1999
Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein.
Naeger L, Goodwin E, Hwang E, DeFilippis R, Zhang H, DiMaio D. Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein. Molecular Cancer Research 1999, 10: 413-22. PMID: 10392903.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCattleCdc25 PhosphatasesCDC2-CDC28 KinasesCell Cycle ProteinsCell DivisionCyclin ACyclin-Dependent Kinase 2Cyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent KinasesCyclinsDNA-Binding ProteinsE2F Transcription FactorsE2F1 Transcription FactorEnzyme InhibitorsFemaleGene Expression RegulationGrowth InhibitorsHeLa CellsHumansPhosphoprotein PhosphatasesPhosphorylationProtein Serine-Threonine KinasesProtein Tyrosine PhosphatasesRepressor ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Transcription Factor DP1Transcription FactorsTumor Cells, CulturedTumor Suppressor Protein p53Uterine Cervical NeoplasmsViral ProteinsConceptsCervical carcinoma cell linesHT-3 cellsCarcinoma cell linesE2 proteinE6/E7 genesCell linesGrowth inhibitionCervical carcinoma cellsCyclin ACyclin-dependent kinase 2 activityExpression of CDC25AKinase 2 activityE7 genesCell cycle componentsCarcinoma cellsCDC25B expressionE2 expressionProtein levels
1989
Structure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product
Horwitz B, Settleman J, Prakash S, DiMaio D. Structure, Activity, and Regulation of the Bovine Papillomavirus E5 Gene and Its Transforming Protein Product. Current Topics In Microbiology And Immunology 1989, 144: 143-151. PMID: 2551579, DOI: 10.1007/978-3-642-74578-2_18.Peer-Reviewed Original ResearchConceptsE5 geneFoci formationBovine papillomavirus type 1 DNACell focus formationBPV geneCodon resultsC127 cellsMouse cellsTumorigenic transformationProtein productsK polypeptideGenesViral mutantsBiochemical analysisEfficient transformationCellsMutantsPolypeptideDNARegulationExpressionDownstreamActivityTranslationFormation
1988
Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus.
Settleman J, DiMaio D. Efficient transactivation and morphologic transformation by bovine papillomavirus genes expressed from a bovine papillomavirus/simian virus 40 recombinant virus. Proceedings Of The National Academy Of Sciences Of The United States Of America 1988, 85: 9007-9011. PMID: 2848252, PMCID: PMC282651, DOI: 10.1073/pnas.85.23.9007.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1 E2Simian virus 40 early regionTransactivation of genesCultured mammalian cellsHybrid viral genomesSimian virus 40 virionsMouse C127 cellsSimian virus 40 large tumor antigenBovine papillomavirus type 1Large tumor antigenPapillomavirus type 1E5 mutantsAcute cellular responseRegulatory genesMammalian cellsControl regionEfficient transactivationForeign genesReporter geneType 1 geneWild-type virusC127 cellsE5 geneTumorigenic transformationCellular responsesBovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene
Prakash S, Horwitz B, Zibello T, Settleman J, DiMaio D. Bovine papillomavirus E2 gene regulates expression of the viral E5 transforming gene. Journal Of Virology 1988, 62: 3608-3613. PMID: 2843663, PMCID: PMC253501, DOI: 10.1128/jvi.62.10.3608-3613.1988.Peer-Reviewed Original ResearchAcetyltransferasesAmino Acid SequenceAnimalsBase SequenceBovine papillomavirus 1Cell LineCell Transformation, ViralChloramphenicol O-AcetyltransferaseDNA-Binding ProteinsGene Expression RegulationGenes, ViralMolecular Sequence DataMutationOncogene Proteins, ViralOncogenesPapillomaviridaePlasmidsPrecipitin TestsPromoter Regions, GeneticTransfectionViral ProteinsBovine papillomavirus mutant temperature sensitive for transformation, replication and transactivation.
DiMaio D, Settleman J. Bovine papillomavirus mutant temperature sensitive for transformation, replication and transactivation. The EMBO Journal 1988, 7: 1197-1204. PMID: 2841117, PMCID: PMC454456, DOI: 10.1002/j.1460-2075.1988.tb02931.x.Peer-Reviewed Original ResearchConceptsMutant viral DNANormal viral DNA replicationMajor viral regulatory proteinLack of mutantsN-terminal halfTemperature-sensitive defectMouse C127 cellsViral DNA replicationViral regulatory proteinsStrong genetic evidenceViral DNAPleiotropic phenotypesGenetic experimentsMost cell linesDNA replicationGene activityGenetic evidenceReplication defectRegulatory proteinsExtrachromosomal plasmidsMutant temperatureC127 cellsTemperature shiftGenetic analysisViral biological activities
1983
Identification of two distinct regulatory regions adjacent to the human β-interferon gene
Zinn K, Dimaio D, Maniatis T. Identification of two distinct regulatory regions adjacent to the human β-interferon gene. Cell 1983, 34: 865-879. PMID: 6313211, DOI: 10.1016/0092-8674(83)90544-5.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceBovine papillomavirus 1Cell Transformation, ViralChromosome DeletionDNAGene Expression RegulationHumansInterferon Type IMutationPoly I-CRNA, MessengerConceptsIFN gene expressionBovine papilloma virusRegulatory regionsExpression of deletion mutantsGene expressionMRNA cap sitePoly(I)-poly(CCell linesStable cell linesIndependent cell linesCloned geneStructural geneDeletion mutantsLevel of expressionKinetics of inductionCap siteMouse cellsHuman beta-interferonGenesInduced expressionConstitutive levelsDeletionBeta-interferonExpressionPapilloma virus
1982
Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes
DiMaio D, Nathans D. Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. Journal Of Molecular Biology 1982, 156: 531-548. PMID: 6288959, DOI: 10.1016/0022-2836(82)90265-0.Peer-Reviewed Original ResearchConceptsViral DNA replicationDNA replicationEarly gene transcriptionT antigenGene transcriptionGene expressionCold-sensitive defectSecond-site mutationsSegment of DNAT antigen functionLate protein synthesisSimian virus 40 mutantsSV40 early proteinsLarge T antigenT-antigen geneEarly gene expressionBase pair substitutionsRegulatory mutantsRegulatory segmentRegulatory regionsDefined deletionsViral regulatory regionViral genesMutantsDNA show
1981
Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants
McKay R, DiMaio D. Binding of an SV40 T antigen-related protein to the DNA of SV40 regulatory mutants. Nature 1981, 289: 810-813. PMID: 6258090, DOI: 10.1038/289810a0.Peer-Reviewed Original Research