2014
The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain
Rawling DC, Kohlway AS, Luo D, Ding SC, Pyle AM. The RIG-I ATPase core has evolved a functional requirement for allosteric stabilization by the Pincer domain. Nucleic Acids Research 2014, 42: 11601-11611. PMID: 25217590, PMCID: PMC4191399, DOI: 10.1093/nar/gku817.Peer-Reviewed Original ResearchConceptsATPase coreRetinoic acid-inducible gene IAcid-inducible gene INon-self RNASeries of mutationsActivity of RIGMetazoan cellsHelicase coreAllosteric controlTerminal domainPattern recognition receptorsAlpha-helixBiophysical analysisGene IAllosteric stabilizationType I interferonEnzymatic activityRecognition receptorsViral RNAStructural studiesRNAI interferonAdjacent domainsDomainImportant roleThe Linker Region of NS3 Plays a Critical Role in the Replication and Infectivity of Hepatitis C Virus
Kohlway A, Pirakitikulr N, Ding SC, Yang F, Luo D, Lindenbach BD, Pyle AM. The Linker Region of NS3 Plays a Critical Role in the Replication and Infectivity of Hepatitis C Virus. Journal Of Virology 2014, 88: 10970-10974. PMID: 24965468, PMCID: PMC4178846, DOI: 10.1128/jvi.00745-14.Peer-Reviewed Original Research
2013
Defining the functional determinants for RNA surveillance by RIG‐I
Kohlway A, Luo D, Rawling DC, Ding SC, Pyle AM. Defining the functional determinants for RNA surveillance by RIG‐I. EMBO Reports 2013, 14: 772-779. PMID: 23897087, PMCID: PMC3790051, DOI: 10.1038/embor.2013.108.Peer-Reviewed Original ResearchConceptsMelanoma differentiation-associated gene 5Robust ATPase activityDuplex RNA substrateMinimal functional unitATPase activityRetinoic acid-inducible geneInnate immune machineryAcid-inducible geneRNA surveillanceDifferentiation-associated gene 5RNA substratesIntracellular RNA sensorsDuplex RNARNA complexRNA targetsGene 5RNA virusesDistinct conformationsRNA sensorsDsRNA complexImmune machineryRNA duplexesInterferon responseFunctional determinantsFunctional units