2020
Cell-penetrating peptide inhibits retromer-mediated human papillomavirus trafficking during virus entry
Zhang P, Moreno R, Lambert PF, DiMaio D. Cell-penetrating peptide inhibits retromer-mediated human papillomavirus trafficking during virus entry. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 6121-6128. PMID: 32123072, PMCID: PMC7084110, DOI: 10.1073/pnas.1917748117.Peer-Reviewed Original ResearchConceptsEssential protein-protein interactionsCellular protein complexesProtein-protein interactionsIntracellular virus traffickingRetrograde transport pathwaySites of replicationCell-penetrating sequenceProtein complexesCellular proteinsVirus replicationHPV16 pseudovirus infectionVirus traffickingL2 capsid proteinsAspects of infectionCapsid proteinHPV entryViral genomeViral proteinsIncoming virionsViral componentsHuman papillomavirus infectionProteinAntiviral targetDose-dependent blockVirus entry
1990
Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity
Riese D, Settleman J, Neary K, DiMaio D. Bovine papillomavirus E2 repressor mutant displays a high-copy-number phenotype and enhanced transforming activity. Journal Of Virology 1990, 64: 944-949. PMID: 2153255, PMCID: PMC249196, DOI: 10.1128/jvi.64.2.944-949.1990.Peer-Reviewed Original ResearchConceptsRepressor proteinWild-type copy numberBovine papillomavirus type 1 genomeCopy numberViral genomeTranscriptional repressor proteinTransient expression experimentsMouse C127 cellsMutant viral DNAViral DNAType 1 genomeNumber phenotypeRepressor activityLow copy numberMethionine codonInitiation codonExpression experimentsC127 cellsGenomeCV1 cellsRepressor mutantsFoci formationColony formationCodonProtein
1989
Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells
Neary K, DiMaio D. Open reading frames E6 and E7 of bovine papillomavirus type 1 are both required for full transformation of mouse C127 cells. Journal Of Virology 1989, 63: 259-266. PMID: 2535732, PMCID: PMC247680, DOI: 10.1128/jvi.63.1.259-266.1989.Peer-Reviewed Original ResearchConceptsBovine papillomavirus type 1Open reading frames E6Mouse C127 cellsFull-length viral genomesAnchorage-independent growthPapillomavirus type 1Focus-forming activityC127 cellsORF E6First methionine codonViral genomeColony formationRetrovirus long terminal repeatsSeries of mutationsE6/E7Second ATG codonLong terminal repeatBPV1 genomeMethionine codonATG codonNumber plasmidE5 geneSpecific proteinsSimultaneous disruptionE6 protein
1987
Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein.
Burkhardt A, DiMaio D, Schlegel R. Genetic and biochemical definition of the bovine papillomavirus E5 transforming protein. The EMBO Journal 1987, 6: 2381-2385. PMID: 2822390, PMCID: PMC553643, DOI: 10.1002/j.1460-2075.1987.tb02515.x.Peer-Reviewed Original ResearchConceptsFirst methionine codonMethionine codonE5 proteinCellular membranesAmino-terminal mutationsFrameshift mutationBovine papillomavirus E5Mutant viral genomesCellular transformationInitiation codonHydrophobic polypeptidesBPV genomeSmall polypeptidesCell transformationCodonViral genomeE5 ORFProteinBovine papillomavirusGenetic alterationsMutationsPolypeptideForm dimersGenomeFrame mutations
1979
Constructed Mutants of Simian Virus 40
Shortle D, Pipas J, Lazarowitz S, DiMaio D, Nathans D. Constructed Mutants of Simian Virus 40. Genetic Engineering: Principles And Methods 1979, 73-92. DOI: 10.1007/978-1-4615-7072-1_5.Peer-Reviewed Original ResearchGenetic analysisDNA moleculesNucleic acid biochemistryRegulatory elementsSimian virus 40Such mutantsDNA sequencesNucleotide sequenceRandom mutagenesisPhenotype selectionGenomePhysiological defectsSpontaneous mutationsMutantsSpecific base changesViral genomeBase changesRestriction endonucleasesRestriction sitesMutant virusCleavage mapSite specificitySpecific sitesSequenceRecent advances