Custom Synthesis and Clone Construction
What is BioXp™ Custom Cloning?
DNA fragments built on the BioXp™ system are directly cloned into a customer supplied vector using Gibson Assembly® methodology. This application eliminates subcloning, saving time and effort.
How do I prepare for my BioXpTM Custom Cloning submission?
DNA fragments and vectors need to be designed with homologous overlaps to enable Gibson Assembly® cloning on the BioXp™ system. Vector linearization prep protocols can be found here.
How do I submit my sequences?
Instructions for sequence submission can be found here.
How will I receive my clones?
Clones will be delivered in a 96-tube PCR plate along with a plate map showing their locations.
Who can I contact regarding questions?
Joseph DeLuca - Oligo Resource Director