Megakaryoblastic Leukemia Factor-1 Transduces Cytoskeletal Signals and Induces Smooth Muscle Cell Differentiation from Undifferentiated Embryonic Stem Cells*
Du KL, Chen M, Li J, Lepore JJ, Mericko P, Parmacek MS. Megakaryoblastic Leukemia Factor-1 Transduces Cytoskeletal Signals and Induces Smooth Muscle Cell Differentiation from Undifferentiated Embryonic Stem Cells*. Journal Of Biological Chemistry 2004, 279: 17578-17586. PMID: 14970199, DOI: 10.1074/jbc.m400961200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, NorthernCell DifferentiationCell NucleusCells, CulturedChromatinCOS CellsCytoplasmCytoskeletonDNA-Binding ProteinsDNA, ComplementaryEmbryo, MammalianGenes, DominantHumansImmunohistochemistryLuciferasesMiceMyocytes, Smooth MuscleNIH 3T3 CellsNuclear ProteinsOncogene Proteins, FusionPlasmidsPrecipitin TestsPromoter Regions, GeneticProtein BindingProtein Structure, TertiaryProtein TransportReverse Transcriptase Polymerase Chain ReactionSignal TransductionStem CellsTissue DistributionTrans-ActivatorsTranscriptional ActivationTransfectionTwo-Hybrid System TechniquesConceptsSmooth muscle cell differentiationMuscle cell differentiationEmbryonic stem cellsPromoter/enhancerSmooth muscle cellsRhoA signalingSM22alpha promoterCell differentiationUndifferentiated embryonic stem cellsSerum response factorTranscription factor myocardinLeucine zipper domainTranscriptional regulatory elementsStem cellsMost human tissuesCytoskeletal signalsTranscriptional programsTransduce signalsMKL1 mutantSMC differentiationZipper domainRegulatory elementsActin polymerizationMKL1 geneSerum stimulation