2023
Sorting secretory proteins
Parchure A, von Blume J. Sorting secretory proteins. ELife 2023, 12: e93490. PMID: 37997893, PMCID: PMC10672786, DOI: 10.7554/elife.93490.Peer-Reviewed Original Research
2021
Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells.
Pakdel M, Pacheco-Fernandez N, von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Live-cell Vesicle Tracking in the Secretory Pathway Using HeLa Cells. Bio-protocol 2021, 11: e3958. PMID: 33855118, PMCID: PMC8032492, DOI: 10.21769/bioprotoc.3958.Peer-Reviewed Original ResearchProtein traffickingVesicle traffickingSecretory pathwayGolgi vesicle traffickingSelective hooks (RUSH) systemHeLa cellsLive-cell microscopyMammalian cellsProtein cargoLive-cell monitoringCargo vesiclesVesicle trackingTraffickingRUSH systemSorting assaysWide-field microscopyPhysiological conditionsVesiclesMore detail processesPathwayCargoCellsFinal destinationProteinSecretorySending out molecules from the TGN
Ramazanov BR, Tran ML, von Blume J. Sending out molecules from the TGN. Current Opinion In Cell Biology 2021, 71: 55-62. PMID: 33706234, PMCID: PMC8328904, DOI: 10.1016/j.ceb.2021.02.005.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkEndoplasmic reticulum contact sitesCell-cell communicationExtracellular matrix proteinsMembrane traffickingCargo proteinsCargo sortingTGN structureTransmembrane proteinPlasma membraneContact sitesExciting linkSoluble proteinMatrix proteinsSorting mechanismProteinTransport carriersMajor open questionsSortingMembraneNovel typeTraffickingMachineryOrganismsVesiclesRetention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells.
Pacheco-Fernandez N, Pakdel M, Von Blume J. Retention Using Selective Hooks (RUSH) Cargo Sorting Assay for Protein Vesicle Tracking in HeLa Cells. Bio-protocol 2021, 11: e3936. PMID: 33796610, PMCID: PMC8005885, DOI: 10.21769/bioprotoc.3936.Peer-Reviewed Original ResearchSecretory pathwaySelective hooks (RUSH) systemProtein sortingVesicle traffickingSpecific cargoCargo traffickingProtein cargoTrafficking dynamicsProtein dynamicsVesicle trackingBiotin additionHeLa cellsFluorescent vesiclesTraffickingSorting assaysPathwayCargoCellsProteinExcellent toolSuch studiesVesiclesBetter understandingRegulationSorting
2020
Fam20C regulates protein secretion by Cab45 phosphorylation
Hecht TK, Blank B, Steger M, Lopez V, Beck G, Ramazanov B, Mann M, Tagliabracci V, von Blume J. Fam20C regulates protein secretion by Cab45 phosphorylation. Journal Of Cell Biology 2020, 219: e201910089. PMID: 32422653, PMCID: PMC7265331, DOI: 10.1083/jcb.201910089.Peer-Reviewed Original Research