Molecular cloning and characterization of the Ehrlichia chaffeensis variable-length PCR target: an antigen-expressing gene that exhibits interstrain variation.
Sumner J, Childs J, Paddock C. Molecular cloning and characterization of the Ehrlichia chaffeensis variable-length PCR target: an antigen-expressing gene that exhibits interstrain variation. Journal Of Clinical Microbiology 1999, 37: 1447-53. PMID: 10203503, PMCID: PMC84798, DOI: 10.1128/jcm.37.5.1447-1453.1999.Peer-Reviewed Original ResearchConceptsOpen reading frameAmino acid sequenceApparent molecular massE. chaffeensisGenomic libraryMolecular cloningReading frameAcid sequenceDifferentiation of isolatesPCR targetsClose homologyMolecular massNucleotide sequencingImmunoreactive proteinDifferent isolatesDNA samplesInterstrain variationGenesRepeat unitsMolecular databasesCell culturesAmblyomma americanum ticksSequencePCR detectionChaffeensis