187-212. DOI: 10.1093/med/9780199773893.003.0009.ChaptersSynaptic vesiclesIndividual synaptic vesiclesRapid synaptic transmissionGenetic experimentsVoltage-dependent channelsEntry of calciumAmount of transmitterRelease of neurotransmittersSquid stellate ganglionFrog neuromuscular junctionNeurotransmitter releasePhysiological experimentsStellate ganglionSpecific synapsesSpecialized synapsesSynaptic transmissionSynaptic releaseRepetitive stimulationVesiclesPresynaptic terminalsNeuromuscular junctionAction potentialsFurther insightNeurotransmittersExocytosis
1998
High-frequency firing helps replenish the readily releasable pool of synaptic vesicles
Wang L, Kaczmarek L. High-frequency firing helps replenish the readily releasable pool of synaptic vesicles. Nature 1998, 394: 384-388. PMID: 9690475, DOI: 10.1038/28645.Peer-Reviewed Original ResearchConceptsReleasable poolPotassium channel blocker tetraethylammoniumChannel blocker Cd2Synaptic vesiclesPresynaptic action potentialHigh-frequency stimulationVoltage-gated Ca2Short-term synaptic depressionCentral nervous systemPatch-clamp recordingsHigh-frequency firingGiant synapsesPostsynaptic mechanismsBuffer EGTAMouse auditoryBlocker tetraethylammoniumSynaptic activitySynaptic depressionPresynaptic terminalsNervous systemAction potentialsRate of replenishmentSynapsesCa2Key signal