2014
Selection of Drug Resistance-Mediating Plasmodium falciparum Genetic Polymorphisms by Seasonal Malaria Chemoprevention in Burkina Faso
Somé A, Zongo I, Compaoré Y, Sakandé S, Nosten F, Ouédraogo J, Rosenthal P. Selection of Drug Resistance-Mediating Plasmodium falciparum Genetic Polymorphisms by Seasonal Malaria Chemoprevention in Burkina Faso. Antimicrobial Agents And Chemotherapy 2014, 58: 3660-3665. PMID: 24733476, PMCID: PMC4068591, DOI: 10.1128/aac.02406-14.Peer-Reviewed Original ResearchConceptsSeasonal malaria chemopreventionAQ/SPMalaria chemopreventionSingle nucleotide polymorphismsP. falciparum crtPfcrt 76TResistance-mediating polymorphismsMalaria control measuresPCR-positive samplesMonthly DPPfdhfr 108NPfdhfr 51IPfmdr1 86YSulfadoxine-pyrimethamineTreatment armsDevelopment of resistanceTransmission seasonControl groupP. falciparumSNP prevalenceGenetic polymorphismsRegular useAntifolate antimalarialsMonthsBurkina Faso
2013
PlasmoView: A Web-based Resource to Visualise Global Plasmodium falciparum Genomic Variation
Preston M, Assefa S, Ocholla H, Sutherland C, Borrmann S, Nzila A, Michon P, Hien T, Bousema T, Drakeley C, Zongo I, Ouédraogo J, Djimde A, Doumbo O, Nosten F, Fairhurst R, Conway D, Roper C, Clark T. PlasmoView: A Web-based Resource to Visualise Global Plasmodium falciparum Genomic Variation. The Journal Of Infectious Diseases 2013, 209: 1808-1815. PMID: 24338354, PMCID: PMC4017360, DOI: 10.1093/infdis/jit812.Peer-Reviewed Original ResearchConceptsGenomic variationSingle nucleotide polymorphismsHigh-quality single nucleotide polymorphismsHigh-throughput sequencingInter-population differencesGenetic barcodesPlasmodium biologyGenetic variabilityGenomic dataInformative variantsDrug resistanceParasite populationsDifferential phenotypesLaboratory strainsMalaria parasitesNovel mutationsGlobal public health challengeNew insightsPf isolatesMalaria-endemic regionsPublic health challengePlasmodium falciparum speciesMedicine applicationsWeb-based resourcesClinical studies
2011
An Effective Method to Purify Plasmodium falciparum DNA Directly from Clinical Blood Samples for Whole Genome High-Throughput Sequencing
Auburn S, Campino S, Clark T, Djimde A, Zongo I, Pinches R, Manske M, Mangano V, Alcock D, Anastasi E, Maslen G, MacInnis B, Rockett K, Modiano D, Newbold C, Doumbo O, Ouédraogo J, Kwiatkowski D. An Effective Method to Purify Plasmodium falciparum DNA Directly from Clinical Blood Samples for Whole Genome High-Throughput Sequencing. PLOS ONE 2011, 6: e22213. PMID: 21789235, PMCID: PMC3138765, DOI: 10.1371/journal.pone.0022213.Peer-Reviewed Original ResearchPopulation Genetic Analysis of Plasmodium falciparum Parasites Using a Customized Illumina GoldenGate Genotyping Assay
Campino S, Auburn S, Kivinen K, Zongo I, Ouedraogo J, Mangano V, Djimde A, Doumbo O, Kiara S, Nzila A, Borrmann S, Marsh K, Michon P, Mueller I, Siba P, Jiang H, Su X, Amaratunga C, Socheat D, Fairhurst R, Imwong M, Anderson T, Nosten F, White N, Gwilliam R, Deloukas P, MacInnis B, Newbold C, Rockett K, Clark T, Kwiatkowski D. Population Genetic Analysis of Plasmodium falciparum Parasites Using a Customized Illumina GoldenGate Genotyping Assay. PLOS ONE 2011, 6: e20251. PMID: 21673999, PMCID: PMC3108946, DOI: 10.1371/journal.pone.0020251.Peer-Reviewed Original ResearchConceptsPopulation genetic studiesIllumina GoldenGateGenetic studiesSingle nucleotide polymorphism (SNP) discoveryLarge-scale population genetic studiesLaboratory clonesPopulation genetic diversityPopulation genetic analysesGenome sequencing projectsPlasmodium falciparum genomeTotal genomic DNAParasite population dynamicsMinor frequency allelesFalciparum genomePolymorphism discoveryGenetic diversitySequencing projectsWhole genomeP. falciparumGenetic analysisImportant phenotypesGenomic DNAHigh SNPPopulation dynamicsMultiple-clone infections
2003
Malaria: use of restriction endonuclease digestion and mutation-specific PCR for antifolate resistance isolate detection.
Kengne P, Ouédraogo J, Zampan H, Veas F, Guiguemdé T. Malaria: use of restriction endonuclease digestion and mutation-specific PCR for antifolate resistance isolate detection. Parassitologia 2003, 45: 27-31. PMID: 15270541.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsAntimalarialsDNA Mutational AnalysisDNA, ProtozoanDrug ResistanceFolic Acid AntagonistsHumansMalaria, FalciparumMutation, MissenseParasitemiaPlasmodium falciparumPoint MutationPolymerase Chain ReactionPolymorphism, Restriction Fragment LengthProguanilProtozoan ProteinsPyrimethamineTetrahydrofolate DehydrogenaseConceptsMutation-specific PCRCycloguanil resistanceBlood samplesResistance isolatesResistance mutationsPlasmodium falciparumMixed infectionsChemosensitivity analysisBloodWhole bloodParasite lysateRapid DNA extractionPCR productsRestriction endonuclease digestionPCRAmplified productsDry filter paperPCR technologyPoint mutationsEndonuclease digestionUndigested samplesPatientsInfectionPyrimethamineMutations