2024
Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes
Yan Q, Gomis Perez C, Karatekin E. Cell Membrane Tension Gradients, Membrane Flows, and Cellular Processes. Physiology 2024, 39: 231-245. PMID: 38501962, PMCID: PMC11368524, DOI: 10.1152/physiol.00007.2024.Peer-Reviewed Original ResearchDetection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal resolution
Landajuela A, Braun M, Rodrigues C, Karatekin E. Detection of membrane fission in single Bacillus subtilis cells during endospore formation with high temporal resolution. STAR Protocols 2024, 5: 102965. PMID: 38502684, PMCID: PMC10963649, DOI: 10.1016/j.xpro.2024.102965.Peer-Reviewed Original ResearchMembrane fission during bacterial spore development requires cellular inflation driven by DNA translocation
Landajuela A, Braun M, Martínez-Calvo A, Rodrigues C, Perez C, Doan T, Rudner D, Wingreen N, Karatekin E. Membrane fission during bacterial spore development requires cellular inflation driven by DNA translocation. Biophysical Journal 2024, 123: 187a-188a. DOI: 10.1016/j.bpj.2023.11.1213.Peer-Reviewed Original ResearchRapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals
Perez C, Dudzinski N, Rouches M, Landajuela A, Machta B, Zenisek D, Karatekin E. Rapid propagation of membrane tension at retinal bipolar neuron presynaptic terminals. Biophysical Journal 2024, 123: 380a. DOI: 10.1016/j.bpj.2023.11.2322.Peer-Reviewed Original ResearchType IV secretion systems drive lipid mixing during substrate exchange
Chetrit D, Roy C, Karatekin E. Type IV secretion systems drive lipid mixing during substrate exchange. Biophysical Journal 2024, 123: 541a. DOI: 10.1016/j.bpj.2023.11.3276.Peer-Reviewed Original ResearchMechanisms of vesicle docking and fusion pore expansion by calcium-sensor synaptotagmin-1
Cheppali S, Tsemperouli M, Chetrit D, Rivera-Molina F, Toomre D, Karatekin E. Mechanisms of vesicle docking and fusion pore expansion by calcium-sensor synaptotagmin-1. Biophysical Journal 2024, 123: 381a. DOI: 10.1016/j.bpj.2023.11.2328.Peer-Reviewed Original Research
2023
PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection
Xu D, Jiang W, Wu L, Gaudet R, Park E, Su M, Cheppali S, Cheemarla N, Kumar P, Uchil P, Grover J, Foxman E, Brown C, Stansfeld P, Bewersdorf J, Mothes W, Karatekin E, Wilen C, MacMicking J. PLSCR1 is a cell-autonomous defence factor against SARS-CoV-2 infection. Nature 2023, 619: 819-827. PMID: 37438530, PMCID: PMC10371867, DOI: 10.1038/s41586-023-06322-y.Peer-Reviewed Original ResearchConceptsC-terminal β-barrel domainSpike-mediated fusionCell-autonomous defenseLarge-scale exome sequencingΒ-barrel domainGenome-wide CRISPRSARS-CoV-2 infectionHost cell cytosolScramblase activityPhospholipid scramblaseLive SARS-CoV-2 infectionHuman lung epitheliumPLSCR1SARS-CoV-2 USASingle-molecule switchingSARS-CoV-2 variantsExome sequencingHuman populationRestriction factorsViral RNANew SARS-CoV-2 variantsSARS-CoV-2Robust activityLung epitheliumDefense factorsLEMONADE: Lego-like membrane tension analyzer based on self-assembled DNA elastic networks
Yan Q, Liu L, Karatekin E, Lin C. LEMONADE: Lego-like membrane tension analyzer based on self-assembled DNA elastic networks. Biophysical Journal 2023, 122: 365a. DOI: 10.1016/j.bpj.2022.11.2014.Peer-Reviewed Original Research
2022
FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Landajuela A, Braun M, Rodrigues C, Martínez-Calvo A, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams N, Lin C, Wingreen N, Rudner D, Karatekin E. FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria. Biophysical Journal 2022, 121: 9a. DOI: 10.1016/j.bpj.2021.11.2658.Peer-Reviewed Original ResearchPolybasic patches in both C2 domains of synaptotagmin-1 are required for evoked neurotransmitter release
Wu Z, Tsemperouli M, Ma L, Courtney N, Zhu J, Zhang Y, Chapman E, Karatekin E. Polybasic patches in both C2 domains of synaptotagmin-1 are required for evoked neurotransmitter release. Biophysical Journal 2022, 121: 293a. DOI: 10.1016/j.bpj.2021.11.1283.Peer-Reviewed Original ResearchStepwise and cooperative membrane binding and tethering of extended synaptotagmins revealed by optical tweezers
Ge J, Bian X, Ma L, Cai Y, Li Y, Yang J, Karatekin E, De Camilli P, Zhang Y. Stepwise and cooperative membrane binding and tethering of extended synaptotagmins revealed by optical tweezers. Biophysical Journal 2022, 121: 313a. DOI: 10.1016/j.bpj.2021.11.1196.Peer-Reviewed Original ResearchRapid membrane flow at a presynaptic terminal
Perez C, Dudzinski N, Rouches M, Machta B, Zenisek D, Karatekin E. Rapid membrane flow at a presynaptic terminal. Biophysical Journal 2022, 121: 230a. DOI: 10.1016/j.bpj.2021.11.1599.Peer-Reviewed Original Research
2021
A human apolipoprotein L with detergent-like activity kills intracellular pathogens
Gaudet RG, Zhu S, Halder A, Kim BH, Bradfield CJ, Huang S, Xu D, Mamiñska A, Nguyen TN, Lazarou M, Karatekin E, Gupta K, MacMicking JD. A human apolipoprotein L with detergent-like activity kills intracellular pathogens. Science 2021, 373 PMID: 34437126, PMCID: PMC8422858, DOI: 10.1126/science.abf8113.Peer-Reviewed Original ResearchMeSH KeywordsApolipoproteins LBacterial Outer MembraneBacteriolysisCell MembraneCell Membrane PermeabilityCells, CulturedCRISPR-Cas SystemsCytosolDetergentsGene EditingGram-Negative BacteriaGTP-Binding ProteinsHumansImmunity, InnateInterferon-gammaLipoproteinsMicrobial ViabilityO AntigensProtein DomainsSalmonella typhimuriumSolubilityConceptsSingle-particle cryo-electron microscopyCell-autonomous defenseCytosol-invasive bacteriaExpression of hundredsNative mass spectrometryCryo-electron microscopyHuman genesDetergent-like activityHost proteinsLipoprotein nanodiscsMammalian lipidsExtracellular transportImmune cytokine interferonCell typesDetergent-like propertiesApolipoprotein LLife-threatening infectionsPotent bactericidal agentsAnionic membranesProteinCytokine interferonNonimmune cellsMass spectrometryCellsMutagenesisThe neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores
Wu Z, Dharan N, McDargh ZA, Thiyagarajan S, O'Shaughnessy B, Karatekin E. The neuronal calcium sensor Synaptotagmin-1 and SNARE proteins cooperate to dilate fusion pores. ELife 2021, 10: e68215. PMID: 34190041, PMCID: PMC8294851, DOI: 10.7554/elife.68215.Peer-Reviewed Original ResearchFisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria
Landajuela A, Braun M, Rodrigues CDA, Martínez-Calvo A, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams ND, Lin C, Wingreen NS, Rudner DZ, Karatekin E. FisB relies on homo-oligomerization and lipid binding to catalyze membrane fission in bacteria. PLOS Biology 2021, 19: e3001314. PMID: 34185788, PMCID: PMC8274934, DOI: 10.1371/journal.pbio.3001314.Peer-Reviewed Original ResearchConceptsMembrane fissionMembrane fission machineryMother cell membraneNegative-curvature lipidsMother cell cytoplasmUnique membrane topologyFission machineryMembrane scissionMembrane topologyLipid microdomainsMembrane necksFission proteinsLipid bindingMembrane curvatureFilamentous cellsBacillus subtilisFisBProtein BCell cytoplasmAcidic lipidsCell membraneCurvature sensingImmobile clustersIntrinsic abilityBacteriaSorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation
Yang Y, Wu Z, Wang L, Zhou K, Xia K, Xiong Q, Liu L, Zhang Z, Chapman ER, Xiong Y, Melia TJ, Karatekin E, Gu H, Lin C. Sorting sub-150-nm liposomes of distinct sizes by DNA-brick-assisted centrifugation. Nature Chemistry 2021, 13: 335-342. PMID: 33785892, PMCID: PMC8049973, DOI: 10.1038/s41557-021-00667-5.Peer-Reviewed Original ResearchThe Bacterial Membrane Fission Protein FisB Requires Homo-Oligomerization and Lipid-Binding to Catalyze Membrane Scission
Landajuela A, Braun M, Rodrigues C, Doan T, Horenkamp F, Andronicos A, Shteyn V, Williams N, Lin C, Rudner D, Karatekin E. The Bacterial Membrane Fission Protein FisB Requires Homo-Oligomerization and Lipid-Binding to Catalyze Membrane Scission. Biophysical Journal 2021, 120: 322a. DOI: 10.1016/j.bpj.2020.11.2033.Peer-Reviewed Original ResearchDynamics of Membrane Tension and Synaptic Vesicle Recycling
Perez C, Dudzinski N, Rouches M, Machta B, Zenisek D, Karatekin E. Dynamics of Membrane Tension and Synaptic Vesicle Recycling. Biophysical Journal 2021, 120: 323a. DOI: 10.1016/j.bpj.2020.11.2037.Peer-Reviewed Original Research
2020
DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells
Williams ND, Landajuela A, Kasula RK, Zhou W, Powell JT, Xi Z, Isaacs FJ, Berro J, Toomre D, Karatekin E, Lin C. DNA-Origami-Based Fluorescence Brightness Standards for Convenient and Fast Protein Counting in Live Cells. Nano Letters 2020, 20: 8890-8896. PMID: 33164530, PMCID: PMC7726105, DOI: 10.1021/acs.nanolett.0c03925.Peer-Reviewed Original ResearchConceptsCopies of proteinsBrightness standardsFluorescence microscopyProtein countingMammalian cellsFluorescent proteinCopy numberLive cellsConventional quantification techniquesBiological LaboratoryFluorescence microscopeProteinDNA origamiVersatile toolCellsBiologyCurrent quantification methodsCopiesOrganic dyesRetromer forms low order oligomers on supported lipid bilayers
Deatherage CL, Nikolaus J, Karatekin E, Burd CG. Retromer forms low order oligomers on supported lipid bilayers. Journal Of Biological Chemistry 2020, 295: 12305-12316. PMID: 32651229, PMCID: PMC7443500, DOI: 10.1074/jbc.ra120.013672.Peer-Reviewed Original ResearchConceptsLow-order oligomersAccessory factorsLipid bilayersMembrane-associated cargoesCoat protein complexIntegral membrane proteinsModel cargo proteinSingle-particle fluorescence microscopyRetromer sorting pathwayMembrane cargoNexin 3WASH complexCargo proteinsSorting pathwaysMembrane associationProtein complexesSNX3-retromerEndosomal systemMembrane proteinsEndosome membraneRecycling pathwayRetromerOligomeric interactionsIntrinsic propensityFluorescence microscopy