Hidden Mortality Attributable to Rocky Mountain Spotted Fever: Immunohistochemical Detection of Fatal, Serologically Unconfirmed Disease
Paddock C, Greer P, Ferebee T, Singleton J, McKechnie D, Treadwell T, Krebs J, Clarke M, Holman R, Olson J, Childs J, Zaki S. Hidden Mortality Attributable to Rocky Mountain Spotted Fever: Immunohistochemical Detection of Fatal, Serologically Unconfirmed Disease. The Journal Of Infectious Diseases 1999, 179: 1469-1476. PMID: 10228069, DOI: 10.1086/314776.Peer-Reviewed Original ResearchConceptsCase fatality ratioDiagnosis of RMSFIHC stainingRocky Mountain Spotted FeverLaboratory-confirmed casesAcute-phase seraTickborne infectionHidden mortalityIgM antibodiesDiagnostic titerPolymerase chain reactionIHC findingsPatient seraSerologic assaysImmunohistochemical stainingSpotted FeverImmunohistochemical detectionFatal diseasePatientsDisease controlIndirect immunofluorescenceNotifiable diseaseDiseaseUnderestimates of mortalityTissue samplesDiagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum
Comer J, Nicholson W, Sumner J, Olson J, Childs J. Diagnosis of Human Ehrlichiosis by PCR Assay of Acute-Phase Serum. Journal Of Clinical Microbiology 1999, 37: 31-34. PMID: 9854059, PMCID: PMC84159, DOI: 10.1128/jcm.37.1.31-34.1999.Peer-Reviewed Original ResearchConceptsDetermination of etiologyAcute-phase seraHuman granulocytic ehrlichiosisSerologic testingHuman ehrlichiosisAcute-phase serum samplesEarly detectionWhole blood specimensPCR assaysBlood specimensPCR testingEtiologic agentGranulocytic ehrlichiosisEtiologyHGE agentSimilar titersSerum samplesExposure historyTitersEhrlichia chaffeensisEhrlichiosisFourfold differenceE. chaffeensisSerumAntibodies