2024
Calcium flow at ER-TGN contact sites facilitates secretory cargo export
Ramazanov B, Parchure A, Di Martino R, Kumar A, Chung M, Kim Y, Griesbeck O, Schwartz M, Luini A, von Blume J. Calcium flow at ER-TGN contact sites facilitates secretory cargo export. Molecular Biology Of The Cell 2024, 35: ar50. PMID: 38294859, PMCID: PMC11064664, DOI: 10.1091/mbc.e23-03-0099.Peer-Reviewed Original Research
2023
Sorting secretory proteins
Parchure A, von Blume J. Sorting secretory proteins. ELife 2023, 12: e93490. PMID: 37997893, PMCID: PMC10672786, DOI: 10.7554/elife.93490.Peer-Reviewed Original Research
2018
Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network
Deng Y, Pakdel M, Blank B, Sundberg EL, Burd CG, von Blume J. Activity of the SPCA1 Calcium Pump Couples Sphingomyelin Synthesis to Sorting of Secretory Proteins in the Trans-Golgi Network. Developmental Cell 2018, 47: 464-478.e8. PMID: 30393074, PMCID: PMC6261503, DOI: 10.1016/j.devcel.2018.10.012.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkTGN membranesCell surface transportProtein sortingTrafficking pathwaysSecretion pathwaySecretory proteinsSphingomyelin synthesisSorting mechanismLipid synthesisProteinVesicular carriersCab45Sphingomyelin contentNew lipidSortingPrincipal functionMembranePathwayCore componentLipidsGolgiSurface transportExportActivityExploring new routes for secretory protein export from the trans-Golgi network
Pakdel M, von Blume J. Exploring new routes for secretory protein export from the trans-Golgi network. Molecular Biology Of The Cell 2018, 29: 235-240. PMID: 29382805, PMCID: PMC5996961, DOI: 10.1091/mbc.e17-02-0117.Peer-Reviewed Original ResearchConceptsSecretory cargoSecretory proteinsCytoplasmic actin cytoskeletonMajor sorting stationTrans-Golgi networkGolgi residentsProtein exportGolgi networkActin cytoskeletonATPase SPCA1Cellular compartmentsTissue homeostasisSorting stationSoluble proteinTGNLysosomal hydrolasesOverall pathwayLuminal CaRecent discoveryProteinCargoSpecific carrierSortingCellsFinal destination
2017
Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network
Blank B, von Blume J. Cab45—Unraveling key features of a novel secretory cargo sorter at the trans-Golgi network. European Journal Of Cell Biology 2017, 96: 383-390. PMID: 28372832, DOI: 10.1016/j.ejcb.2017.03.001.Peer-Reviewed Original ResearchConceptsMolecular functionsCargo moleculesCytoplasmic actin cytoskeletonSpecific cargo moleculesTransmembrane cargo receptorsTrans-Golgi networkMannose-6-phosphate receptorCargo receptorsGolgi networkActin cytoskeletonATPase SPCA1Secreted proteinsPlasma membraneSecretory proteinsExtracellular proteasesCab45Sorting mechanismSurface receptorsProteinExtracellular spaceSpecific carrierPossible roleFinal destinationSortingReceptors
2016
Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network
Crevenna AH, Blank B, Maiser A, Emin D, Prescher J, Beck G, Kienzle C, Bartnik K, Habermann B, Pakdel M, Leonhardt H, Lamb DC, von Blume J. Secretory cargo sorting by Ca2+-dependent Cab45 oligomerization at the trans-Golgi network. Journal Of Cell Biology 2016, 213: 305-314. PMID: 27138253, PMCID: PMC4862333, DOI: 10.1083/jcb.201601089.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkSecretory cargoSecretory proteinsSpecific cargo moleculesTransmembrane cargoCargo moleculesSuperresolution microscopyIntact cellsCab45Lysosomal hydrolasesExtracellular spaceDependent mannerCargoProteinOligomerizationSortingHydrolasesDependent changesMicrodomainsMutationsOligomersElucidationCellsCa2
2014
Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting
Kienzle C, Basnet N, Crevenna AH, Beck G, Habermann B, Mizuno N, von Blume J. Cofilin recruits F-actin to SPCA1 and promotes Ca2+-mediated secretory cargo sorting. Journal Of Cell Biology 2014, 206: 635-654. PMID: 25179631, PMCID: PMC4151145, DOI: 10.1083/jcb.201311052.Peer-Reviewed Original Research
2012
Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast
Curwin AJ, von Blume J, Malhotra V. Cofilin-mediated sorting and export of specific cargo from the Golgi apparatus in yeast. Molecular Biology Of The Cell 2012, 23: 2327-2338. PMID: 22553351, PMCID: PMC3374751, DOI: 10.1091/mbc.e11-09-0826.Peer-Reviewed Original ResearchMeSH KeywordsActin Depolymerizing FactorsBlotting, WesternCathepsin ACell MembraneCofilin 1EndocytosisGlucan Endo-1,3-beta-D-GlucosidaseGreen Fluorescent ProteinsIntracellular MembranesMicroscopy, FluorescenceMutationProtein TransportProton-Translocating ATPasesR-SNARE ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTrans-Golgi NetworkVacuolesConceptsTrans-Golgi networkLate Golgi membranesCarboxypeptidase YSecretory cargoGolgi membranesLate Golgi compartmentMechanisms of cargoSoluble secretory proteinsRole of cofilinATPase Pma1Yeast orthologueSynthetic sicknessProtein cofilinSpecific cargoMutant cellsMammalian cellsGolgi compartmentSorting defectsPlasma membraneSecretory proteinsGolgi apparatusATPase 1CofilinCell surfacePMR1
2009
Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network
von Blume J, Duran JM, Forlanelli E, Alleaume AM, Egorov M, Polishchuk R, Molina H, Malhotra V. Actin remodeling by ADF/cofilin is required for cargo sorting at the trans-Golgi network. Journal Of Cell Biology 2009, 187: 1055-1069. PMID: 20026655, PMCID: PMC2806282, DOI: 10.1083/jcb.200908040.Peer-Reviewed Original ResearchConceptsTrans-Golgi networkADF/cofilinActin-depolymerizing factorSecretory proteinsSecretory cargoCofilin knockdownMass spectrometry-based protein profilingMammalian tissue culture cellsGolgi-resident proteinsSorting of proteinsEndogenous secretory proteinsIntegral membrane proteinsSoluble secretory proteinsStable isotope labelingTissue culture cellsDrosophila melanogasterActin remodelingMammalian cellsMembrane proteinsGolgi membranesProtein profilingIsotope labelingCofilinCell surfaceAmino acids