Featured Publications
The dynamic disulphide relay of quiescin sulphydryl oxidase
Alon A, Grossman I, Gat Y, Kodali V, DiMaio F, Mehlman T, Haran G, Baker D, Thorpe C, Fass D. The dynamic disulphide relay of quiescin sulphydryl oxidase. Nature 2012, 488: 414-418. PMID: 22801504, PMCID: PMC3521037, DOI: 10.1038/nature11267.Peer-Reviewed Original ResearchIdentification of the gene that codes for the σ(2) receptor.
Alon A, Schmidt HR, Wood MD, Sahn JJ, Martin SF, Kruse AC. Identification of the gene that codes for the σ(2) receptor. Proc Natl Acad Sci U S A 2017, 114: 7160-7165. PMID: 28559337, DOI: 10.1073/pnas.1705154114.Peer-Reviewed Original ResearchStructures of the σ(2) receptor enable docking for bioactive ligand discovery.
Alon A, Lyu J, Braz JM, Tummino TA, Craik V, O'Meara MJ, Webb CM, Radchenko DS, Moroz YS, Huang XP, Liu Y, Roth BL, Irwin JJ, Basbaum AI, Shoichet BK, Kruse AC. Structures of the σ(2) receptor enable docking for bioactive ligand discovery. Nature 2021, 600: 759-764. PMID: 34880501, DOI: 10.1038/s41586-021-04175-x.Peer-Reviewed Original Research
2022
The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination.
Gao Y, Barajas-Ornelas RDC, Amon JD, Ramírez-Guadiana FH, Alon A, Brock KP, Marks DS, Kruse AC, Rudner DZ. The SpoVA membrane complex is required for dipicolinic acid import during sporulation and export during germination. Genes Dev 2022, 36: 634-646. PMID: 35654455, DOI: 10.1101/gad.349488.122.Peer-Reviewed Original Research
2021
The Spliced Leader RNA Silencing (SLS) Pathway in Trypanosoma brucei Is Induced by Perturbations of Endoplasmic Reticulum, Golgi Complex, or Mitochondrial Protein Factors: Functional Analysis of SLS-Inducing Kinase PK3.
Okalang U, Mualem Bar-Ner B, Rajan KS, Friedman N, Aryal S, Egarmina K, Hope R, Khazanov N, Senderowitz H, Alon A, Fass D, Michaeli S. The Spliced Leader RNA Silencing (SLS) Pathway in Trypanosoma brucei Is Induced by Perturbations of Endoplasmic Reticulum, Golgi Complex, or Mitochondrial Protein Factors: Functional Analysis of SLS-Inducing Kinase PK3. MBio 2021, 12: e0260221. PMID: 34844425, DOI: 10.1128/mBio.02602-21.Peer-Reviewed Original ResearchDormant spores sense amino acids through the B subunits of their germination receptors.
Artzi L, Alon A, Brock KP, Green AG, Tam A, Ramírez-Guadiana FH, Marks D, Kruse A, Rudner DZ. Dormant spores sense amino acids through the B subunits of their germination receptors. Nat Commun 2021, 12: 6842. PMID: 34824238, DOI: 10.1038/s41467-021-27235-2.Peer-Reviewed Original ResearchDrug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2.
Tummino TA, Rezelj VV, Fischer B, Fischer A, O'Meara MJ, Monel B, Vallet T, White KM, Zhang Z, Alon A, Schadt H, O'Donnell HR, Lyu J, Rosales R, McGovern BL, Rathnasinghe R, Jangra S, Schotsaert M, Galarneau JR, Krogan NJ, Urban L, Shokat KM, Kruse AC, García-Sastre A, Schwartz O, Moretti F, Vignuzzi M, Pognan F, Shoichet BK. Drug-induced phospholipidosis confounds drug repurposing for SARS-CoV-2. Science 2021, 373: 541-547. PMID: 34326236, DOI: 10.1126/science.abi4708.Peer-Reviewed Original ResearchCOVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms.
Malone RW, Tisdall P, Fremont-Smith P, Liu Y, Huang XP, White KM, Miorin L, Moreno E, Alon A, Delaforge E, Hennecker CD, Wang G, Pottel J, Blair RV, Roy CJ, Smith N, Hall JM, Tomera KM, Shapiro G, Mittermaier A, Kruse AC, García-Sastre A, Roth BL, Glasspool-Malone J, Ricke DO. COVID-19: Famotidine, Histamine, Mast Cells, and Mechanisms. Front Pharmacol 2021, 12: 633680. PMID: 33833683, DOI: 10.3389/fphar.2021.633680.Peer-Reviewed Original Research
2019
cis-Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures.
Javitt G, Grossman-Haham I, Alon A, Resnick E, Mutsafi Y, Ilani T, Fass D. cis-Proline mutants of quiescin sulfhydryl oxidase 1 with altered redox properties undermine extracellular matrix integrity and cell adhesion in fibroblast cultures. Protein Sci 2019, 28: 228-238. PMID: 30367560, DOI: 10.1002/pro.3537.Peer-Reviewed Original Research
2017
Structural Perspectives on Sigma-1 Receptor Function.
Alon A, Schmidt H, Zheng S, Kruse AC. Structural Perspectives on Sigma-1 Receptor Function. Adv Exp Med Biol 2017, 964: 5-13. PMID: 28315261, DOI: 10.1007/978-3-319-50174-1_2.Peer-Reviewed Original Research
2015
AbDesign: An algorithm for combinatorial backbone design guided by natural conformations and sequences.
Lapidoth GD, Baran D, Pszolla GM, Norn C, Alon A, Tyka MD, Fleishman SJ. AbDesign: An algorithm for combinatorial backbone design guided by natural conformations and sequences. Proteins 2015, 83: 1385-406. PMID: 25670500, DOI: 10.1002/prot.24779.Peer-Reviewed Original Research
2013
An inhibitory antibody blocks the first step in the dithiol/disulfide relay mechanism of the enzyme QSOX1.
Grossman I, Alon A, Ilani T, Fass D. An inhibitory antibody blocks the first step in the dithiol/disulfide relay mechanism of the enzyme QSOX1. J Mol Biol 2013, 425: 4366-78. PMID: 23867277, DOI: 10.1016/j.jmb.2013.07.011.Peer-Reviewed Original ResearchA secreted disulfide catalyst controls extracellular matrix composition and function.
Ilani T, Alon A, Grossman I, Horowitz B, Kartvelishvily E, Cohen SR, Fass D. A secreted disulfide catalyst controls extracellular matrix composition and function. Science 2013, 341: 74-6. PMID: 23704371, DOI: 10.1126/science.1238279.Peer-Reviewed Original Research
2012
Phylogenetics and enzymology of plant quiescin sulfhydryl oxidase.
Limor-Waisberg K, Alon A, Mehlman T, Fass D. Phylogenetics and enzymology of plant quiescin sulfhydryl oxidase. FEBS Lett 2012, 586: 4119-25. PMID: 23068612, DOI: 10.1016/j.febslet.2012.10.003.Peer-Reviewed Original ResearchExploring ORFan domains in giant viruses: structure of mimivirus sulfhydryl oxidase R596.
Hakim M, Ezerina D, Alon A, Vonshak O, Fass D. Exploring ORFan domains in giant viruses: structure of mimivirus sulfhydryl oxidase R596. PLoS One 2012, 7: e50649. PMID: 23209798, DOI: 10.1371/journal.pone.0050649.Peer-Reviewed Original Research
2011
Improved molecular replacement by density- and energy-guided protein structure optimization
DiMaio F, Terwilliger T, Read R, Wlodawer A, Oberdorfer G, Wagner U, Valkov E, Alon A, Fass D, Axelrod H, Das D, Vorobiev S, Iwaï H, Pokkuluri P, Baker D. Improved molecular replacement by density- and energy-guided protein structure optimization. Nature 2011, 473: 540-543. PMID: 21532589, PMCID: PMC3365536, DOI: 10.1038/nature09964.Peer-Reviewed Original Research
2010
QSOX contains a pseudo-dimer of functional and degenerate sulfhydryl oxidase domains.
Alon A, Heckler EJ, Thorpe C, Fass D. QSOX contains a pseudo-dimer of functional and degenerate sulfhydryl oxidase domains. FEBS Lett 2010, 584: 1521-5. PMID: 20211621, DOI: 10.1016/j.febslet.2010.03.001.Peer-Reviewed Original Research
2008
Human quiescin-sulfhydryl oxidase, QSOX1: probing internal redox steps by mutagenesis.
Heckler EJ, Alon A, Fass D, Thorpe C. Human quiescin-sulfhydryl oxidase, QSOX1: probing internal redox steps by mutagenesis. Biochemistry 2008, 47: 4955-63. PMID: 18393449, DOI: 10.1021/bi702522q.Peer-Reviewed Original Research