1999
Hepatic sequestration and modulation of the canalicular transport of the organic cation, daunorubicin, in the rat
Hayes J, Soroka C, Rios‐Velez L, Boyer J. Hepatic sequestration and modulation of the canalicular transport of the organic cation, daunorubicin, in the rat. Hepatology 1999, 29: 483-493. PMID: 9918926, DOI: 10.1002/hep.510290216.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnti-Bacterial AgentsAntineoplastic AgentsATP Binding Cassette Transporter, Subfamily B, Member 1BileBile CanaliculiBiological TransportBucladesineCationsChloroquineDaunorubicinKineticsLiverMacrolidesMaleMitochondrial ProteinsNocodazoleRatsRats, WistarRibosomal ProteinsSaccharomyces cerevisiae ProteinsTaurocholic AcidVerapamilConceptsBiliary excretionCoadministration of verapamilInhibitors of MDR1Rat hepatocyte coupletsBiliary recoveryDose administeredHepatic sequestrationTR- mutant ratsTherapeutic agentsExcretionDaunorubicinPericanalicular regionHepatocyte coupletsCanalicular transportRat liverBafilomycin ATaurocholateRatsIPRLIntracellular acidic compartmentsCellular localizationMicrotubule destabilizerConfocal microscopyIRHCPattern of distribution
1998
Nitric oxide and guanosine 3′,5′‐cyclic monophosphate stimulate bile secretion in isolated rat hepatocyte couplets, but not in isolated bile duct units
Trauner M, Mennone A, Gigliozzi A, Fraioli F, Boyer J. Nitric oxide and guanosine 3′,5′‐cyclic monophosphate stimulate bile secretion in isolated rat hepatocyte couplets, but not in isolated bile duct units. Hepatology 1998, 28: 1621-1628. PMID: 9828227, DOI: 10.1002/hep.510280623.Peer-Reviewed Original ResearchConceptsDuctular bile secretionBile secretionBile duct unitsNitric oxideRat hepatocyte coupletsExchanger activityNO synthesisCl-/HCO3Bile acid-independent bile flowCell-permeant cGMP analogDuct unitsEndogenous NO synthesisHepatocyte coupletsEffect of NOCell-permeant cAMP analogsLevel of hepatocytesCyclic monophosphateHepatic NO synthesisRemoval of HCO3Canalicular bile secretionNG-monomethylBile flowCholeretic effectDuctular luminaL-arginine
1995
Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets
Boyer J, Soroka C. Vesicle targeting to the apical domain regulates bile excretory function in isolated rat hepatocyte couplets. Gastroenterology 1995, 109: 1600-1611. PMID: 7557144, DOI: 10.1016/0016-5085(95)90649-5.Peer-Reviewed Original ResearchConceptsInsertion of vesiclesTranslocation of vesiclesMembrane transport activityVesicle trafficApical domainIsolated rat hepatocyte coupletsAcid transportersTransport activityNocodazoleRat hepatocyte coupletsVesiclesIndependent mechanismsEpithelial cellsBile acid transporterSite of residenceHepatocyte coupletsTransportersCanalicular membraneRat hepatocytesImmunocytochemical localizationCyclic monophosphateAdenosine triphosphataseBile excretory function
1991
The use of isolated rat hepatocyte couplets, microscopic optical planimetry and quantitative microfluorimetry in hepatobiliary physiology
Strazzabosco M, Boyer J, Crepaldi G. The use of isolated rat hepatocyte couplets, microscopic optical planimetry and quantitative microfluorimetry in hepatobiliary physiology. Cytotechnology 1991, 5: 88-89. PMID: 22358966, DOI: 10.1007/bf00736820.Peer-Reviewed Original ResearchEffect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers
Strazzabosco M, Sakisaka S, Hayakawa T, Boyer J. Effect of UDCA on intracellular and biliary pH in isolated rat hepatocyte couplets and perfused livers. American Journal Of Physiology 1991, 260: g58-g69. PMID: 1987808, DOI: 10.1152/ajpgi.1991.260.1.g58.Peer-Reviewed Original ResearchConceptsBiliary HCO3- concentrationUrsodeoxycholic acidRat hepatocyte coupletsBiliary pHAcid loadEffect of UDCABile duct lumenHepatocyte coupletsIsolated rat hepatocyte coupletsBiliary epitheliumHCO3- concentrationDuct lumenRemoval of ClHCO3- exchangeRat liverLiverIPRLHepatocytesHypercholeresisPerfusateBileExcretionEpitheliumAmiloride
1989
Voltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets
Weinman S, Graf J, Boyer J. Voltage-driven, taurocholate-dependent secretion in isolated hepatocyte couplets. American Journal Of Physiology 1989, 256: g826-g832. PMID: 2719107, DOI: 10.1152/ajpgi.1989.256.5.g826.Peer-Reviewed Original ResearchConceptsBile secretionBile acidsSecretion rateFluid secretionIntracellular current injectionCanalicular areaHepatocyte coupletsMembrane potentialRat hepatocyte coupletsAbsence of taurocholateMicroM taurocholateFluid secretion rateBile formationSecretionCarrier-mediated transportTaurocholateLiverIntracellular voltage
1988
Mechanisms of Bile Secretion: lnsights from the Isolated Rat Hepatocyte Couplet
Boyer J, Gautam A, Graf J. Mechanisms of Bile Secretion: lnsights from the Isolated Rat Hepatocyte Couplet. Seminars In Liver Disease 1988, 8: 308-316. PMID: 2850606, DOI: 10.1055/s-2008-1040552.Peer-Reviewed Original Research
1987
Na-H exchange regulates intracellular pH in isolated rat hepatocyte couplets
Henderson R, Graf J, Boyer J. Na-H exchange regulates intracellular pH in isolated rat hepatocyte couplets. American Journal Of Physiology 1987, 252: g109-g113. PMID: 3028151, DOI: 10.1152/ajpgi.1987.252.1.g109.Peer-Reviewed Original ResearchConceptsRat hepatocyte coupletsHepatocyte coupletsNa-H exchangeNa-H exchange mechanismOmission of sodiumBicarbonate-dependent systemsNominal absence