1989
Genetic Evidence that Acute Morphologic Transformation, Induction of Cellular DNA Synthesis, and Focus Formation Are Mediated by a Single Activity of the Bovine Papillomavirus E5 Protein
Settleman J, Fazeli A, Malicki J, Horwitz B, Dimaio D. Genetic Evidence that Acute Morphologic Transformation, Induction of Cellular DNA Synthesis, and Focus Formation Are Mediated by a Single Activity of the Bovine Papillomavirus E5 Protein. Molecular And Cellular Biology 1989, 9: 5563-5572. DOI: 10.1128/mcb.9.12.5563-5572.1989.Peer-Reviewed Original ResearchE5 proteinE5 geneCellular DNA synthesisC127 cellsBovine papillomavirus E5 proteinMouse C127 cellsDNA synthesisMorphologic transformationCultured rodent cellsDefective phenotypeMissense mutantsUnstable proteinDefective mutantsGenetic evidenceMutational analysisE5 activityRodent cellsCell cycleViral genesBiochemical activitySerum starvationFoci formationCell transformationGenesContact inhibitionGenetic evidence that acute morphologic transformation, induction of cellular DNA synthesis, and focus formation are mediated by a single activity of the bovine papillomavirus E5 protein.
Settleman J, Fazeli A, Malicki J, Horwitz B, DiMaio D. Genetic evidence that acute morphologic transformation, induction of cellular DNA synthesis, and focus formation are mediated by a single activity of the bovine papillomavirus E5 protein. Molecular And Cellular Biology 1989, 9: 5563-5572. PMID: 2555701, PMCID: PMC363726, DOI: 10.1128/mcb.9.12.5563.Peer-Reviewed Original ResearchConceptsE5 proteinE5 geneCellular DNA synthesisC127 cellsBovine papillomavirus E5 proteinMouse C127 cellsDNA synthesisMorphologic transformationCultured rodent cellsDefective phenotypeMissense mutantsUnstable proteinDefective mutantsGenetic evidenceMutational analysisE5 activityRodent cellsCell cycleViral genesBiochemical activitySerum starvationCell transformationGenesContact inhibitionVirus multiplicityGenetic Evidence that Acute Morphologic Transformation, Induction of Cellular DNA Synthesis, and Focus Formation Are Mediated by a Single Activity of the Bovine Papillomavirus E5 Protein
Settleman J, Fazeli A, Malicki J, Horwitz B, Dimaio D. Genetic Evidence that Acute Morphologic Transformation, Induction of Cellular DNA Synthesis, and Focus Formation Are Mediated by a Single Activity of the Bovine Papillomavirus E5 Protein. Molecular And Cellular Biology 1989, 9: 5563-5572. DOI: 10.1128/mcb.9.12.5563-5572.1989.Peer-Reviewed Original ResearchInduction of cellular DNA synthesisE5 proteinCellular DNA synthesisBovine papillomavirusC127 cellsBovine papillomavirus E5 proteinDNA synthesisStable cell transformantsMouse C127 cellsMutants expressed normal levelsVirus multiplicity of infectionMissense mutantsDefective phenotypesGenetic evidenceE5 geneE5 activitySerum starvationFocus-forming assayMultiplicity of infectionCell cycleMutation analysisViral genesBiochemical activityMorphological transformationContact inhibition
1982
Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes
DiMaio D, Nathans D. Regulatory mutants of simian virus 40 Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes. Journal Of Molecular Biology 1982, 156: 531-548. PMID: 6288959, DOI: 10.1016/0022-2836(82)90265-0.Peer-Reviewed Original ResearchConceptsViral DNA replicationDNA replicationEarly gene transcriptionT antigenGene transcriptionGene expressionCold-sensitive defectSecond-site mutationsSegment of DNAT antigen functionLate protein synthesisSimian virus 40 mutantsSV40 early proteinsLarge T antigenT-antigen geneEarly gene expressionBase pair substitutionsRegulatory mutantsRegulatory segmentRegulatory regionsDefined deletionsViral regulatory regionViral genesMutantsDNA show