1998
Implications of macromolecular crowding for signal transduction and metabolite channeling
Rohwer J, Postma P, Kholodenko B, Westerhoff H. Implications of macromolecular crowding for signal transduction and metabolite channeling. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 10547-10552. PMID: 9724740, PMCID: PMC27931, DOI: 10.1073/pnas.95.18.10547.Peer-Reviewed Original ResearchConceptsPhosphotransferase systemMacromolecular crowdingFlux response coefficientsCarbohydrate phosphotransferase systemGlucose phosphotransferase systemBacterial phosphoenolpyruvateSignal transductionMetabolite channelingCell-free extractsProtein concentrationEnzyme complexCarbohydrate phosphorylationPEG 6000 concentrationPolyethylene glycol 6000Total enzyme concentrationHigh protein concentrationsEscherichia coliLow protein concentrationsForm complexesGroup-transfer pathwaysMetabolic behaviorPEG 6000Crowded environments
1996
Molecular Control Analysis: Control within Proteins and Molecular Processes
Brown G, Westerhoff H, Kholodenko B. Molecular Control Analysis: Control within Proteins and Molecular Processes. Journal Of Theoretical Biology 1996, 182: 389-396. PMID: 8944172, DOI: 10.1006/jtbi.1996.0178.Peer-Reviewed Original Research
1995
How to reveal various aspects of regulation in group-transfer pathways
Kholodenko B, Westerhoff H. How to reveal various aspects of regulation in group-transfer pathways. Biochimica Et Biophysica Acta (BBA) - Bioenergetics 1995, 1229: 275-289. DOI: 10.1016/0005-2728(95)00013-9.Peer-Reviewed Original ResearchGroup-transfer pathwaysEnzyme-enzyme complexPathway componentsGene expressionMetabolic pathwaysAspects of regulationGenetic modulationSpecific inhibitorSuch inhibitorsEnzyme concentrationNon-competitive inhibitorEnzymeControl coefficientsPathwayIntact systemInhibitorsInhibited enzymeRegulationExpression
1993
The sum of the control coefficients of all enzymes on the flux through a group‐transfer pathway can be as high as two
van DAM K, van der VLAG J, KHOLODENKO B, WESTERHOFF H. The sum of the control coefficients of all enzymes on the flux through a group‐transfer pathway can be as high as two. The FEBS Journal 1993, 212: 791-799. PMID: 8462550, DOI: 10.1111/j.1432-1033.1993.tb17720.x.Peer-Reviewed Original ResearchConceptsGroup-transfer pathwaysReaction activityDissociation rate constantsElectron transfer chainAcceptor couplesEnzyme-enzyme interactionsEnzyme concentrationRate constantsEnzyme-enzyme complexEnzyme interactionsComplexesControl of enzymesEnzyme complexEnzymeConcentrationBacterial phosphoenolpyruvateMain pathwayInteractionConstantsChainControl analysisSimple metabolic pathwaysSugar phosphotransferase system