2011
Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae
Roelants FM, Breslow DK, Muir A, Weissman JS, Thorner J. Protein kinase Ypk1 phosphorylates regulatory proteins Orm1 and Orm2 to control sphingolipid homeostasis in Saccharomyces cerevisiae. Proceedings Of The National Academy Of Sciences Of The United States Of America 2011, 108: 19222-19227. PMID: 22080611, PMCID: PMC3228448, DOI: 10.1073/pnas.1116948108.Peer-Reviewed Original ResearchConceptsProtein kinase Ypk1Orm phosphorylationSphingolipid depletionSphingolipid biosynthesisTORC2-dependent phosphorylationTemperature-sensitive growthIntegral membrane proteinsWild-type levelsOnly weak activationPrimary physiological roleMutant displaysYpk1Regulatory modificationFamily proteinsMembrane proteinsORM geneKey homeostatic regulatorsProtein kinaseSphingolipid homeostasisFirst enzymeSphingolipid productionSphingolipid synthesisEndoplasmic reticulumPhysiological rolePhosphorylation
2010
Membranes in Balance: Mechanisms of Sphingolipid Homeostasis
Breslow DK, Weissman JS. Membranes in Balance: Mechanisms of Sphingolipid Homeostasis. Molecular Cell 2010, 40: 267-279. PMID: 20965421, PMCID: PMC2987644, DOI: 10.1016/j.molcel.2010.10.005.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsConceptsSphingolipid homeostasisCell biologyEukaryotic cell biologyKey cellular rolesComplex membrane compositionCellular rolesSecretory pathwaySphingolipid biosynthesisEnzymatic machineryPhysiologic cuesSphingolipid metabolismMembrane compositionSphingolipidsBiologyNew insightsHomeostasisStructural componentsMembraneCellsBiosynthesisDefining featureMachineryGlycerolipidsEnzymeImproved understanding