2017
High concordance of a closed-system, RT-qPCR breast cancer assay for HER2 mRNA, compared to clinically determined immunohistochemistry, fluorescence in situ hybridization, and quantitative immunofluorescence
Wasserman BE, Carvajal-Hausdorf DE, Ho K, Wong W, Wu N, Chu VC, Lai EW, Weidler JM, Bates M, Neumeister V, Rimm DL. High concordance of a closed-system, RT-qPCR breast cancer assay for HER2 mRNA, compared to clinically determined immunohistochemistry, fluorescence in situ hybridization, and quantitative immunofluorescence. Laboratory Investigation 2017, 97: 1521-1526. PMID: 28892092, PMCID: PMC5711560, DOI: 10.1038/labinvest.2017.93.Peer-Reviewed Original ResearchConceptsInvasive breast cancerBreast cancerQuantitative immunofluorescenceRT-qPCRFormalin-fixed paraffin-embedded tissue blocksRT-qPCR assaysEquivocal categoryReal-time quantitative reverse transcription polymerase chain reactionHER2 receptor statusQuantitative reverse transcription polymerase chain reactionParaffin-embedded tissue blocksReverse transcription-polymerase chain reactionTranscription-polymerase chain reactionIHC/FISHMRNA measurementsAssessment of HER2Low-resource settingsReceptor statusPolymerase chain reactionPathology reportsCT cutsSingle-use cartridgeResource settingsHER2 mRNATissue blocks
2011
PD05-04: Quantitative Measurement of Antigen Degradation in NCCTG N9831 Tissue Microarrays.
Cheng H, Rimm D, Reinholz M, Lingle W, Ballman K, Dueck A, Chen B, McCullough A, Jenkins R, Perez E. PD05-04: Quantitative Measurement of Antigen Degradation in NCCTG N9831 Tissue Microarrays. Cancer Research 2011, 71: pd05-04-pd05-04. DOI: 10.1158/0008-5472.sabcs11-pd05-04.Peer-Reviewed Original ResearchCooperative group studiesTissue microarrayAntigen degradationAdjuvant phase III trialsPhase III trialsGroup studyExpression of HER2Paraffin-embedded tissuesIII trialsEntire cohortExpression of HER1Analysis of biomarkersPositive casesHER2Quantitative immunofluorescenceTumor samplesCancer ResHER2 scoreTissue blocksOld casesTissue collectionAverage scoreHER1TMA slidesPre-analytical variables