2024
Decidual Cells Block Inflammation-Mediated Inhibition of 15-Hydroxyprostaglandin Dehydrogenase in Trophoblasts
de Assis V, Kayisli U, Ozmen A, Semerci N, Totary-Jain H, Pakasticali N, Halade G, Lockwood C, Guzeloglu-Kayisli O. Decidual Cells Block Inflammation-Mediated Inhibition of 15-Hydroxyprostaglandin Dehydrogenase in Trophoblasts. American Journal Of Pathology 2024, 194: 1724-1736. PMID: 38879084, DOI: 10.1016/j.ajpath.2024.05.005.Peer-Reviewed Original ResearchConditioned medium supernatantTerm decidual cellsDecidual cellsExtravillous trophoblastHPGD expressionGestational age-matched controlsHuman trophoblast stem cellsFetal membrane ruptureMaternal-fetal interfaceTrophoblast stem cellsAge-matched controlsDecidua parietalisCervical ripeningUterine quiescencePreterm birthUterine contractionsAnchoring villiPrimary trophoblastsParacrine mechanismsParacrine inhibitionPregnancy maintenanceIL-1BInactivate prostaglandinsStem cellsProstaglandin
2009
Progestin and Thrombin Regulate Tissue Factor Expression in Human Term Decidual Cells
Lockwood CJ, Murk W, Kayisli UA, Buchwalder LF, Huang S, Funai EF, Krikun G, Schatz F. Progestin and Thrombin Regulate Tissue Factor Expression in Human Term Decidual Cells. The Journal Of Clinical Endocrinology & Metabolism 2009, 94: 2164-2170. PMID: 19276228, PMCID: PMC2690421, DOI: 10.1210/jc.2009-0065.Peer-Reviewed Original ResearchConceptsMaternal-fetal interfaceTerm decidual cellsDecidual cellsTNF-alphaTissue factorPlacental sectionsTF mRNA levelsIL-1betaHuman maternal-fetal interfaceTF expressionWestern blottingTF levelsVillous mesenchymal cellsRT-PCRTerm placental sectionsMRNA levelsHigher histologic scoresTissue factor expressionIL-1beta effectReal-time RT-PCRQuantitative real-time RT-PCRDecidual sectionsHemostatic protectionPreterm deliveryQuantitative RT-PCR
2008
Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells
Lockwood CJ, Yen CF, Basar M, Kayisli UA, Martel M, Buhimschi I, Buhimschi C, Huang SJ, Krikun G, Schatz F. Preeclampsia-Related Inflammatory Cytokines Regulate Interleukin-6 Expression in Human Decidual Cells. American Journal Of Pathology 2008, 172: 1571-1579. PMID: 18467705, PMCID: PMC2408417, DOI: 10.2353/ajpath.2008.070629.Peer-Reviewed Original ResearchConceptsIL-6 levelsIL-6Decidual cellsGestational age-matched control placentasElevated IL-6 levelsIL-6 mRNA levelsCommon pregnancy disorderGenesis of preeclampsiaUteroplacental blood flowFetal-placental unitInterleukin-6 levelsPathogenesis of preeclampsiaPro-inflammatory cytokinesEndothelial cell dysfunctionInterleukin-6 expressionHuman decidual cellsReal-time quantitative RT-PCRTime quantitative RT-PCRQuantitative RT-PCRFetal morbidityDecidual macrophagesPreeclamptic womenSystemic inflammationPregnancy disordersControl placentasDecidual Cell‐expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia‐related Angiogenesis
Lockwood CJ, Krikun G, Caze R, Rahman M, Buchwalder LF, Schatz F. Decidual Cell‐expressed Tissue Factor in Human Pregnancy and Its Involvement in Hemostasis and Preeclampsia‐related Angiogenesis. Annals Of The New York Academy Of Sciences 2008, 1127: 67-72. PMID: 18443332, DOI: 10.1196/annals.1434.013.Peer-Reviewed Original ResearchConceptsDecidual cell-expressed tissue factorUteroplacental blood flowVascular endothelial growth factorTissue factorDecidual cellsBlood flowFirst trimester decidual cellsGrowth factorPlacental growth factorProtease-activated receptorsSFlt-1 expressionEndothelial growth factorPredominant cell typeMaternal thrombophiliaMaternal morbidityDecidual hemorrhageSpiral arteriesHuman pregnancySFlt-1Trophoblast invasionVascular remodelingPreeclampsiaVEGF expressionExcess thrombinAngiogenic effectsMatrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells1
Lockwood CJ, Oner C, Uz YH, Kayisli UA, Huang SJ, Buchwalder LF, Murk W, Funai EF, Schatz F. Matrix Metalloproteinase 9 (MMP9) Expression in Preeclamptic Decidua and MMP9 Induction by Tumor Necrosis Factor Alpha and Interleukin 1 Beta in Human First Trimester Decidual Cells1. Biology Of Reproduction 2008, 78: 1064-1072. PMID: 18276934, PMCID: PMC3045968, DOI: 10.1095/biolreprod.107.063743.Peer-Reviewed Original ResearchMeSH KeywordsBase SequenceCase-Control StudiesCells, CulturedDeciduaDNA PrimersEnzyme InductionFemaleHumansImmunohistochemistryInterleukin-1betaMatrix Metalloproteinase 2Matrix Metalloproteinase 9Pre-EclampsiaPregnancyPregnancy Trimester, FirstReverse Transcriptase Polymerase Chain ReactionRNA, MessengerTissue Inhibitor of Metalloproteinase-1Tissue Inhibitor of Metalloproteinase-2Tumor Necrosis Factor-alphaConceptsTumor necrosis factor alphaNecrosis factor alphaInterleukin-1 betaMedroxyprogesterone acetateFactor alphaDecidual cellsMMP9 levelsExtravillous trophoblastsEVT invasionFirst trimester decidual cellsAge-matched control womenMatrix metalloproteinase-9 expressionUteroplacental blood flowLevels of MMP9Metalloproteinase-9 expressionMatrix metalloproteinases 1Matrix metalloproteinases 2Levels of MMP2Decidual extracellular matrixConcentration-dependent increaseSubstrate gel zymographyPreeclamptic deciduaQuantitative RT-PCRInterstitial trophoblastSpiral arteries
1998
Apoptosis and Fas Expression in Human Fetal Membranes1
Runić R, Lockwood C, LaChapelle L, Dipasquale B, Demopoulos R, Kumar A, Guller S. Apoptosis and Fas Expression in Human Fetal Membranes1. The Journal Of Clinical Endocrinology & Metabolism 1998, 83: 660-666. PMID: 9467589, DOI: 10.1210/jcem.83.2.4600.Peer-Reviewed Original ResearchConceptsChorionic trophoblastsHuman fetal membranesFetal membranesApoptotic indexWeeks groupCell layerHuman fetal membrane cellsCytoplasmic domain of FasFetal membrane cellsFas messenger ribonucleic acidTrophoblast cell layerDecidual cell typesCondensation of chromatinConsistent with apoptosisSamples of amnionEpithelial cell layerMessenger ribonucleic acidCell surface receptorsWeeks gestationDecidual cellsProstate glandAmnion epitheliumFas signalingAmniotic tissueImmunohistochemical staining