Implications of macromolecular crowding for signal transduction and metabolite channeling
Rohwer J, Postma P, Kholodenko B, Westerhoff H. Implications of macromolecular crowding for signal transduction and metabolite channeling. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10547-10552. PMID: 9724740, PMCID: PMC27931, DOI: 10.1073/pnas.95.18.10547.Peer-Reviewed Original ResearchConceptsPhosphotransferase systemMacromolecular crowdingFlux response coefficientsCarbohydrate phosphotransferase systemGlucose phosphotransferase systemBacterial phosphoenolpyruvateSignal transductionMetabolite channelingCell-free extractsProtein concentrationEnzyme complexCarbohydrate phosphorylationPEG 6000 concentrationPolyethylene glycol 6000Total enzyme concentrationHigh protein concentrationsEscherichia coliLow protein concentrationsForm complexesGroup-transfer pathwaysMetabolic behaviorPEG 6000Crowded environmentsSubtleties in control by metabolic channelling and enzyme organization
Kholodenko B, Rohwer J, Cascante M, Westerhoff H. Subtleties in control by metabolic channelling and enzyme organization. Molecular And Cellular Biochemistry 1998, 184: 311-320. PMID: 9746327, DOI: 10.1023/a:1006809028612.Peer-Reviewed Original ResearchConceptsPhosphotransferase systemMetabolic control analysisBacterial phosphotransferase systemEnzyme-enzyme associationsDirect metabolite transferEnzyme organizationConcomitant phosphorylationTernary complex formationPTS pathwayMetabolite transferEnzyme sequestrationMetabolic channellingLiving cellsMacromolecular crowdingPhosphoryl groupEnzyme controlTernary complexCell functionPathwayEnzymeComplex formationIdeal systemEnzyme control coefficientsMetabolic systemsControl coefficientsSubtleties in control by metabolic channelling and enzyme organization
Kholodenko B, Rohwer J, Cascante M, Westerhoff H. Subtleties in control by metabolic channelling and enzyme organization. Developments In Molecular And Cellular Biochemistry 1998, 311-320. DOI: 10.1007/978-1-4615-5653-4_20.Peer-Reviewed Original ResearchPhosphotransferase systemMetabolic control analysisBacterial phosphotransferase systemEnzyme-enzyme associationsDirect metabolite transferEnzyme organizationConcomitant phosphorylationTernary complex formationPTS pathwayMetabolite transferEnzyme sequestrationMetabolic channellingLiving cellsMacromolecular crowdingPhosphoryl groupEnzyme controlTernary complexCell functionPathwayEnzymeComplex formationIdeal systemEnzyme control coefficientsMetabolic systemsControl coefficients