An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity
Li D, Yang J, Malik V, Huang Y, Huang X, Zhou H, Wang J. An RNAi screen of RNA helicases identifies eIF4A3 as a regulator of embryonic stem cell identity. Nucleic Acids Research 2022, 50: 12462-12479. PMID: 36416264, PMCID: PMC9757061, DOI: 10.1093/nar/gkac1084.Peer-Reviewed Original ResearchMeSH KeywordsDEAD-box RNA HelicasesEmbryonic Stem CellsEukaryotic Initiation Factor-4ARNA InterferenceRNA, MessengerConceptsExon junction complexRNA helicaseRNA interferenceEmbryonic stem cellsPost-transcriptionallyEmbryonic stem cell identityEfficient nuclear exportRNA interference screenCell cycle progression of ESCsPost-transcriptional controlCell cycle regulationStem cell identityCell cycle progressionMaintenance of embryonic stem cellsStem cell pluripotencyLoss of pluripotencyRNA metabolismDEAD-boxCell cycle dysregulationCell identityCycle regulationCell pluripotencyCell cyclePluripotency controlCyclin B1Comparative functional genomics identifies unique molecular features of EPSCs
Malik V, Zang R, Fuentes-Iglesias A, Huang X, Li D, Fidalgo M, Zhou H, Wang J. Comparative functional genomics identifies unique molecular features of EPSCs. Life Science Alliance 2022, 5: e202201608. PMID: 35961778, PMCID: PMC9378845, DOI: 10.26508/lsa.202201608.Peer-Reviewed Original ResearchConceptsEmbryonic stem cellsProteome of embryonic stem cellsComparative functional genomicsHistone modification marksChromatin accessibility featuresModification marksChromatin accessibilityFunctional genomicsRegulatory networksTranslational regulationPluripotency factors OCT4Molecular foundationMaintenance in vitroMolecular underpinningsTotipotent stem cellsMolecular signaturesChromatinFactors Oct4Stem cellsDevelopmental potencySelf-renewalMolecular featuresGenomeCellsTranscriptome