2003
Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease
Biedermann BC, Tsakiris DA, Gregor M, Pober JS, Gratwohl A. Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease. Bone Marrow Transplantation 2003, 32: 1077-1084. PMID: 14625579, DOI: 10.1038/sj.bmt.1704258.Peer-Reviewed Original ResearchMeSH KeywordsBiomarkersCase-Control StudiesEndothelium, VascularFas Ligand ProteinGraft vs Host DiseaseHematopoietic Stem Cell TransplantationInterferon-gammaLymphocyte ActivationMembrane GlycoproteinsMolecular Diagnostic TechniquesRNA, MessengerSensitivity and SpecificitySurvivorsT-Lymphocytes, CytotoxicTransplantation, HomologousVon Willebrand FactorConceptsCytotoxic T lymphocytesEndothelial injuryT cell activationT cellsT lymphocytesHost diseaseSCT recipientsAllogeneic stem cell transplantationAllospecific cytotoxic T lymphocytesIFN-γ transcript levelsActive chronic GVHDEndothelial injury resultsCD8 T lymphocytesCD8 T cellsCD4 T cellsLong-term survivorsStem cell transplantationImportant effector cellsVon Willebrand factor plasma levelsAge-matched controlsVascular endothelial cellsChronic GVHDActive graftEffector cellsCell transplantation
1994
Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells
Birch K, Ewenstein B, Golan D, Pober J. Prolonged peak elevations in cytoplasmic free calcium ions, derived from intracellular stores, correlate with the extent of thrombin‐stimulated exocytosis in single human umbilical vein endothelial cells. Journal Of Cellular Physiology 1994, 160: 545-554. PMID: 7521337, DOI: 10.1002/jcp.1041600318.Peer-Reviewed Original ResearchConceptsEndothelial cellsAgonist concentrationsSingle human umbilical vein endothelial cellCytoplasmic free calcium ionsNumber of cellsPeak elevationHigh agonist concentrationsCytoplasmic free calciumHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsVWF secretionIndividual endothelial cellsAmount of secretionVein endothelial cellsHuman endothelial cellsAgonist treatmentVon Willebrand factor secretionAgonist doseExtracellular Ca2Maximal secretionP-selectinThrombin receptorHigh dosesIntracellular storesFactor secretion
1992
Calcium/calmodulin transduces thrombin-stimulated secretion: studies in intact and minimally permeabilized human umbilical vein endothelial cells.
Birch KA, Pober JS, Zavoico GB, Means AR, Ewenstein BM. Calcium/calmodulin transduces thrombin-stimulated secretion: studies in intact and minimally permeabilized human umbilical vein endothelial cells. Journal Of Cell Biology 1992, 118: 1501-1510. PMID: 1522120, PMCID: PMC2289613, DOI: 10.1083/jcb.118.6.1501.Peer-Reviewed Original ResearchConceptsVon Willebrand factorEndothelial cellsProtein kinase CCell-permeant calcium chelatorExtracellular calcium chelator EGTAVWF secretionAgonist-induced elevationInhibitory peptidesSignal transduction pathwaysHuman umbilical vein endothelial cellsUmbilical vein endothelial cellsCultured endothelial cellsCalcium chelator EGTAPermeabilized human umbilical vein endothelial cellsVein endothelial cellsHuman endothelial cellsPhorbol esterRole of calmodulinSecond messenger pathwaysIntracellular calciumIntact human endothelial cellsTransduction pathwaysThrombin receptorCalcium ionophoreLarge intracellular protein
1989
IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis.
Zavoico GB, Ewenstein BM, Schafer AI, Pober JS. IL-1 and related cytokines enhance thrombin-stimulated PGI2 production in cultured endothelial cells without affecting thrombin-stimulated von Willebrand factor secretion or platelet-activating factor biosynthesis. The Journal Of Immunology 1989, 142: 3993-9. PMID: 2497185, DOI: 10.4049/jimmunol.142.11.3993.Peer-Reviewed Original ResearchMeSH KeywordsArachidonic AcidArachidonic AcidsCells, CulturedChromatography, Thin LayerCycloheximideDrug SynergismEndothelium, VascularEpoprostenolHistamineHumansInterleukin-1Platelet Activating FactorProstaglandin Endoperoxides, SyntheticProstaglandin H2Prostaglandins HThrombinVon Willebrand Factor
1987
Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells.
Ewenstein BM, Warhol MJ, Handin RI, Pober JS. Composition of the von Willebrand factor storage organelle (Weibel-Palade body) isolated from cultured human umbilical vein endothelial cells. Journal Of Cell Biology 1987, 104: 1423-1433. PMID: 3494734, PMCID: PMC2114468, DOI: 10.1083/jcb.104.5.1423.Peer-Reviewed Original ResearchMeSH KeywordsCell FractionationCells, CulturedEndotheliumEnzyme-Linked Immunosorbent AssayFemaleHumansMicroscopy, ElectronOrganoidsUmbilical VeinsVon Willebrand FactorConceptsWeibel-Palade bodiesSecretory organellesUmbilical vein endothelial cellsOrganelle fractionsVein endothelial cellsCultured endothelial cellsIntracellular protein traffickingVon Willebrand factorVon Willebrand factor storageEndothelial cellsSelf-generating Percoll gradientsDirect morphological examinationCultured umbilical vein endothelial cellsUnmodified formProtein traffickingCultured human umbilical vein endothelial cellsRegulated secretionStimulation of ECHuman umbilical vein endothelial cellsStorage organellesOrganellesDense organellesPro sequenceProteolytic processingAdhesive glycoprotein