2021
Obtaining Xenopus tropicalis Eggs.
Lane M, Mis EK, Khokha MK. Obtaining Xenopus tropicalis Eggs. Cold Spring Harbor Protocols 2021, 2022: pdb.prot106344. PMID: 34031209, DOI: 10.1101/pdb.prot106344.Peer-Reviewed Original ResearchConceptsDevelopmental biologyGene manipulation toolsPowerful model systemCell biological studiesCell-free systemTetraploid genomeDiploid genomeThousands of eggsEgg extractsGenetic studiesXenopusGenomePremier systemModel systemEggsBiological studiesBiologyEmbryosFrogsManipulation toolsTiming of stepsSpeciesHormoneFemalesCells
2015
CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo
Moreno-Mateos MA, Vejnar CE, Beaudoin JD, Fernandez JP, Mis EK, Khokha MK, Giraldez AJ. CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Nature Methods 2015, 12: 982-988. PMID: 26322839, PMCID: PMC4589495, DOI: 10.1038/nmeth.3543.Peer-Reviewed Original Research
2014
Uncovering Buffered Pleiotropy: A Genome-Scale Screen for mel-28 Genetic Interactors in Caenorhabditis elegans
Fernandez A, Mis E, Lai A, Mauro M, Quental A, Bock C, Piano F. Uncovering Buffered Pleiotropy: A Genome-Scale Screen for mel-28 Genetic Interactors in Caenorhabditis elegans. G3: Genes, Genomes, Genetics 2014, 4: 185-196. PMID: 24281427, PMCID: PMC3887534, DOI: 10.1534/g3.113.008532.Peer-Reviewed Original ResearchConceptsMEL-28Caenorhabditis elegansLethal genesGenetic interaction screensGenome-scale screeningWild-type wormsNuclear envelope functionsCell-matrix attachmentWild-type larvaeDynactin functionGenetic interactorsGenome-scaleVesicle transportConserved proteinsChromosome segregationGene networksIdentified genesCellular processesPostembryonic rolesInteraction screenNuclear poresPleiotropic functionsGenesEarly embryosLarval development