2007
Perspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor
Pike JW, Meyer MB, Watanuki M, Kim S, Zella LA, Fretz JA, Yamazaki M, Shevde NK. Perspectives on mechanisms of gene regulation by 1,25-dihydroxyvitamin D3 and its receptor. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 389-395. PMID: 17223545, PMCID: PMC1868541, DOI: 10.1016/j.jsbmb.2006.12.050.Peer-Reviewed Original ResearchConceptsTarget genesBasal transcriptional machineryChromatin immunoprecipitation techniqueVitamin D receptorKey target genesExpression of genesAltered gene expressionTranscriptional machineryVertebrate organismsGene regulationSystemic signalsTranscriptional modulationRegulatory regionsDNA sitesGene locusGene expressionPromoter regionEnhancer regionGenesImmunoprecipitation techniquesRegulatory capabilitiesMaintenance of calciumModular natureExpressionNew insightsMultiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3
Kim S, Yamazaki M, Zella LA, Meyer MB, Fretz JA, Shevde NK, Pike JW. Multiple enhancer regions located at significant distances upstream of the transcriptional start site mediate RANKL gene expression in response to 1,25-dihydroxyvitamin D3. The Journal Of Steroid Biochemistry And Molecular Biology 2007, 103: 430-434. PMID: 17197168, PMCID: PMC1892901, DOI: 10.1016/j.jsbmb.2006.12.020.Peer-Reviewed Original ResearchConceptsTranscription start siteStart siteChip analysisMultiple enhancer regionsRNA polymerase IIPotential regulatory regionsTranscriptional start siteRANKL geneVDR/RXRChromatin hubHistone modificationsTranscriptional outputPolymerase IIRANKL gene expressionImportant functional consequencesMultiple enhancersRegulatory regionsTranscription factorsHeterologous promoterGene locusEnhancer regionGene expressionKb upstreamRANKL promoterLuciferase assay
2006
1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription.
Fretz JA, Zella LA, Kim S, Shevde NK, Pike JW. 1,25-Dihydroxyvitamin D3 regulates the expression of low-density lipoprotein receptor-related protein 5 via deoxyribonucleic acid sequence elements located downstream of the start site of transcription. Endocrinology 2006, 20: 2215-30. PMID: 16613987, DOI: 10.1210/me.2006-0102.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone and BonesCells, CulturedChromatinChromatin ImmunoprecipitationConserved SequenceDimerizationDNAGene Expression RegulationHumansIntronsLDL-Receptor Related ProteinsLow Density Lipoprotein Receptor-Related Protein-5MiceMice, Inbred C57BLMolecular Sequence DataOsteoblastsPromoter Regions, GeneticProtein BindingReceptors, CalcitriolRetinoid X ReceptorsRNA Polymerase IISequence AlignmentTranscription, GeneticVitamin DVitamin D Response ElementConceptsVitamin D response elementD response elementResponse elementDNA microarray analysisChromatin structureChromatin immunoprecipitationOsteoblast biologyReceptor FrizzledHuman genomeLipoprotein receptor-related protein 5Regulatory regionsStart siteLrp5 locusLow-density lipoprotein receptor-related protein 5Sequence elementsHeterologous promoterMature osteoblastsMicroarray analysisOsteoblast precursorsDirect targetProtein 5Cell line originLine originPrimary osteoblastsGenes