2023
piRNA generation is associated with the pioneer round of translation in stem cells
Parambil S, Li D, Zelko M, Poulet A, van Wolfswinkel J. piRNA generation is associated with the pioneer round of translation in stem cells. Nucleic Acids Research 2023, 52: 2590-2608. PMID: 38142432, PMCID: PMC10954484, DOI: 10.1093/nar/gkad1212.Peer-Reviewed Original ResearchSMEDWI-1Pioneer roundStem cellsPlanarian stem cellsStem cell maintenanceStem cell stateNon-coding transcriptsPiRNA pathwayPIWI proteinsTransposon repressionBiogenesis pathwayPiRNA generationGenomic integritySmall RNAsCell maintenanceSuch transcriptsCell statesCytoplasmic poolTranscriptsCell coloniesCell healthPiRNAsDynamic poolTranscriptomeCells
2012
Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans
Kamminga LM, van Wolfswinkel JC, Luteijn MJ, Kaaij LJ, Bagijn MP, Sapetschnig A, Miska EA, Berezikov E, Ketting RF. Differential Impact of the HEN1 Homolog HENN-1 on 21U and 26G RNAs in the Germline of Caenorhabditis elegans. PLOS Genetics 2012, 8: e1002702. PMID: 22829772, PMCID: PMC3400576, DOI: 10.1371/journal.pgen.1002702.Peer-Reviewed Original ResearchConceptsHENN-1G RNAAGO proteinsSRNA moleculesSmall RNAsGene expressionC. elegans piRNAsGermline-expressed genesSequence-specific recruitmentDifferent RNAi pathwaysGlobal gene expression analysisGlobal gene expressionLoss of methylationGene expression analysisEndogenous RNAiRNAi pathwayC. elegansRNA classesRNA displayGene activityRNA interferenceExpression analysisSequence specificityTarget RNARNA
2009
The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation
Claycomb J, Batista P, Pang K, Gu W, Vasale J, van Wolfswinkel J, Chaves D, Shirayama M, Mitani S, Ketting R, Conte D, Mello C. The Argonaute CSR-1 and Its 22G-RNA Cofactors Are Required for Holocentric Chromosome Segregation. Cell 2009, 139: 123-134. PMID: 19804758, PMCID: PMC2766185, DOI: 10.1016/j.cell.2009.09.014.Peer-Reviewed Original ResearchConceptsArgonaute CSR-1Chromosome segregationHolocentric chromosomesCSR-1Protein-coding genesProper chromosome segregationProtein-coding domainsDRH-3EGO-1C. elegansSmall RNAsTarget lociTarget mRNAsSpindle polesDevelopmental timingMetaphase plateDiverse processesChromosomesKinetochoresProtein levelsProper organizationElegansChromatinDicerGenome