Pooled Exome Analysis

Sequencing costs for specific applications:
Recent R&D efforts at YCGA for analyzing pooled samples has resulted in increased throughput and significant cost savings for various applications. Since one lane of a NovaSeq flowcell provides sequence for approximately 500 billion bases, it is possible to sequence multiple barcoded samples in one lane and still obtain sufficient coverage for each of the pooled samples. The following table provides costs for the most common applications. We guarantee 95% 10x target base coverage for germline, 95% 20x target base coverage for de novo mutations, and 95% 40x target base coverage for  somatic mutations in  tumors. If you need further information, please contact us.

Whole-Exome Analysis:          
As indicated in the table below, the cost depends upon how many samples are pooled together.

# of barcoded exomes captured
per one oligo pool          

Example of
Applications/Projects

Approx. # of millions of
PE reads/exome

Cost*/exome

5T 10N

Detection of somatic variations/
Tumor/Normal samples

150T 75N

$602T  $362N

8

Identification of denovo mutations

80-100

$420

16

Discovery of Mendelian/
rare disease variations
and common disorders

30-40

$270

*Yale Prices for FY2018