2015
Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation
Wisnewski AV, Liu J, Colangelo CM. Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation. Chemical Research In Toxicology 2015, 28: 729-737. PMID: 25635619, PMCID: PMC4667722, DOI: 10.1021/tx5005002.Peer-Reviewed Original ResearchConceptsEosinophilic inflammatory responseAlternative macrophage activationInflammatory responseChemical allergensMacrophage activationAirway eosinophil numbersEosinophilic airway inflammationInnate immune responseGreater inflammatory responseAirway levelsMDI conjugationAirway inflammationOccupational asthmaIL-12MDI exposureAsthma pathogenesisEosinophil numbersNaïve miceAirway fluidRespiratory tractImmune responseMucus productionSkin exposureMDI conjugatesVivo evidence
2008
Human innate immune responses to hexamethylene diisocyanate (HDI) and HDI–albumin conjugates
Wisnewski AV, Liu Q, Liu J, Redlich CA. Human innate immune responses to hexamethylene diisocyanate (HDI) and HDI–albumin conjugates. Clinical & Experimental Allergy 2008, 38: 957-967. PMID: 18498542, PMCID: PMC3131007, DOI: 10.1111/j.1365-2222.2008.02982.x.Peer-Reviewed Original ResearchMeSH KeywordsAdipokinesAdministration, InhalationAlbuminsAntigens, CDBlotting, WesternCells, CulturedChemokine CCL2Chitinase-3-Like Protein 1ChitinasesCyanatesEnzyme-Linked Immunosorbent AssayGene ExpressionGlycoproteinsHumansImmunity, InnateIntramolecular OxidoreductasesIsocyanatesLectinsLeukocytes, MononuclearLysosomesMacrophage Migration-Inhibitory FactorsMicroscopy, FluorescenceOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionPolymorphism, GeneticProteinsRNAConceptsPeripheral blood mononuclear cellsInnate immune responseImmune responsePercentage of PBMCHuman peripheral blood mononuclear cellsFlow cytometrySystemic innate immune responseExposure-dependent decreaseMonocyte/macrophage traffickingHuman innate immune responseProtein expressionSpecific inhalation challengeBlood mononuclear cellsAdaptive immune responsesLow-density lipoproteinPattern recognition receptorsInhalation challengeOccupational asthmaClinical spectrumControl antigenMononuclear cellsHDI exposureSerum concentrationsAdaptive immunityGene/protein expression