2020
Molecular Characterization and Experimental Utility of Monoclonal Antibodies with Specificity for Aliphatic Di- and Polyisocyanates
Wisnewski AV, Liu J. Molecular Characterization and Experimental Utility of Monoclonal Antibodies with Specificity for Aliphatic Di- and Polyisocyanates. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 2020, 39: 66-73. PMID: 32302507, PMCID: PMC7310211, DOI: 10.1089/mab.2020.0006.Peer-Reviewed Original ResearchConceptsBALB/c miceMonoclonal antibodiesOccupational asthmaC miceSerologic detectionDifferent carrier proteinsNew mAbsEpitope specificityUseful research reagentsDisease preventionImmune recognitionStable hybridomasWestern blotAlbumin adductsMouse skinMAbsSurveillance approachSurveillance effortsAntibodiesMolecular determinantsHybridomasAliphatic diComplementary determining regionsMolecular characterizationResearch reagents
2013
Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate
Wisnewski AV, Liu J. Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Molecular Immunology 2013, 54: 233-237. PMID: 23295252, PMCID: PMC3563841, DOI: 10.1016/j.molimm.2012.11.017.Peer-Reviewed Original ResearchConceptsEpitope specificityAutologous serum proteinsLow molecular weight chemicalsOccupational asthmaOccupational allergensMolecular determinantsDisease pathogenesisSecreting clonesCDR3 regionWestern blotGene usageMonoclonal antibodiesMajor causeWeight chemicalsIgG1 mAbGermline gene usageHuman albuminImmune specificityMAbsSerum proteinsELISADiagnostic reagentsUseful diagnostic reagentHybridomasSomatic hypermutation
1992
Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens
Klock G, Wisnewski AV, el-Bassiouni EA, Ramadan MI, Gessner P, Zimmermann U, Kresina TF. Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1992, 11: 469-481. PMID: 1398684, DOI: 10.1089/hyb.1992.11.469.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesIgG antibodiesParasite antigensHypo-osmolar electrofusionS. mansoniSchistosoma mansoni wormsCell linesWestern blot analysisEgg antigenParasitic antigensAntigen reactivityAntigenAntibodiesSplenocytesBlot analysisHybridomasCandidate moleculesMansoniHybridoma generationMitogenCell fusionSomatic cell fusionGreater numberELISA