2002
Effects of hexamethylene diisocyanate exposure on human airway epithelial cells: in vitro cellular and molecular studies.
Wisnewski AV, Liu Q, Miller JJ, Magoski N, Redlich CA. Effects of hexamethylene diisocyanate exposure on human airway epithelial cells: in vitro cellular and molecular studies. Environmental Health Perspectives 2002, 110: 901-907. PMID: 12204825, PMCID: PMC1240990, DOI: 10.1289/ehp.02110901.Peer-Reviewed Original ResearchConceptsHuman airway epithelial cellsImmunofluorescence analysisAirway epithelial cellsEpithelial cellsThiol redox homeostasisThioredoxin reductase expressionCellular stressEpithelial cell lineSensitive genesMicroarray analysisNorthern analysisHexamethylene diisocyanate exposureGenesAirway epithelial cell lineMolecular levelMolecular studiesDihydrodiol dehydrogenaseReductase expressionHDI exposureSubcytotoxic concentrationsCell linesMessage levelsImportant sensorMRNA levelsCells
1999
Isocyanate-conjugated human lung epithelial cell proteins: A link between exposure and asthma?
Wisnewski A, Lemus R, Karol M, Redlich C. Isocyanate-conjugated human lung epithelial cell proteins: A link between exposure and asthma? Journal Of Allergy And Clinical Immunology 1999, 104: 341-347. PMID: 10452755, DOI: 10.1016/s0091-6749(99)70377-5.Peer-Reviewed Original ResearchConceptsIsocyanate-induced asthmaHuman lung epithelial cell lineEpithelial cell proteinsLung epithelial cell lineEpithelial cell lineLymphocyte proliferationProliferation of PBMCCell linesAirway inflammationAtopic subjectsNonasthmatic individualsHDI exposureAirway epitheliumImmunostimulatory potentialAsthmaCell proteinsWestern blottingPotential roleProliferationExposureHDI concentrationCross-linking chemicalsCell polypeptidesConjugation patternSubjects
1997
In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies
Elliot BC, Wisnewski AV, Johnson J, Fenwick-Smith D, Wiest P, Hamer D, Kresina T, Flanigan TP. In vitro inhibition of Cryptosporidium parvum infection by human monoclonal antibodies. Infection And Immunity 1997, 65: 3933-3935. PMID: 9284173, PMCID: PMC175560, DOI: 10.1128/iai.65.9.3933-3935.1997.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyC. parvum infectionParvum infectionEnzyme-linked immunosorbent assayMonoclonal antibodiesHuman immunodeficiency virus-seronegative womenCell linesHuman enterocyte cell lineHigher specific antibody titersCryptosporidium parvum infectionAnti-Cryptosporidium antibodiesPeripheral blood lymphocytesSpecific antibody titersMonoclonal cell linesEnterocyte cell lineAntibody-secreting clonesC. parvum sporozoitesImmunoglobulin therapyAntibody titersBlood lymphocytesFatal illnessPotential therapyHypo-osmolar electrofusionTumor cell linesInfection
1992
Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens
Klock G, Wisnewski AV, el-Bassiouni EA, Ramadan MI, Gessner P, Zimmermann U, Kresina TF. Human Hybridoma Generation by Hypo-Osmolar Electrofusion: Characterization of Human Monoclonal Antibodies to Schistosoma mansoni Parasite Antigens. Monoclonal Antibodies In Immunodiagnosis And Immunotherapy 1992, 11: 469-481. PMID: 1398684, DOI: 10.1089/hyb.1992.11.469.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyMonoclonal antibodiesIgG antibodiesParasite antigensHypo-osmolar electrofusionS. mansoniSchistosoma mansoni wormsCell linesWestern blot analysisEgg antigenParasitic antigensAntigen reactivityAntigenAntibodiesSplenocytesBlot analysisHybridomasCandidate moleculesMansoniHybridoma generationMitogenCell fusionSomatic cell fusionGreater numberELISA