2021
Macrophage-derived PDGF-B induces muscularization in murine and human pulmonary hypertension
Ntokou A, Dave JM, Kauffman AC, Sauler M, Ryu C, Hwa J, Herzog EL, Singh I, Saltzman WM, Greif DM. Macrophage-derived PDGF-B induces muscularization in murine and human pulmonary hypertension. JCI Insight 2021, 6: e139067. PMID: 33591958, PMCID: PMC8026182, DOI: 10.1172/jci.insight.139067.Peer-Reviewed Original ResearchConceptsRight ventricle hypertrophyPulmonary hypertensionDistal muscularizationSmooth muscle cellsHypoxia-inducible factor 2APulmonary arterial hypertension patientsLysM-Cre miceArterial hypertension patientsHuman pulmonary hypertensionHypoxia-inducible factor 1aPlatelet-derived growth factorMacrophage-conditioned mediumVentricle hypertrophyHypertension patientsDistal arteriolesMacrophage accumulationFl miceCardiovascular diseaseInterventional strategiesMuscularizationHypoxia exposureExcess macrophagesSMC proliferationLethal diseaseMuscle cells
2018
NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy
Dubrac A, Künzel SE, Künzel SH, Li J, Chandran RR, Martin K, Greif DM, Adams RH, Eichmann A. NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nature Communications 2018, 9: 3463. PMID: 30150707, PMCID: PMC6110853, DOI: 10.1038/s41467-018-05926-7.Peer-Reviewed Original ResearchConceptsNeovascular tuftsPericyte migrationMural cellsPathological neovascular tuftsPDGF-B overexpressionTreatment of retinopathyOxygen-induced retinopathyCapillary barrier functionΑ-SMA expressionPlatelet-derived growth factor receptorPlatelet-derived growth factorPhosphorylation of PDGFRβGrowth factor receptorIschemic retinopathyProliferative retinopathyEndothelial-specific knockoutVascular leakagePericyte depletionPathological neovascularizationGenetic lineage tracingMouse modelPericyte proliferationNck2 adaptor proteinRetinopathyBarrier function