Pooled Exome Analysis
Sequencing costs for specific applications:
Recent R&D efforts at YCGA for analyzing pooled samples has resulted in increased throughput and significant cost savings for various applications. Since one lane of a NovaSeq flowcell provides sequence for approximately 500 billion bases, it is possible to sequence multiple barcoded samples in one lane and still obtain sufficient coverage for each of the pooled samples. The following table provides costs for the most common applications. We guarantee 95% 10x target base coverage for germline, 95% 20x target base coverage for de novo mutations, and 95% 40x target base coverage for somatic mutations in tumors. If you need further information, please contact us.
Whole-Exome Analysis:
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# of barcoded exomes captured
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Example of
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Approx. # of millions of
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Cost*/exome |
5T 10N |
Detection of somatic variations/
|
150T 75N |
$602T $362N |
8 |
Identification of denovo mutations |
80-100 |
$420 |
16 |
Discovery of Mendelian/
|
30-40 |
$270 |
*Yale Prices for FY2018